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First Isolation and Rapid Identification of Newcastle Disease Virus from Aborted Fetus of Dromedary Camel Using Next-Generation Sequencing

1
Department of Microbiology, The University of Hong Kong, Hong Kong, China
2
State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China
3
Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China
4
Central Veterinary Research Laboratory, Dubai 00000, UAE
5
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the manuscript.
Viruses 2019, 11(9), 810; https://doi.org/10.3390/v11090810
Received: 1 July 2019 / Revised: 23 August 2019 / Accepted: 30 August 2019 / Published: 1 September 2019
(This article belongs to the Section Animal Viruses)
Newcastle disease virus (NDV) causes morbidities and mortalities in wild and domestic birds globally. For humans, exposure to infected birds can cause conjunctivitis and influenza-like symptoms. NDV infections in mammals are rarely reported. In this study, using next-generation sequencing, an NDV was identified and isolated from Vero cells inoculated with the nasal swab of an aborted dromedary fetus in Dubai, during the time when an NDV outbreak occurred in a pigeon farm located in close proximity to the dairy camel farm where the mother of the aborted dromedary fetus resided, and there were a lot of pigeons in the camel farm. Genome analysis revealed that the structurally and functionally important features of other NDVs were also present in this dromedary NDV genome. Phylogenetic analysis based on the nucleotide sequences of fusion protein (F), hemagglutinin-neuraminidase protein (HN) and complete polyprotein showed that the virus belonged to sub-genotype VIg of class II NDV and is most closely related to pigeon NDVs in Egypt in the same year. The present study is the first that demonstrated isolation of NDV in dromedaries. Further study is warranted to investigate the relationship between NDV infection and abortion. View Full-Text
Keywords: Newcastle Disease virus; aborted fetus; dromedary Newcastle Disease virus; aborted fetus; dromedary
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MDPI and ACS Style

Teng, J.L.L.; Wernery, U.; Lee, H.H.; Joseph, S.; Fung, J.; Elizabeth, S.K.; Yeong, K.Y.; Kinne, J.; Chan, K.-H.; Lau, S.K.P.; Woo, P.C.Y. First Isolation and Rapid Identification of Newcastle Disease Virus from Aborted Fetus of Dromedary Camel Using Next-Generation Sequencing. Viruses 2019, 11, 810. https://doi.org/10.3390/v11090810

AMA Style

Teng JLL, Wernery U, Lee HH, Joseph S, Fung J, Elizabeth SK, Yeong KY, Kinne J, Chan K-H, Lau SKP, Woo PCY. First Isolation and Rapid Identification of Newcastle Disease Virus from Aborted Fetus of Dromedary Camel Using Next-Generation Sequencing. Viruses. 2019; 11(9):810. https://doi.org/10.3390/v11090810

Chicago/Turabian Style

Teng, Jade Lee Lee, Ulrich Wernery, Hwei Huih Lee, Sunitha Joseph, Joshua Fung, Shyna Korah Elizabeth, Kai Yan Yeong, Joerg Kinne, Kwok-Hung Chan, Susanna Kar Pui Lau, and Patrick Chiu Yat Woo. 2019. "First Isolation and Rapid Identification of Newcastle Disease Virus from Aborted Fetus of Dromedary Camel Using Next-Generation Sequencing" Viruses 11, no. 9: 810. https://doi.org/10.3390/v11090810

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