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Open AccessArticle

Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

1
Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, Israel
2
The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 761001, Israel
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(9), 802; https://doi.org/10.3390/v11090802
Received: 5 August 2019 / Revised: 27 August 2019 / Accepted: 29 August 2019 / Published: 29 August 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV. View Full-Text
Keywords: Closteroviridae; Crinivirus; Bemisia tabaci; chlorosis; shoot propagation Closteroviridae; Crinivirus; Bemisia tabaci; chlorosis; shoot propagation
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Hadad, L.; Luria, N.; Smith, E.; Sela, N.; Lachman, O.; Dombrovsky, A. Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production. Viruses 2019, 11, 802.

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  • Supplementary File 1:

    ZIP-Document (ZIP, 540 KB)

  • Externally hosted supplementary file 1
    Doi: 10.5281/zenodo.3360655
    Description: Table S1. Primer pairs used for sequencing cannabis lettuce chlorosis virus (LCV) isolate.
  • Externally hosted supplementary file 2
    Doi: 10.5281/zenodo.3360655
    Description: Table S2. Overlapping primer pairs used for sequencing cannabis lettuce chlorosis virus (LCV) isolate.
  • Externally hosted supplementary file 3
    Doi: 10.5281/zenodo.3360655
    Description: Figure S1 Analysis of cannabis lettuce chlorosis virus (LCV-Can) seed transmission. (a) LCV-Can in seeds of symptomatic cannabis plants. Lanes 1-5, RT-PCR with primer set No. 8, (Table S1). Lanes 6-10, RT-PCR with primer set No. 13 (Table S1). Lanes 4,9- symptomatic cannabis plants. Lanes 1-3, 6-8-seeds of symptomatic cannabis plants,100 seeds in each lane. Lanes 5,10- no template control (NTC). (b) LCV-Can seed to seedling transmission. Lanes 1-4, RT-PCR with primer set No. 8, (Table S1). Lanes 5-8, RT-PCR with primer set No. 13 (Table S1). Lanes 1,5- symptomatic cannabis plants. Lanes 2,6- pooled 10 seeds of the symptomatic plant. Lanes 3,7- pooled 8 seedlings of the sown seeds from the symptomatic plant. Lanes 4,8- no template control (NTC).
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