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Open AccessArticle

Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade

1
Sichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China
2
National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China
3
Department of Plant Protection, Sindh Agriculture University, Tandojam 70060, Pakistan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(9), 793; https://doi.org/10.3390/v11090793
Received: 4 July 2019 / Revised: 20 August 2019 / Accepted: 26 August 2019 / Published: 29 August 2019
(This article belongs to the Special Issue The Complexity of the Potyviral Interaction Network)
Shading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus (SMV) is a biotic factor that limits the yield and quality of soybean. However, little is known about the defense response of soybean to SMV in the shade. Thus, in the current study, both intensity and quality (red:far-red, R:FR) of the light were changed to simulate the shaded environment and comparative transcriptome analysis was performed. Morphologically, plant growth was inhibited by SMV, which decreased 35.93% of plant height and 8.97% of stem diameter in the shade. A total of 3548 and 4319 differentially expressed genes (DEGs) were identified in soybean plants infected with SMV under normal light and in the shade. Enrichment analysis showed that the plant defense-related genes were upregulated under normal light but downregulated in the shade. Pathways that were repressed include plant-pathogen interaction, secondary metabolism, sugar metabolism, and vitamin metabolism. In addition, genes associated with signaling pathways such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH) were also downregulated in the shade. A qRT-PCR assay of 15 DEGs was performed to confirm transcriptome results. According to our knowledge, this is the first report on soybean response to dual stress factors. These results provide insights into the molecular mechanisms in which soybean plants were infected with SMV in the shade. View Full-Text
Keywords: soybean; soybean mosaic virus; shading; RNA-Seq; plant-pathogen interaction soybean; soybean mosaic virus; shading; RNA-Seq; plant-pathogen interaction
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MDPI and ACS Style

Zhang, L.; Shang, J.; Wang, W.; Du, J.; Li, K.; Wu, X.; Yu, L.; Liu, C.; Khaskheli, M.I.; Yang, W. Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade. Viruses 2019, 11, 793. https://doi.org/10.3390/v11090793

AMA Style

Zhang L, Shang J, Wang W, Du J, Li K, Wu X, Yu L, Liu C, Khaskheli MI, Yang W. Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade. Viruses. 2019; 11(9):793. https://doi.org/10.3390/v11090793

Chicago/Turabian Style

Zhang, Lei; Shang, Jing; Wang, Wenming; Du, Junbo; Li, Kai; Wu, Xiaoling; Yu, Liang; Liu, Chunyan; Khaskheli, Muhammad I.; Yang, Wenyu. 2019. "Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade" Viruses 11, no. 9: 793. https://doi.org/10.3390/v11090793

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