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Open AccessTechnical Note

Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

1
Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
2
Ecole Doctorale Science de la Vie et de l’Environnement, Faculté des Sciences, Université d’Antananarivo, 101 Antananarivo, Madagascar
3
Plague Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
4
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 3FX, UK
5
Département de Biochimie Fondamentale et Appliquée, Faculté des Sciences, Université d’Antananarivo, 101 Antananarivo, Madagascar
6
Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, 69000 Lyon, France
*
Author to whom correspondence should be addressed.
Present address: Centre National de Référence des Hantavirus, Unité Environnement et Risques Infectieux, Institut Pasteur, 75015 Paris, France.
Viruses 2019, 11(8), 718; https://doi.org/10.3390/v11080718
Received: 28 May 2019 / Revised: 15 July 2019 / Accepted: 19 July 2019 / Published: 6 August 2019
(This article belongs to the Special Issue Hantaviruses)
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PDF [247 KB, uploaded 22 August 2019]
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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%. View Full-Text
Keywords: Hantavirus; Thailand orthohantavirus; Real-time RT-PCR Hantavirus; Thailand orthohantavirus; Real-time RT-PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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MDPI and ACS Style

Raharinosy, V.; Heraud, J.-M.; Rabemananjara, H.A.; Telfer, S.; Rakoto, D.A.D.; Filippone, C.; Reynes, J.-M. Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay. Viruses 2019, 11, 718.

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