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Open AccessTechnical Note

Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

1
Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
2
Ecole Doctorale Science de la Vie et de l’Environnement, Faculté des Sciences, Université d’Antananarivo, 101 Antananarivo, Madagascar
3
Plague Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
4
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 3FX, UK
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Département de Biochimie Fondamentale et Appliquée, Faculté des Sciences, Université d’Antananarivo, 101 Antananarivo, Madagascar
6
Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, 69000 Lyon, France
*
Author to whom correspondence should be addressed.
Present address: Centre National de Référence des Hantavirus, Unité Environnement et Risques Infectieux, Institut Pasteur, 75015 Paris, France.
Viruses 2019, 11(8), 718; https://doi.org/10.3390/v11080718
Received: 28 May 2019 / Revised: 15 July 2019 / Accepted: 19 July 2019 / Published: 6 August 2019
(This article belongs to the Special Issue Hantaviruses)
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%. View Full-Text
Keywords: Hantavirus; Thailand orthohantavirus; Real-time RT-PCR Hantavirus; Thailand orthohantavirus; Real-time RT-PCR
MDPI and ACS Style

Raharinosy, V.; Heraud, J.-M.; Rabemananjara, H.A.; Telfer, S.; Rakoto, D.A.D.; Filippone, C.; Reynes, J.-M. Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay. Viruses 2019, 11, 718.

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