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Open AccessArticle

Application of the Phage Lysin Ply5218 in the Treatment of Streptococcus suis Infection in Piglets

1
Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
2
Shanghai Animal Disease Control Centre, Shanghai 201103, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(8), 715; https://doi.org/10.3390/v11080715
Received: 24 June 2019 / Revised: 1 August 2019 / Accepted: 2 August 2019 / Published: 5 August 2019
Streptococcus suis (S. suis) is a gram-positive bacterium and zoonotic pathogen. Currently it poses a serious problem in the swine industry due to the emergence of antibiotic-resistant bacteria. Thus, novel antimicrobials against S. suis infections are urgently needed. In the previous study, a cell wall hydrolase or lysin derived from Streptococcus prophage phi5218, termed Ply5218, was identified. This lysin showed strong bacteriolytic activity against S. suis. In the current study, the in vitro data showed that after incubation with pig serum, the bacteriolytic efficacy of Ply5218 declined in a time-dependent manner. The in vivo assays indicated that a Ply5218 triple treatment (6, 24, and 48 h post infection) was effective against various serotypes of S. suis in a murine infection model. This regimen also alleviated streptococcal-induced clinical symptoms in piglets and significantly reduced the bacterial burden and levels of interleukin 6, a proinflammatory cytokine. This study indicates that Ply5218 shows strong antibacterial activity in pigs and has the potential to be used as a treatment for infectious diseases caused by S. suis. View Full-Text
Keywords: Streptococcus suis; lysin; piglet; infection; treatment Streptococcus suis; lysin; piglet; infection; treatment
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Wang, Z.; Ma, J.; Wang, J.; Yang, D.; Kong, L.; Fu, Q.; Cheng, Y.; Wang, H.; Yan, Y.; Sun, J. Application of the Phage Lysin Ply5218 in the Treatment of Streptococcus suis Infection in Piglets. Viruses 2019, 11, 715.

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