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Open AccessArticle

Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins

1
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, China
2
College of Animal Medicine, Yunnan Agricultural University, Black Dragon Pool, Kunming 650201, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(6), 544; https://doi.org/10.3390/v11060544
Received: 16 May 2019 / Revised: 3 June 2019 / Accepted: 10 June 2019 / Published: 11 June 2019
(This article belongs to the Section Animal Viruses)
Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with PCV2 infection in vitro and in vivo. HMGCR inhibits the early stages of PCV2 infection, while PCV2 infection induces the phosphorylation of HMGCR to inactivate the protein. In this study, we investigated the possibility that adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK), and protein phosphatase 2 (PP2A) participate in HMGCR-mediated inhibition of PCV2 infection and the interaction of porcine HMGCR with PCV2 proteins. The results showed that AMPK activity fluctuated in cells during the early stage of PCV2 infection, while PP2A had little effect on PCV2 infection and HMGCR activity. Furthermore, PCV2 infection may enhance or maintain the level of phosphorylated HMGCR by directly interacting with the protein in PK-15 cells. These findings may provide a better understanding of PCV2 pathogenesis, and HMGCR may be a novel PCV2 antiviral target. View Full-Text
Keywords: porcine circovirus type 2 (PCV2); 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR); adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK); protein phosphatase 2 (PP2A); interaction porcine circovirus type 2 (PCV2); 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR); adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK); protein phosphatase 2 (PP2A); interaction
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Ouyang, T.; Niu, G.; Zhang, Y.; Liu, X.; Zhang, X.; Zhang, S.; Geng, Y.; Pang, D.; Ouyang, H.; Ren, L. Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins. Viruses 2019, 11, 544.

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