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Open AccessArticle

Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness

1
National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan
2
Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan
3
College of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
4
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan
5
Animal Health Research Institutes, Danshui, New Taipei City 25158, Taiwan
6
Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan
7
Institute of Preventive Medicine, National Defence Medical Centre, Taipei 23742, Taiwan
*
Author to whom correspondence should be addressed.
Equal contribution
Viruses 2019, 11(6), 543; https://doi.org/10.3390/v11060543
Received: 23 April 2019 / Revised: 4 June 2019 / Accepted: 5 June 2019 / Published: 11 June 2019
(This article belongs to the Section Antivirals & Vaccines)
Novel low-pathogenic avian influenza (LPAI) H5N2 viruses hit poultry farms in Taiwan in 2003, and evolved into highly pathogenic avian influenza (HPAI) viruses in 2010. These viruses are reassortant viruses containing HA and NA genes from American-lineage H5N2 and six internal genes from local H6N1 viruses. According to a serological survey, the Taiwan H5N2 viruses can cause asymptomatic infections in poultry workers. Therefore, a development of influenza H5N2 vaccines is desirable for pandemic preparation. In this study, we employed reverse genetics to generate a vaccine virus having HA and NA genes from A/Chicken/CY/A2628/2012 (E7, LPAI) and six internal genes from a Vero cell-adapted high-growth H5N1 vaccine virus (Vero-15). The reassortant H5N2 vaccine virus, E7-V15, presented high-growth efficiency in Vero cells (512 HAU, 107.6 TCID50/mL), and passed all tests for qualification of candidate vaccine viruses. In ferret immunization, two doses of inactivated whole virus antigens (3 μg of HA protein) adjuvanted with alum could induce robust antibody response (HI titre 113.14). In conclusion, we have established reverse genetics to generate a qualified reassortant H5N2 vaccine virus for further development. View Full-Text
Keywords: Pandemic preparedness; American-lineage reassortant influenza viruses; American-lineage H5N2 vaccine Pandemic preparedness; American-lineage reassortant influenza viruses; American-lineage H5N2 vaccine
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Chen, P.-L.; Hu, A. .-C.; Lin, C.-Y.; Weng, T.-C.; Lai, C.-C.; Tseng, Y.-F.; Cheng, M.-C.; Chia, M.-Y.; Lin, W.-C.; Yeh, C.-T.; Su, I.-J.; Lee, M.-S. Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness. Viruses 2019, 11, 543.

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