Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Cell Culture Media
2.2. Viruses and Virus Titrations
2.3. Antifoam Studies
2.4. Cell Density Experiments
2.4.1. Spin Tube Experiments
2.4.2. Bioreactor Experiments
2.5. Effect of Conditioned Culture Media on Virus Propagation
2.6. Batch Culture Experiments
2.7. Calculations
2.7.1. Specific Growth Rate: μ (h−1)
2.7.2. Cell Division Number (Cd):
2.8. Statistical Analysis
3. Results
3.1. Antifoam Studies
3.2. Cell Density Effects
3.3. Conditioned Media
3.4. Batch Culture Experiments
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cell Density | Media Exchange | Growth Rate μ (h−1) | Cell Division Number (cd) | |||||
---|---|---|---|---|---|---|---|---|
s | N | s | N | |||||
BHK200 | 1 × 106 cells/mL | +30% | 0.06 | 0.00 | 6 | 1.92 | 0.16 | 6 |
1 × 106 cells/mL | +100% | 0.06 | 0.01 | 6 | 2.05 | 0.18 | 6 | |
3 × 106 cells/mL | +30% | 0.03 | 0.00 | 6 | 0.87 | 0.12 | 6 | |
3 × 106 cells/mL | +100% | 0.04 | 0.00 | 6 | 1.36 | 0.12 | 6 | |
BHK300B | 1 × 106 cells/mL | +30% | 0.04 | 0.00 | 6 | 1.47 | 0.10 | 6 |
1 × 106 cells/mL | +100% | 0.04 | 0.00 | 6 | 1.54 | 0.07 | 6 | |
3 × 106 cells/mL | +30% | 0.02 | 0.00 | 6 | 0.83 | 0.16 | 6 | |
3 × 106 cells/mL | +100% | 0.03 | 0.00 | 6 | 1.13 | 0.10 | 6 | |
BHK300G | 1 × 106 cells/mL | +30% | 0.06 | 0.00 | 6 | 2.07 | 0.16 | 6 |
1 × 106 cells/mL | +100% | 0.06 | 0.00 | 6 | 2.02 | 0.17 | 6 | |
3 × 106 cells/mL | +30% | 0.03 | 0.01 | 6 | 0.94 | 0.17 | 6 | |
3 × 106 cells/mL | +100% | 0.04 | 0.00 | 4 | 1.40 | 0.07 | 4 |
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Dill, V.; Ehret, J.; Zimmer, A.; Beer, M.; Eschbaumer, M. Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus. Viruses 2019, 11, 511. https://doi.org/10.3390/v11060511
Dill V, Ehret J, Zimmer A, Beer M, Eschbaumer M. Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus. Viruses. 2019; 11(6):511. https://doi.org/10.3390/v11060511
Chicago/Turabian StyleDill, Veronika, Janike Ehret, Aline Zimmer, Martin Beer, and Michael Eschbaumer. 2019. "Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus" Viruses 11, no. 6: 511. https://doi.org/10.3390/v11060511