Next Article in Journal
The Utility of Data Transformation for Alignment, De Novo Assembly and Classification of Short Read Virus Sequences
Next Article in Special Issue
Unprecedented Diversity of Lactococcal Group 936 Bacteriophages Revealed by Amplicon Sequencing of the Portal Protein Gene
Previous Article in Journal
In Depth Breadth Analyses of Human Blockade Responses to Norovirus and Response to Vaccination
Previous Article in Special Issue
A Cryptic Non-Inducible Prophage Confers Phage-Immunity on the Streptococcus thermophilus M17PTZA496
Article Menu

Export Article

Open AccessArticle

Isolation and Characterization of Lactobacillus brevis Phages

1
School of Microbiology, University College of Cork, T12 YT20 Cork, Ireland
2
APC Microbiome Ireland, University College of Cork, T12 YT20 Cork, Ireland
3
Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, 43124, Parma, Italy
4
Department Microbiology and Biotechnology, Federal Research Centre of Nutrition and Food, Max Rubner-Institut, 24103, Kiel, Germany
5
Department Physiology Biochemistry and Immunology, Biomedical Research Institute, Hasselt University, B-3590 Diepenbeek, Belgium
6
HEINEKEN Global Innovation and Research, Heineken Supply Chain B.V, 2382 Zoeterwoude, The Netherlands
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(5), 393; https://doi.org/10.3390/v11050393
Received: 26 March 2019 / Revised: 23 April 2019 / Accepted: 24 April 2019 / Published: 26 April 2019
  |  
PDF [2800 KB, uploaded 8 May 2019]
  |  

Abstract

Lactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potential to control such spoilage bacteria. Just a few studies on phages infecting Lactobacillus brevis have been performed to date and in the present study, we report the isolation and characterization of five virulent phages capable of infecting Lb. brevis strains. The analysis reveals a high diversity among the isolates, with members belonging to both, the Myoviridae and Siphoviridae families. One isolate, designated phage 3-521, possesses a genome of 140.8 kb, thus representing the largest Lb. brevis phage genome sequenced to date. While the isolated phages do not propagate on Lb. brevis beer-spoiling strains, phages showed activity against these strains, impairing the growth of some Lb. brevis strains. The results highlight the potential of bacteriophage-based treatments as an effective approach to prevent bacterial spoilage of beer. View Full-Text
Keywords: Lactobacillus brevis; bacteriophage; virulent; beer; fermentation; genomic and proteomic analysis Lactobacillus brevis; bacteriophage; virulent; beer; fermentation; genomic and proteomic analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Feyereisen, M.; Mahony, J.; Lugli, G.A.; Ventura, M.; Neve, H.; Franz, C.M.A.P.; Noben, J.-P.; O’Sullivan, T.; van Sinderen, D. Isolation and Characterization of Lactobacillus brevis Phages. Viruses 2019, 11, 393.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top