Experimental Vertical Transmission of Chikungunya Virus by Brazilian and Florida Aedes Albopictus Populations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Mosquito Populations
2.3. Mosquito Rearing
2.4. Virus and Mosquito Oral Infection
2.5. Maintenance of Adults
2.6. Determination of Progeny Infectious Status
2.7. Statistical Analyses
3. Results
3.1. Parental Female Feeding and Oviposition
3.2. CHIKV Dissemination in Parental Females and Vertical Transmission
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mosquito Stages | Descriptive Statistics | Ae. albopictus Population | |
---|---|---|---|
Rio de Janeiro/BR | Okeechobee/US | ||
Eggs | Total | 5299 | 3384 |
Mean (SD) | 23.14 (25.80) | 26.23 (25.86) | |
Minimum–Maximum | 0–119 | 0–121 | |
Pupae | Total | 2818 | 1885 |
Mean (SD) | 20.13 (16.82) | 20.71 (20.85) | |
Minimum-Maximum | 0–86 | 1–107 | |
Adult females | Total | 1309 | 866 |
Mean (SD) | 9.49 (8.06) | 9.52 (9.75) | |
Minimum–Maximum | 0–43 | 0–46 | |
Positive/Total Female Pools | 1/138 | 0/94 | |
Minimum Infection Rate | 0.76 | 0 | |
Adult males | Total | 1399 | 942 |
Mean (SD) | 10.14 (8.67) | 10.35 (10.76) | |
Minimum–Maximum | 0–38 | 0–60 | |
Positive/Total Male Pools | 4/147 | 1/102 | |
Minimum Infection Rate | 2.86 | 1.06 |
Response | Model | Effect | Estimate | Standard Error | 95% CI | |
---|---|---|---|---|---|---|
Lower Bound | Upper Bound | |||||
Viral dissemination (parental females) | #1 | (Intercept) | 9.125 | 0.263 | 5.625 | 15.904 |
Population origin: USA | 0.500 | 0.350 | 0.248 | 0.987 | ||
#2 | (Intercept) | 6.312 | 0.122 | 5.007 | 8.067 | |
Vertical transmission: positive | 2.376 | 1.566 | NA | NA | ||
Viral titer (parental females) | #3 | (Intercept) | 12.607 | 0.426 | 11.773 | 13.441 |
Population origin: USA | −2.913 | 0.642 | −4.172 | −1.654 | ||
#4 | (Intercept) | 11.332 | 0.334 | 10.678 | 11.987 | |
Vertical transmission: positive | −0.143 | 2.274 | −4.599 | 4.314 | ||
Viral titer (progeny) | #5 | (Intercept) | 1.306 | 0.033 | 1.241 | 1.372 |
Population origin: USA | −0.006 | 0.053 | −0.111 | 0.099 | ||
#6 | (Intercept) | 2.462 | 0.099 | 2.268 | 2.656 | |
Vertical transmission: positive | −0.580 | 0.886 | −2.316 | 1.155 |
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Honório, N.A.; Wiggins, K.; Eastmond, B.; Câmara, D.C.P.; Alto, B.W. Experimental Vertical Transmission of Chikungunya Virus by Brazilian and Florida Aedes Albopictus Populations. Viruses 2019, 11, 353. https://doi.org/10.3390/v11040353
Honório NA, Wiggins K, Eastmond B, Câmara DCP, Alto BW. Experimental Vertical Transmission of Chikungunya Virus by Brazilian and Florida Aedes Albopictus Populations. Viruses. 2019; 11(4):353. https://doi.org/10.3390/v11040353
Chicago/Turabian StyleHonório, Nildimar Alves, Keenan Wiggins, Bradley Eastmond, Daniel Cardoso Portela Câmara, and Barry W. Alto. 2019. "Experimental Vertical Transmission of Chikungunya Virus by Brazilian and Florida Aedes Albopictus Populations" Viruses 11, no. 4: 353. https://doi.org/10.3390/v11040353