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Viruses 2019, 11(4), 327; https://doi.org/10.3390/v11040327

Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela

1
UCLouvain, Earth and Life Institute, Applied Microbiology-Phytopathology, Croix du Sud 2-L07.05.03, 1348 Louvain-la-Neuve, Belgium
2
La Universidad del Zulia (LUZ), Unidad Técnica Fitosanitaria, Maracaibo 4005, Estado Zulia, Venezuela
3
Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí, Manabí 130105, Ecuador
4
UCLouvain, Earth and Life Institute, Applied Microbiology-Laboratory of Food and Environmental Microbiology, Croix du Sud 2-L7.05.12, 1348 Louvain-la-Neuve, Belgium
*
Authors to whom correspondence should be addressed.
Present address: Section of Microbiology and Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, London SW72AZ, UK.
Received: 21 February 2019 / Revised: 22 March 2019 / Accepted: 2 April 2019 / Published: 4 April 2019
(This article belongs to the Special Issue Plant Virus Ecology and Biodiversity)
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Abstract

Begomoviruses are one of the major groups of plant viruses with an important economic impact on crop production in tropical and subtropical regions. The global spread of its polyphagous vector, the whitefly Bemisia tabaci, has contributed to the emergence and diversification of species within this genus. In this study, we found a putative novel begomovirus infecting tomato plants in Venezuela without a cognate DNA-B component. This begomovirus was genetically characterized and compared with related species. Furthermore, its infectivity was demonstrated by agroinoculation of infectious clones in tomato (Solanum lycopersicum) and Nicotiana benthamiana plants. The name Tomato twisted leaf virus (ToTLV) is proposed. ToTLV showed the typical genome organization of the DNA-A component of New World bipartite begomoviruses. However, the single DNA component of ToTLV was able to develop systemic infection in tomato and N. benthamiana plants, suggesting a monopartite nature of its genome. Interestingly, an additional open reading frame ORF was observed in ToTLV encompassing the intergenic region and the coat protein gene, which is not present in other closely related begomoviruses. A putative transcript from this region was amplified by strand-specific reverse transcription-PCR. Along with recent studies, our results showed that the diversity of monopartite begomoviruses from the New World is greater than previously thought. View Full-Text
Keywords: begomovirus evolution; Geminiviridae; Solanum lycopersicum; tomato crop begomovirus evolution; Geminiviridae; Solanum lycopersicum; tomato crop
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Romay, G.; Geraud-Pouey, F.; Chirinos, D.T.; Mahillon, M.; Gillis, A.; Mahillon, J.; Bragard, C. Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela. Viruses 2019, 11, 327.

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