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Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB)

1
Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA
2
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA
3
Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA
*
Authors to whom correspondence should be addressed.
Present address: Johns Hopkins Applied Physics Laboratory Laurel, MD 20723, USA.
Viruses 2019, 11(4), 307; https://doi.org/10.3390/v11040307
Received: 27 February 2019 / Revised: 22 March 2019 / Accepted: 25 March 2019 / Published: 29 March 2019
(This article belongs to the Special Issue Porcine Viruses 2019)
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PDF [2016 KB, uploaded 29 March 2019]
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Abstract

Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune response and is critical for virulence in swine. Using the yeast two-hybrid system, we identified protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a specific binding host partner for E2. We further confirmed the occurrence of this interaction in CSFV-infected swine cells by using two independent methodologies: Co-immunoprecipitation and Proximity Ligation Assay. In addition, we demonstrated that pharmacological activation of the PP1 pathway has a negative effect on CSFV replication while inhibition of the PP1 pathway or knockdown of PPP1CB by siRNA had no observed effect. Overall, our data suggests that the CSFV E2 and PPP1CB protein interact in infected cells, and that activation of the PP1 pathway decreases virus replication. View Full-Text
Keywords: E2; classical swine fever virus; CSFV; Viral-Host protein interactions E2; classical swine fever virus; CSFV; Viral-Host protein interactions
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Vuono, E.A.; Ramirez-Medina, E.; Holinka, L.G.; Baker-Branstetter, R.; Borca, M.V.; Gladue, D.P. Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB). Viruses 2019, 11, 307.

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