A Single V672F Substitution in the Spike Protein of Field-Isolated PEDV Promotes Cell–Cell Fusion and Replication in VeroE6 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Plasmid Constructs
2.3. Syncytium Formation Assay
2.4. Recovery of Recombinant PEDV from Infectious cDNA Clones
2.5. Immunofluorescence Assay
2.6. Flow Cytometry
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. Distinct Cell–Cell Fusion Induced by SYN144 and SG2 in VeroE6-APN Cells
3.2. SG2 Lacking the C-Terminal Motifs Showed Strong Cell Surface Expression
3.3. Chimeric SYN144 Bearing RBD and S1/S2 Cleavage of SG2 Failed to Induce Syncytium Formation
3.4. Amino Acids in the RBD Are Critical for Cell–Cell Fusion Induced by SYN144
3.5. The Effect of the V672F Substitution Is Specific for VeroE6 Cells
3.6. Effect of the V672F Substitution on the Recombinant PEDV Growth
4. Discussion
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Wanitchang, A.; Saenboonrueng, J.; Kaewborisuth, C.; Srisutthisamphan, K.; Jongkaewwattana, A. A Single V672F Substitution in the Spike Protein of Field-Isolated PEDV Promotes Cell–Cell Fusion and Replication in VeroE6 Cells. Viruses 2019, 11, 282. https://doi.org/10.3390/v11030282
Wanitchang A, Saenboonrueng J, Kaewborisuth C, Srisutthisamphan K, Jongkaewwattana A. A Single V672F Substitution in the Spike Protein of Field-Isolated PEDV Promotes Cell–Cell Fusion and Replication in VeroE6 Cells. Viruses. 2019; 11(3):282. https://doi.org/10.3390/v11030282
Chicago/Turabian StyleWanitchang, Asawin, Janya Saenboonrueng, Challika Kaewborisuth, Kanjana Srisutthisamphan, and Anan Jongkaewwattana. 2019. "A Single V672F Substitution in the Spike Protein of Field-Isolated PEDV Promotes Cell–Cell Fusion and Replication in VeroE6 Cells" Viruses 11, no. 3: 282. https://doi.org/10.3390/v11030282
APA StyleWanitchang, A., Saenboonrueng, J., Kaewborisuth, C., Srisutthisamphan, K., & Jongkaewwattana, A. (2019). A Single V672F Substitution in the Spike Protein of Field-Isolated PEDV Promotes Cell–Cell Fusion and Replication in VeroE6 Cells. Viruses, 11(3), 282. https://doi.org/10.3390/v11030282