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Viruses 2019, 11(2), 112; https://doi.org/10.3390/v11020112

Piscine Orthoreovirus 3 Is Not the Causative Pathogen of Proliferative Darkening Syndrome (PDS) of Brown Trout (Salmo trutta fario)

1
Institute for Infectious Diseases and Zoonoses, Department for Veterinary Sciences, LMU Munich, 80539 Munich, Germany
2
Bavarian Environment Agency, Unit Aquatic Toxicology, Pathology, 82407 Wielenbach, Germany
3
Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, 20246 Hamburg, Germany
4
Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 20251 Hamburg, Germany
5
Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität Würzburg, 97078 Würzburg, Germany
6
Fish Disease Research Unit, Institute for Parasitology, University of Veterinary Medicine, 30559 Hannover, Germany
*
Author to whom correspondence should be addressed.
Received: 10 January 2019 / Revised: 18 January 2019 / Accepted: 25 January 2019 / Published: 28 January 2019
(This article belongs to the Section Animal Viruses)
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Abstract

The proliferative darkening syndrome (PDS) is a lethal disease of brown trout (Salmo trutta fario) which occurs in several alpine Bavarian limestone rivers. Because mortality can reach 100%, PDS is a serious threat for affected fish populations. Recently, Kuehn and colleagues reported that a high throughput RNA sequencing approach identified a piscine orthoreovirus (PRV) as a causative agent of PDS. We investigated samples from PDS-affected fish obtained from two exposure experiments performed at the river Iller in 2008 and 2009. Using a RT-qPCR and a well-established next-generation RNA sequencing pipeline for pathogen detection, PRV-specific RNA was not detectable in PDS fish from 2009. In contrast, PRV RNA was readily detectable in several organs from diseased fish in 2008. However, similar virus loads were detectable in the control fish which were not exposed to Iller water and did not show any signs of the disease. Therefore, we conclude that PRV is not the causative agent of PDS of brown trout in the rhithral region of alpine Bavarian limestone rivers. The abovementioned study by Kuehn used only samples from the exposure experiment from 2008 and detected a subclinical PRV bystander infection. Work is ongoing to identify the causative agent of PDS. View Full-Text
Keywords: proliferative darkening syndrome; black trout syndrome; piscine orthoreovirus; orthoreovirus; brown trout; Salmo trutta fario; next generation sequencing; RT-qPCR proliferative darkening syndrome; black trout syndrome; piscine orthoreovirus; orthoreovirus; brown trout; Salmo trutta fario; next generation sequencing; RT-qPCR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Fux, R.; Arndt, D.; Langenmayer, M.C.; Schwaiger, J.; Ferling, H.; Fischer, N.; Indenbirken, D.; Grundhoff, A.; Dölken, L.; Adamek, M.; Steinhagen, D.; Sutter, G. Piscine Orthoreovirus 3 Is Not the Causative Pathogen of Proliferative Darkening Syndrome (PDS) of Brown Trout (Salmo trutta fario). Viruses 2019, 11, 112.

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