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Open AccessArticle

A Novel Genus of Actinobacterial Tectiviridae

1
Department of Biological Sciences, University of Maryland Baltimore County (UMBC), Baltimore, MD 21250, USA
2
Keith R. Porter Imaging Facility, University of Maryland Baltimore County (UMBC), Baltimore, MD 21250, USA
3
College of Natural and Mathematical Sciences, University of Maryland Baltimore County (UMBC), Baltimore, MD 21250, USA
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(12), 1134; https://doi.org/10.3390/v11121134
Received: 27 October 2019 / Revised: 3 December 2019 / Accepted: 4 December 2019 / Published: 7 December 2019
(This article belongs to the Section Bacterial Viruses)
Streptomyces phages WheeHeim and Forthebois are two novel members of the Tectiviridae family. These phages were isolated on cultures of the plant pathogen Streptomyces scabiei, known for its worldwide economic impact on potato crops. Transmission electron microscopy showed viral particles with double-layered icosahedral capsids, and frequent instances of protruding nanotubes harboring a collar-like structure. Mass-spectrometry confirmed the presence of lipids in the virion, and serial purification of colonies from turbid plaques and immunity testing revealed that both phages are temperate. Streptomyces phages WheeHeim and Forthebois have linear dsDNA chromosomes (18,266 bp and 18,251 bp long, respectively) with the characteristic two-segment architecture of the Tectiviridae. Both genomes encode homologs of the canonical tectiviral proteins (major capsid protein, packaging ATPase and DNA polymerase), as well as PRD1-type virion-associated transglycosylase and membrane DNA delivery proteins. Comparative genomics and phylogenetic analyses firmly establish that these two phages, together with Rhodococcus phage Toil, form a new genus within the Tectiviridae, which we have tentatively named Deltatectivirus. The identification of a cohesive clade of Actinobacteria-infecting tectiviruses with conserved genome structure but with scant sequence similarity to members of other tectiviral genera confirms that the Tectiviridae are an ancient lineage infecting a broad range of bacterial hosts. View Full-Text
Keywords: tectivirus; Streptomyces; actinobacteria; pathogen; plant; lipid membrane; capsid protein; potato scab tectivirus; Streptomyces; actinobacteria; pathogen; plant; lipid membrane; capsid protein; potato scab
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MDPI and ACS Style

Caruso, S.M.; deCarvalho, T.N.; Huynh, A.; Morcos, G.; Kuo, N.; Parsa, S.; Erill, I. A Novel Genus of Actinobacterial Tectiviridae. Viruses 2019, 11, 1134.

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