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Next-Generation Sequencing on Insectivorous Bat Guano: An Accurate Tool to Identify Arthropod Viruses of Potential Agricultural Concern

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Animal Santé Territoire Risque Environnement- Unité Mixe de Recherche 117 (ASTRE) Univ. Montpellier, Centre International de Recherche Agronomique pour le Développement (CIRAD), Institut National de la Recherche Agronomique, 34398 Montpellier, France
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Centre International de Recherche Agronomique pour le Développement (CIRAD), Research Platform-Production and Conservation in Partership, Unité Mixe de Recherche ASTRE, Harare, Zimbabwe
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Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle- Unité Mixe de Recherche 224 (MIVEGEC), Institut de Recherche pour le Développement (IRD), Centre National de Recherche Scientifique (CNRS), Univ. Montpellier, 34398 Montpellier, France
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Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP167, Mt. Pleasant Harare P.O. Box MP167, Zimbabwe
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Université de Liège, Laboratoire de Génétique de la Conservation, GeCoLAB, 4000 Liège, Belgium
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Institut des Sciences de l’Evolution de Montpellier (ISEM), Univ Montpellier, Centre National de Recherche Scientifique (CNRS), Ecole Pratique des Hautes Etude (EPHE)s, Institut de Recherche pour le Développement (IRD), 34398 Montpellier, France
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Montpellier GenomiX (MGX), Biocampus Montpellier, Centre National de Recherche Scientifique (CNRS), Intitut National de la Santé et de la Recherche Médicale (INSERM), Univ Montpellier, 34094 Montpellier, France
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Institut des Sciences de l’Evolution de Montpellier (ISEM) Univ. Montpellier, Centre National de Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Centre International de Recherche Agronomique pour le Développement (CIRAD), 34000 Montpellier, France
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Author to whom correspondence should be addressed.
Viruses 2019, 11(12), 1102; https://doi.org/10.3390/v11121102
Received: 12 October 2019 / Revised: 12 November 2019 / Accepted: 26 November 2019 / Published: 28 November 2019
(This article belongs to the Section Animal Viruses)
Viruses belonging to the Dicistroviridae family have attracted a great deal of attention from scientists owing to their negative impact on agricultural economics, as well as their recent identification as potential aetiological agents of febrile illness in human patients. On the other hand, some Dicistroviruses are also studied for their potential biopesticide properties. To date, Dicistrovirus characterized in African mainland remain scarce. By using High-Throughput Sequencing technology on insectivorous bat faeces (Hipposideros Caffer) sampled in a cave used by humans to collect bat guano (bat manure) as fertilizer in Zimbabwe, we characterized the full-length sequences of three Dicistrovirus belonging to the Cripavirus and Aparavirus genus: Big Sioux River Virus-Like (BSRV-Like), Acute Bee Paralysis Virus (ABPV), and Aphid Lethal Paralysis Virus (ALPV). Phylogenetic analyses of ORF-1 and ORF-2 genes showed a complex evolutionary history between BSRV and close viruses, as well as for the Aparavirus genus. Herewith, we provide the first evidence of the presence of Dicistrovirus in Zimbabwe and highlight the need to further document the impact of such viruses on crops, as well as in beekeeping activities in Zimbabwe which represent a crucial source of income for Zimbabwean people. View Full-Text
Keywords: Dicistrovirus; Bats; Faeces; Phylogeny; HTS; Zimbabwe Dicistrovirus; Bats; Faeces; Phylogeny; HTS; Zimbabwe
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Mathieu, B.; Valérie, N.; Davies, P.; Johan, M.; Adrien, A.; Pierre, B.; Frédérique, C.; Célia, B.; Vanina, B.; Loïc, T.; Gift, M.; Dorothée, M.; Serge, M.; Florian, L. Next-Generation Sequencing on Insectivorous Bat Guano: An Accurate Tool to Identify Arthropod Viruses of Potential Agricultural Concern. Viruses 2019, 11, 1102.

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