Next Article in Journal
Multiple Correspondence Analysis on Amino Acid Properties within the Variable Region of the Capsid Protein Shows Differences between Classical and Virulent Systemic Feline Calicivirus Strains
Previous Article in Journal
Identification of a Novel Adélie Penguin Circovirus at Cape Crozier (Ross Island, Antarctica)
Previous Article in Special Issue
Characterization of Klebsiella pneumoniae ST11 Isolates and Their Interactions with Lytic Phages
Open AccessArticle

Pseudomonas aeruginosa PA5oct Jumbo Phage Impacts Planktonic and Biofilm Population and Reduces Its Host Virulence

1
Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, 51-148 Wroclaw, Poland
2
Laboratory of Gene Technology, KU Leuven, 3001 Heverlee, Belgium
3
Department of Biochemistry and Genetics, Institute of Biology, The Jan Kochanowski University in Kielce, 25-406 Kielce, Poland
4
Department of Molecular Physics, Institute of Physics, The Jan Kochanowski University in Kielce, 25-406 Kielce, Poland
5
Laboratory of Computational Systems Biology, KU Leuven, 3000 Leuven, Belgium
6
National Children Research Centre, Our Lady’s Children’s Hospital, Crumlin, 12 Dublin, Ireland
7
Department of Molecular Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, 9 Dublin, Ireland
*
Author to whom correspondence should be addressed.
Current address: Department of Microbiology, Ohio State University, Columbus, 43210 OH, USA.
Viruses 2019, 11(12), 1089; https://doi.org/10.3390/v11121089
Received: 25 September 2019 / Revised: 19 November 2019 / Accepted: 20 November 2019 / Published: 23 November 2019
(This article belongs to the Special Issue Bacteriophages and Biofilms)
The emergence of phage-resistant mutants is a key aspect of lytic phages-bacteria interaction and the main driver for the co-evolution between both organisms. Here, we analyze the impact of PA5oct jumbo phage treatment on planktonic/cell line associated and sessile P. aeruginosa population. Besides its broad-spectrum activity and efficient bacteria reduction in both airway surface liquid (ASL) model, and biofilm matrix degradation, PA5oct appears to persist in most of phage-resistant clones. Indeed, a high percentage of resistance (20/30 clones) to PA5oct is accompanied by the presence of phage DNA within bacterial culture. Moreover, the maintenance of this phage in the bacterial population correlates with reduced P. aeruginosa virulence, coupled with a sensitization to innate immune mechanisms, and a significantly reduced growth rate. We observed rather unusual consequences of PA5oct infection causing an increased inflammatory response of monocytes to P. aeruginosa. This phenomenon, combined with the loss or modification of the phage receptor, makes most of the phage-resistant clones significantly less pathogenic in in vivo model. These findings provide new insights into the general knowledge of giant phages biology and the impact of their application in phage therapy. View Full-Text
Keywords: giant bacteriophage; Pseudomonas aeruginosa; biofilm; Airway Surface Liquid Infection model; phage-resistant mutants giant bacteriophage; Pseudomonas aeruginosa; biofilm; Airway Surface Liquid Infection model; phage-resistant mutants
Show Figures

Figure 1

MDPI and ACS Style

Olszak, T.; Danis-Wlodarczyk, K.; Arabski, M.; Gula, G.; Maciejewska, B.; Wasik, S.; Lood, C.; Higgins, G.; Harvey, B.J.; Lavigne, R.; Drulis-Kawa, Z. Pseudomonas aeruginosa PA5oct Jumbo Phage Impacts Planktonic and Biofilm Population and Reduces Its Host Virulence. Viruses 2019, 11, 1089.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop