Next Article in Journal
Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats
Previous Article in Journal
Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus
Open AccessArticle

Large Phenotypic and Genetic Diversity of Prophages Induced from the Fish Pathogen Vibrio anguillarum

Marine Biological Section, University of Copenhagen, Strandpromenaden 5, DK-3000 Helsingør, Denmark
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(11), 983; https://doi.org/10.3390/v11110983
Received: 3 October 2019 / Accepted: 15 October 2019 / Published: 24 October 2019
(This article belongs to the Section Bacterial Viruses)
Vibrio anguillarum is a marine pathogenic bacterium that causes vibriosis in fish and shellfish. Although prophage-like sequences have been predicted in V. anguillarum strains, many are not characterized, and it is not known if they retain the functional capacity to form infectious particles that can infect and lysogenize other bacterial hosts. In this study, the genome sequences of 28 V. anguillarum strains revealed 55 different prophage-related elements. Chemical and spontaneous induction allowed a collection of 42 phage isolates, which were classified in seven different groups according to a multiplex PCR assay. One shared prophage sequence, p41 (group III), was present in 17 V. anguillarum strains, suggesting that this specific element is very dynamically exchanged among V. anguillarum populations. Interestingly, the host range of genetically identical phages was highly dependent on the strains used for proliferation, indicating that phenotypic properties of phages were partly regulated by the host. Finally, experimental evidence displayed that the induced phage ɸVa_90-11-287_p41 was able to lysogenize V. anguillarum strain Ba35, and subsequently spontaneously become released from the lysogenized cells, demonstrating an efficient transfer of the phage among V. anguillarum strains. Altogether, the results showed large genetic and functional diversity and broad distribution of prophages in V. anguillarum, and demonstrated the potential of prophages as drivers of evolution in V. anguillarum strains. View Full-Text
Keywords: Vibrio; prophage; genomics; lysogenic conversion; virulence; aquaculture; H20-like phages Vibrio; prophage; genomics; lysogenic conversion; virulence; aquaculture; H20-like phages
Show Figures

Figure 1

MDPI and ACS Style

Castillo, D.; Andersen, N.; Kalatzis, P.G.; Middelboe, M. Large Phenotypic and Genetic Diversity of Prophages Induced from the Fish Pathogen Vibrio anguillarum. Viruses 2019, 11, 983.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop