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Open AccessArticle

Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre, Czech Academy of Sciences, 370 05 České Budějovice, Czech Republic
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Viruses 2019, 11(11), 982; https://doi.org/10.3390/v11110982
Received: 18 September 2019 / Revised: 21 October 2019 / Accepted: 23 October 2019 / Published: 24 October 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects F. ananassa and F. vesca plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single F. ananassa host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via Aphis fabae and A. ruborum, further mechanically transmitted to Nicotiana occidentalis 37B and sub-inoculated to N. benthamiana, N. benthamiana DCL2/4i, N. occidentalis 37B, and Physalis floridana plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed F. vesca clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus Cytorhabdovirus. Acyrthosiphon malvae, A. fabae, and A. ruborum were shown to transmit StrV-1 under experimental conditions. View Full-Text
Keywords: novel cytorhabdovirus; high-throughput sequencing; aphid transmission; mechanical inoculation; grafting-mediated virus transmission; Fragaria spp.; Chaetosiphon fragaefolii novel cytorhabdovirus; high-throughput sequencing; aphid transmission; mechanical inoculation; grafting-mediated virus transmission; Fragaria spp.; Chaetosiphon fragaefolii
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MDPI and ACS Style

Fránová, J.; Přibylová, J.; Koloniuk, I. Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus. Viruses 2019, 11, 982.

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