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Open AccessArticle

PCV2 Regulates Cellular Inflammatory Responses through Dysregulating Cellular miRNA-mRNA Networks

by Chang Li 1,2,3, Yumei Sun 3, Jing Li 1,3, Changsheng Jiang 1,3, Wei Zeng 1,3, Hao Zhang 1,3, Shengxian Fan 2,3 and Qigai He 1,2,3,*
1
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430000, China
2
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430000, China
3
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430000, China
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(11), 1055; https://doi.org/10.3390/v11111055
Received: 4 October 2019 / Revised: 31 October 2019 / Accepted: 1 November 2019 / Published: 13 November 2019
(This article belongs to the Section Animal Viruses)
Porcine circovirus type 2 (PCV2) is closely linked to postweaning multisystemic wasting syndrome (PMWS) and other PCV-associated diseases (PCVADs), which influence the global pig industry. MicroRNAs (miRNAs) are evolutionarily conserved classes of endogenous small non-coding RNA that regulate almost every cellular process. According to our previous transcription study, PCV2 infection causes up-regulation of genes related to inflammation. To reveal the function of miRNAs in PCV2 infection and PCV2-encoded miRNAs, next generation sequencing and data analysis was performed to explore miRNA expression in PCV2-infected cells and non-infected cells. Data analysis found some small RNAs matched the PCV2 genome but PCV2 does not express miRNAs in an in vitro infection (PK-15 cells). More than 297 known and 427 novel miRNAs were identified, of which 44 miRNAs were differently expressed (DE). The pathways of inflammation mediated by chemokine and cytokine signaling pathway (P00031), were more perturbed in PCV2-infected cells than in mock controls. DE miRNAs and DE mRNA interaction network clearly revealed that PCV2 regulates the cellular inflammatory response through dysregulating the cellular miRNA-mRNA network. MiRNA overexpression and down-expression results demonstrated that miRNA dysregulation could affect PCV2 infection-induced cellular inflammatory responses. Our study revealed that host miRNA can be dysregulated by PCV2 infection and play an important role in PCV2-modulated inflammation. View Full-Text
Keywords: porcine circovirus type 2; porcine kidney 15 cell line; microRNAs; interaction network; inflammation porcine circovirus type 2; porcine kidney 15 cell line; microRNAs; interaction network; inflammation
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MDPI and ACS Style

Li, C.; Sun, Y.; Li, J.; Jiang, C.; Zeng, W.; Zhang, H.; Fan, S.; He, Q. PCV2 Regulates Cellular Inflammatory Responses through Dysregulating Cellular miRNA-mRNA Networks. Viruses 2019, 11, 1055.

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