Next Article in Journal
Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil
Previous Article in Journal
Viromics Reveal a Number of Novel RNA Viruses in Swedish Mosquitoes
Open AccessArticle

A Fosmid-Based System for the Generation of Recombinant Cercopithecine Alphaherpesvirus 2 Encoding Reporter Genes

1
Infection Biology Unit, German Primate Center-Leibniz Institute for Primate Research, 37077 Göttingen, Germany
2
Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
3
Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, 37077 Göttingen, Germany
4
German Center for Cardiovascular Research (DZHK), partner site, 37099 Göttingen, Germany
5
Institute of Virology, Hannover Medical School, 30625 Hannover, Germany
6
Faculty of Biology and Psychology, University Göttingen, 37073 Göttingen, Germany
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(11), 1026; https://doi.org/10.3390/v11111026
Received: 23 September 2019 / Revised: 30 October 2019 / Accepted: 31 October 2019 / Published: 5 November 2019
(This article belongs to the Section Animal Viruses)
The transmission of Macacine alphaherpesvirus 1 (McHV-1) from macaques, the natural host, to humans causes encephalitis. In contrast, human infection with Cercopithecine alphaherpesvirus 2 (CeHV-2), a closely related alphaherpesvirus from African vervet monkeys and baboons, has not been reported and it is believed that CeHV-2 is apathogenic in humans. The reasons for the differential neurovirulence of McHV-1 and CeHV-2 have not been explored on a molecular level, in part due to the absence of systems for the production of recombinant viruses. Here, we report the generation of a fosmid-based system for rescue of recombinant CeHV-2. Moreover, we show that, in this system, recombineering can be used to equip CeHV-2 with reporter genes. The recombinant CeHV-2 viruses replicated with the same efficiency as uncloned, wt virus and allowed the identification of cell lines that are highly susceptible to CeHV-2 infection. Collectively, we report a system that allows rescue and genetic modification of CeHV-2 and likely other alphaherpesviruses. This system should aid future analysis of CeHV-2 biology. View Full-Text
Keywords: Cercopithecine alphaherpesvirus 2; fosmid; recombineering Cercopithecine alphaherpesvirus 2; fosmid; recombineering
Show Figures

Figure 1

MDPI and ACS Style

Chukhno, E.; Gärtner, S.; Rahman Siregar, A.; Mehr, A.; Wende, M.; Petkov, S.; Götting, J.; Dhingra, A.; Schulz, T.; Pöhlmann, S.; Winkler, M. A Fosmid-Based System for the Generation of Recombinant Cercopithecine Alphaherpesvirus 2 Encoding Reporter Genes. Viruses 2019, 11, 1026.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop