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Open AccessArticle

Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

1
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark
2
The Natural History Museum of Denmark, University of Copenhagen, 2100 Copenhagen, Denmark
3
School of Animal Science, Xichang College, Xichang 615013, China
4
Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(9), 486; https://doi.org/10.3390/v10090486
Received: 3 August 2018 / Revised: 3 September 2018 / Accepted: 10 September 2018 / Published: 11 September 2018
(This article belongs to the Special Issue Viruses and Bats 2019)
Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations. View Full-Text
Keywords: Vespertilionidae; coronavirus; nucleotide sequencing; phylogenetic analysis; Europe; host restriction Vespertilionidae; coronavirus; nucleotide sequencing; phylogenetic analysis; Europe; host restriction
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Lazov, C.M.; Chriél, M.; Baagøe, H.J.; Fjederholt, E.; Deng, Y.; Kooi, E.A.; Belsham, G.J.; Bøtner, A.; Rasmussen, T.B. Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark. Viruses 2018, 10, 486.

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