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Phage Genetic Engineering Using CRISPR–Cas Systems

Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA
Viruses 2018, 10(6), 335;
Received: 30 April 2018 / Revised: 17 June 2018 / Accepted: 17 June 2018 / Published: 19 June 2018
(This article belongs to the Special Issue Applications of CRISPR Technology in Virology 2018)
Since their discovery over a decade ago, the class of prokaryotic immune systems known as CRISPR–Cas have afforded a suite of genetic tools that have revolutionized research in model organisms spanning all domains of life. CRISPR-mediated tools have also emerged for the natural targets of CRISPR–Cas immunity, the viruses that specifically infect bacteria, or phages. Despite their status as the most abundant biological entities on the planet, the majority of phage genes have unassigned functions. This reality underscores the need for robust genetic tools to study them. Recent reports have demonstrated that CRISPR–Cas systems, specifically the three major types (I, II, and III), can be harnessed to genetically engineer phages that infect diverse hosts. Here, the mechanisms of each of these systems, specific strategies used, and phage editing efficacies will be reviewed. Due to the relatively wide distribution of CRISPR–Cas systems across bacteria and archaea, it is anticipated that these immune systems will provide generally applicable tools that will advance the mechanistic understanding of prokaryotic viruses and accelerate the development of novel technologies based on these ubiquitous organisms. View Full-Text
Keywords: CRISPR–Cas; bacteriophage; genome editing; phage genetics CRISPR–Cas; bacteriophage; genome editing; phage genetics
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Hatoum-Aslan, A. Phage Genetic Engineering Using CRISPR–Cas Systems. Viruses 2018, 10, 335.

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