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Viruses 2018, 10(6), 322; https://doi.org/10.3390/v10060322

Self-Assembled Nanoporous Biofilms from Functionalized Nanofibrous M13 Bacteriophage

1
Research Center for Energy Convergence and Technology Division, Pusan National University, Busan 46241, Korea
2
Department of Nano Fusion Technology, Pusan National University, Busan 46241, Korea
3
BK21 Plus Nanoconvergence Technology Division, Pusan National University, Busan 46241, Korea
4
Department of Chemistry, Pukyong National University, Busan 48513, Korea
5
Department of Nanoenergy Engineering, Pusan National University, Busan 46241, Korea
*
Author to whom correspondence should be addressed.
Received: 7 May 2018 / Revised: 8 June 2018 / Accepted: 12 June 2018 / Published: 12 June 2018
(This article belongs to the Special Issue Biotechnological Applications of Phage and Phage-Derived Proteins)
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Abstract

Highly periodic and uniform nanostructures, based on a genetically engineered M13 bacteriophage, displayed unique properties at the nanoscale that have the potential for a variety of applications. In this work, we report a multilayer biofilm with self-assembled nanoporous surfaces involving a nanofiber-like genetically engineered 4E-type M13 bacteriophage, which was fabricated using a simple pulling method. The nanoporous surfaces were effectively formed by using the networking-like structural layers of the M13 bacteriophage during self-assembly. Therefore, an external template was not required. The actual M13 bacteriophage-based fabricated multilayered biofilm with porous nanostructures agreed well with experimental and simulation results. Pores formed in the final layer had a diameter of about 150–500 nm and a depth of about 15–30 nm. We outline a filter application for this multilayered biofilm that enables selected ions to be extracted from a sodium chloride solution. Here, we describe a simple, environmentally friendly, and inexpensive fabrication approach with large-scale production potential. The technique and the multi-layered biofilms produced may be applied to sensor, filter, plasmonics, and bio-mimetic fields. View Full-Text
Keywords: M13 bacteriophage; biofilm; porous structure; filters; self-assembly M13 bacteriophage; biofilm; porous structure; filters; self-assembly
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Devaraj, V.; Han, J.; Kim, C.; Kang, Y.-C.; Oh, J.-W. Self-Assembled Nanoporous Biofilms from Functionalized Nanofibrous M13 Bacteriophage. Viruses 2018, 10, 322.

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