Live-Cell Imaging of Early Steps of Single HIV-1 Infection
1
Department of Pediatrics, Emory University, Atlanta, GA 30322, USA
2
Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(5), 275; https://doi.org/10.3390/v10050275
Received: 25 April 2018 / Revised: 15 May 2018 / Accepted: 18 May 2018 / Published: 19 May 2018
(This article belongs to the Special Issue Application of Advanced Imaging to the Study of Virus Replication)
Abstract
Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection. View Full-TextKeywords:
Single HIV imaging; viral fusion; uncoating; maturation; nuclear import; infection
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MDPI and ACS Style
Francis, A.C.; Melikyan, G.B. Live-Cell Imaging of Early Steps of Single HIV-1 Infection. Viruses 2018, 10, 275.
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