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Live-Cell Imaging of Early Steps of Single HIV-1 Infection

Department of Pediatrics, Emory University, Atlanta, GA 30322, USA
Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA
Author to whom correspondence should be addressed.
Viruses 2018, 10(5), 275;
Received: 25 April 2018 / Revised: 15 May 2018 / Accepted: 18 May 2018 / Published: 19 May 2018
(This article belongs to the Special Issue Application of Advanced Imaging to the Study of Virus Replication)
PDF [1720 KB, uploaded 19 May 2018]


Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection. View Full-Text
Keywords: Single HIV imaging; viral fusion; uncoating; maturation; nuclear import; infection Single HIV imaging; viral fusion; uncoating; maturation; nuclear import; infection

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Francis, A.C.; Melikyan, G.B. Live-Cell Imaging of Early Steps of Single HIV-1 Infection. Viruses 2018, 10, 275.

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