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Open AccessCommunication

Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting

1
The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UK
2
Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases, Binzhou 256600, Shandong, China
*
Authors to whom correspondence should be addressed.
Viruses 2018, 10(5), 242; https://doi.org/10.3390/v10050242
Received: 27 March 2018 / Revised: 30 April 2018 / Accepted: 3 May 2018 / Published: 5 May 2018
Several studies have shown that microRNA-targeting is an effective strategy for the selective control of tissue-tropism and pathogenesis of both DNA and RNA viruses. However, the exploitation of microRNA-targeting for the inhibition of transformation by oncogenic viruses has not been studied. The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transforming primary chicken spleen and bone marrow cells. Here, by engineering the target sequence of endogenous microRNA miR-142 downstream of the v-rel gene in a Replication-Competent ALV (avian leukosis virus) long terminal repeat (LTR) with a splice acceptor (RCAS) vector and using a v-rel-induced transformation model of chicken embryonic splenocyte cultures, we show that hematopoietic-specific miR-142 can inhibit the v-rel-induced transformation, and that this inhibition effect is due to the silencing of v-rel expression. The data supports the idea that microRNA-targeting can be used to inhibit viral oncogene-induced oncogenesis. View Full-Text
Keywords: microRNA-targeting; inhibition; v-rel-induced transformation; hematopoietic specific miRNA microRNA-targeting; inhibition; v-rel-induced transformation; hematopoietic specific miRNA
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MDPI and ACS Style

Yao, Y.; Zhang, Y.; Tang, N.; Pedrera, M.; Shen, Z.; Nair, V. Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting. Viruses 2018, 10, 242.

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