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Viruses 2018, 10(4), 212;

Does BCA3 Play a Role in the HIV-1 Replication Cycle?

Department of Biotechnology, University of Chemistry and Technology, Technická 5, 16628 Prague, Czech Republic
Department of Biochemistry and Microbiology, University of Chemistry and Technology, Technická 3, 16628 Prague, Czech Republic
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám 2, 16610 Prague, Czech Republic
Authors to whom correspondence should be addressed.
Received: 20 February 2018 / Revised: 5 April 2018 / Accepted: 18 April 2018 / Published: 20 April 2018
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The cellular role of breast carcinoma-associated protein (BCA3), also known as A-kinase-interacting protein 1 (AKIP-1), is not fully understood. Recently, we reported that full-length, but not C-terminally truncated, BCA3 is incorporated into virions of Mason-Pfizer monkey virus, and that BCA3 enhances HIV-1 protease-induced apoptosis. In the present study, we report that BCA3 is associated with purified and subtilisin-treated HIV particles. Using a combination of immune-based methods and confocal microscopy, we show that the C-terminus of BCA3 is required for packaging into HIV-1 particles. However, we were unable to identify an HIV-1 binding domain for BCA3, and we did not observe any effect of incorporated BCA3 on HIV-1 infectivity. Interestingly, the BCA3 C-terminus was previously identified as a binding site for the catalytic subunit of protein kinase A (PKAc), a cellular protein that is specifically packaged into HIV-1 particles. Based on our analysis of PKAc–BCA3 interactions, we suggest that BCA3 incorporation into HIV-1 particles is mediated by its ability to interact with PKAc. View Full-Text
Keywords: HIV-1; BCA3; AKIP-1; M-PMV; virus incorporation; PKAc HIV-1; BCA3; AKIP-1; M-PMV; virus incorporation; PKAc

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Rumlová, M.; Křížová, I.; Zelenka, J.; Weber, J.; Ruml, T. Does BCA3 Play a Role in the HIV-1 Replication Cycle? Viruses 2018, 10, 212.

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