Does BCA3 Play a Role in the HIV-1 Replication Cycle?
AbstractThe cellular role of breast carcinoma-associated protein (BCA3), also known as A-kinase-interacting protein 1 (AKIP-1), is not fully understood. Recently, we reported that full-length, but not C-terminally truncated, BCA3 is incorporated into virions of Mason-Pfizer monkey virus, and that BCA3 enhances HIV-1 protease-induced apoptosis. In the present study, we report that BCA3 is associated with purified and subtilisin-treated HIV particles. Using a combination of immune-based methods and confocal microscopy, we show that the C-terminus of BCA3 is required for packaging into HIV-1 particles. However, we were unable to identify an HIV-1 binding domain for BCA3, and we did not observe any effect of incorporated BCA3 on HIV-1 infectivity. Interestingly, the BCA3 C-terminus was previously identified as a binding site for the catalytic subunit of protein kinase A (PKAc), a cellular protein that is specifically packaged into HIV-1 particles. Based on our analysis of PKAc–BCA3 interactions, we suggest that BCA3 incorporation into HIV-1 particles is mediated by its ability to interact with PKAc. View Full-Text
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Rumlová, M.; Křížová, I.; Zelenka, J.; Weber, J.; Ruml, T. Does BCA3 Play a Role in the HIV-1 Replication Cycle? Viruses 2018, 10, 212.
Rumlová M, Křížová I, Zelenka J, Weber J, Ruml T. Does BCA3 Play a Role in the HIV-1 Replication Cycle? Viruses. 2018; 10(4):212.Chicago/Turabian Style
Rumlová, Michaela; Křížová, Ivana; Zelenka, Jaroslav; Weber, Jan; Ruml, Tomáš. 2018. "Does BCA3 Play a Role in the HIV-1 Replication Cycle?" Viruses 10, no. 4: 212.
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