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Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

1
School of Plant Protection, Anhui Agricultural University, Hefei 230036, China
2
State Key Laboratory for Plant Disease and Insect Pest, Institute of Plant protection, China Academy of Agricultural Sciences, Beijing 100193, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Viruses 2018, 10(12), 717; https://doi.org/10.3390/v10120717
Received: 29 October 2018 / Revised: 6 December 2018 / Accepted: 13 December 2018 / Published: 15 December 2018
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
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Abstract

The strawberry vein banding virus (SVBV) open reading frame (ORF) VI encodes a P6 protein known as the RNA silencing suppressor. This protein is known to form inclusion like granules of various sizes and accumulate in both the nuclei and the cytoplasm of SVBV-infected plant cells. In this study, we have determined that the P6 protein is the only trans-activator (TAV) encoded by SVBV, and can efficiently trans-activate the translation of downstream gfp mRNA in a bicistron derived from the SVBV. Furthermore, the P6 protein can trans-activate the expression of different bicistrons expressed by different caulimovirus promoters. The P6 protein encoded by SVBV from an infectious clone can also trans-activate the expression of bicistron. Through protein-protein interaction assays, we determined that the P6 protein could interact with the cell translation initiation factor FveIF3g of Fragaria vesca and co-localize with it in the nuclei of Nicotiana benthamiana cells. This interaction reduced the formation of P6 granules in cells and its trans-activation activity on translation. View Full-Text
Keywords: strawberry vein banding virus; P6 protein; trans-activate; bicistron; FveIF3g strawberry vein banding virus; P6 protein; trans-activate; bicistron; FveIF3g
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Li, S.; Hu, Y.; Jiang, L.; Rui, P.; Zhao, Q.; Feng, J.; Zuo, D.; Zhou, X.; Jiang, T. Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g. Viruses 2018, 10, 717.

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