Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay
AbstractWe have developed a generalizable “smart molecular diagnostic” capable of accurate point-of-care (POC) detection of variable nucleic acid targets. Our isothermal assay relies on multiplex execution of four loop-mediated isothermal amplification reactions, with primers that are degenerate and redundant, thereby increasing the breadth of targets while reducing the probability of amplification failure. An easy-to-read visual answer is computed directly by a multi-input Boolean OR logic gate (gate output is true if either one or more gate inputs is true) signal transducer that uses degenerate strand exchange probes to assess any combination of amplicons. We demonstrate our methodology by using the same assay to detect divergent Asian and African lineages of the evolving Zika virus (ZIKV), while maintaining selectivity against non-target viruses. Direct analysis of biological specimens proved possible, with crudely macerated ZIKV-infected Aedes aegypti mosquitoes being identified with 100% specificity and sensitivity. The ease-of-use with minimal instrumentation, broad programmability, and built-in fail-safe reliability make our smart molecular diagnostic attractive for POC use. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 1000 KB)
Share & Cite This Article
Bhadra, S.; Saldaña, M.A.; Han, H.G.; Hughes, G.L.; Ellington, A.D. Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay. Viruses 2018, 10, 714.
Bhadra S, Saldaña MA, Han HG, Hughes GL, Ellington AD. Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay. Viruses. 2018; 10(12):714.Chicago/Turabian Style
Bhadra, Sanchita; Saldaña, Miguel A.; Han, Hannah G.; Hughes, Grant L.; Ellington, Andrew D. 2018. "Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay." Viruses 10, no. 12: 714.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.