Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Cells, Viruses, and Reagents
2.3. Plasmids
2.4. RNA Interference
2.5. Luciferase Reporter Assay and ELISA
2.6. RT-PCR and Quantitative RT-PCR
2.7. Preparation and Enzyme Treatment of EV-A71-Infected RNA
2.8. Plaque Assay
2.9. In Vitro Cleavage Assay
2.10. Statistical Analysis
3. Results
3.1. TLR3 is Involved in Detecting EV-A71 Infection to Induce Type I IFN Antiviral Responses
3.2. Double-Stranded Viral RNA Derived from EV-A71 Replication is Essential for TLR3 Detection
3.3. The TLR3 Signaling Pathway is Required for Triggering Type I IFN Response to EV-A71 Infection
3.4. TLR3 Detects EV-A71 Infection To Induce Type I IFN Response in Primary Myeloid Cells
3.5. EV-A71 Infection Decreases the TLR3 Protein Level
3.6. EV-A71 Protease 2A Targets TLR3 to Inhibit Type I IFN Induction
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chen, K.-R.; Yu, C.-K.; Kung, S.-H.; Chen, S.-H.; Chang, C.-F.; Ho, T.-C.; Lee, Y.-P.; Chang, H.-C.; Huang, L.-Y.; Lo, S.-Y.; et al. Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease. Viruses 2018, 10, 689. https://doi.org/10.3390/v10120689
Chen K-R, Yu C-K, Kung S-H, Chen S-H, Chang C-F, Ho T-C, Lee Y-P, Chang H-C, Huang L-Y, Lo S-Y, et al. Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease. Viruses. 2018; 10(12):689. https://doi.org/10.3390/v10120689
Chicago/Turabian StyleChen, Kuan-Ru, Chun-Keung Yu, Szu-Hao Kung, Shun-Hua Chen, Chuan-Fa Chang, Tzu-Chuan Ho, Yi-Ping Lee, Hung-Chuan Chang, Lan-Yin Huang, Shih-Yen Lo, and et al. 2018. "Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease" Viruses 10, no. 12: 689. https://doi.org/10.3390/v10120689
APA StyleChen, K.-R., Yu, C.-K., Kung, S.-H., Chen, S.-H., Chang, C.-F., Ho, T.-C., Lee, Y.-P., Chang, H.-C., Huang, L.-Y., Lo, S.-Y., Chang, J.-C., & Ling, P. (2018). Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease. Viruses, 10(12), 689. https://doi.org/10.3390/v10120689