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High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus

Mediterranean Agronomic Institute of Bari (CIHEAM-IAMB), Via Ceglie 9, 70010 Valenzano, Italy
UMR 1332 Biologie du Fruit et Pathologie, INRA, Université Bordeaux, CS 20032, 33882 Villenave d’Ornon CEDEX, France
Institute for Sustainable Plant Protection, National Research Council (CNR), Via Università 133, 80055 Portici, Italy
Authors to whom correspondence should be addressed.
Viruses 2018, 10(12), 684;
Received: 24 October 2018 / Revised: 28 November 2018 / Accepted: 29 November 2018 / Published: 3 December 2018
(This article belongs to the Special Issue Plant Virus Ecology and Biodiversity)
In a search for viral infections, double-stranded RNA (dsRNA) were recovered from a diseased cyclamen (Cyclamen persicum Mill.) accession (Cic) and analyzed by high-throughput sequencing (HTS) technology. Analysis of the HTS data showed the presence of Fig mosaic emaravirus (FMV) in this accession. The complete sequences of six FMV-Cic RNA genomic segments were determined from the HTS data and using Sanger sequencing. All FMV-Cic RNA segments are similar in size to those of FMV from fig (FMV-Gr10), with the exception of RNA-6 that is one nucleotide longer. The occurrence of FMV in cyclamen was investigated through a small-scale survey, from which four plants (out of 18 tested) were found RT-PCR positive. To study sequence variations of cyclamen isolates of FMV, RT-PCR products generated through the amplification of the partially RNA-dependent RNA polymerase (RdRp, RNA-1), glycoprotein (GP, RNA-2), and nucleocapsid (NCP, RNA-3) genes were explored. The nucleotide sequence identities for cyclamen isolates ranged between 86% and 99% in RNA-1, 93% and 99% in RNA-2, and 98% and 99% in RNA-3, while lower identity levels were observed with the sequences of fig isolates. Based on the phylogenetic tree obtained with a 304-nt fragment of RNA3, all FMV isolates from cyclamens were assigned to a single cluster close to fig isolates from the Mediterranean. FMV was graft-transmitted to healthy cyclamens eliciting symptoms similar to those observed in the Cic accession, thus suggesting a causal role of FMV in the symptoms that prompted the investigation. This is the first report of FMV in a non-fig host, Cyclamen persicum, a finding that may help in the control of the mosaic and mosaic-like diseases of fig and cyclamen, respectively. View Full-Text
Keywords: high-throughput sequencing; cyclamen; Fig mosaic emaravirus high-throughput sequencing; cyclamen; Fig mosaic emaravirus
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MDPI and ACS Style

Elbeaino, T.; Marais, A.; Faure, C.; Trioano, E.; Candresse, T.; Parrella, G. High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus. Viruses 2018, 10, 684.

AMA Style

Elbeaino T, Marais A, Faure C, Trioano E, Candresse T, Parrella G. High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus. Viruses. 2018; 10(12):684.

Chicago/Turabian Style

Elbeaino, Toufic; Marais, Armelle; Faure, Chantal; Trioano, Elisa; Candresse, Thierry; Parrella, Giuseppe. 2018. "High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus" Viruses 10, no. 12: 684.

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