High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Source Material
2.2. Extraction of Double-Stranded RNAs, High-Throughput Sequencing, and Sequence Bioinformatics Analysis
2.3. Extraction of Total Nucleic Acids and PCR Detection of Cyclamen-Infecting Viruses and Phytoplasmas
2.4. Sequence Analyses
2.5. Grafting and Mechanical Transmission of FMV
3. Results and Discussion
3.1. HTS Data Analyses and Identification of FMV
3.2. RT-PCR Detection and Genetic Variability of FMV from Cyclamen Plants
3.3. Phylogenetic Affinities of Cyclamen FMV Isolates
3.4. Etiology of FMV with Mosaic Disease on Cyclamen
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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FMV-Cic | 5′NCR % | CR % | 3′NCR % | Isolate (Accession Number) | |
---|---|---|---|---|---|
nt | nt | aa | nt | Order of Isolates: JS1, SB1, Gr10, CAN01 | |
RNA-1 | 84–100 | 88–97 | 93–96 | 87–99 | (AB697826, AB697827, AM941711, HQ703343) |
RNA-2 | 98–100 | 91–97 | 95–98 | 95–99 | (AB697828, AB697829, FM864225, HQ703344) |
RNA-3 | 93–99 | 96–98 | 98–99 | 90–96 | (AB697843, AB697785, FM991954, HQ703345) |
RNA-4 | 91–100 | 95–98 | 98–99 | 89–98 | (AB697857, AB697865, FM992851, HQ703346) |
RNA-5 | 84–100 | 84–97 | 82–96 | 84–95 | (AB697871, AB697879, HE803826, n.a) |
RNA-6 | 91–100 | 92–98 | 88–99 | 87–95 | (AB697885, AB697893, HE803827, n.a) |
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Elbeaino, T.; Marais, A.; Faure, C.; Trioano, E.; Candresse, T.; Parrella, G. High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus. Viruses 2018, 10, 684. https://doi.org/10.3390/v10120684
Elbeaino T, Marais A, Faure C, Trioano E, Candresse T, Parrella G. High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus. Viruses. 2018; 10(12):684. https://doi.org/10.3390/v10120684
Chicago/Turabian StyleElbeaino, Toufic, Armelle Marais, Chantal Faure, Elisa Trioano, Thierry Candresse, and Giuseppe Parrella. 2018. "High-Throughput Sequencing Reveals Cyclamen persicum Mill. as a Natural Host for Fig Mosaic Virus" Viruses 10, no. 12: 684. https://doi.org/10.3390/v10120684