Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response

Martins-da-Silva, Andrea; Telleria, Erich Loza; Batista, Michel; Marchini, Fabricio Klerynton; Traub-Csekö, Yara Maria; Tempone, Antonio Jorge

doi:10.3390/v10010043

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Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response

Andrea Martins-da-Silva

Erich Loza Telleria

Michel Batista

^2,3,

Fabricio Klerynton Marchini

^2,3,

Yara Maria Traub-Csekö

^1,* and

Antonio Jorge Tempone

^1,*

Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil

Laboratório de Genômica Funcional, Instituto Carlos Chagas—Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Curitiba 81350-010, PR, Brazil

Plataforma Espectrometria de Massas—RPT02H, Instituto Carlos Chagas—Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Curitiba 81350-010, PR, Brazil

Authors to whom correspondence should be addressed.

Viruses 2018, 10(1), 43; https://doi.org/10.3390/v10010043

Received: 11 October 2017 / Revised: 4 December 2017 / Accepted: 5 December 2017 / Published: 18 January 2018

(This article belongs to the Special Issue Antiviral Defense in Invertebrates)

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Abstract

Hematophagous insects transmit infectious diseases. Sand flies are vectors of leishmaniasis, but can also transmit viruses. We have been studying immune responses of Lutzomyia longipalpis, the main vector of visceral leishmaniasis in the Americas. We identified a non-specific antiviral response in L. longipalpis LL5 embryonic cells when treated with non-specific double-stranded RNAs (dsRNAs). This response is reminiscent of interferon response in mammals. We are investigating putative effectors for this antiviral response. Secreted molecules have been implicated in immune responses, including interferon-related responses. We conducted a mass spectrometry analysis of conditioned medium from LL5 cells 24 and 48 h after dsRNA or mock treatment. We identified 304 proteins. At 24 h, 19 proteins had an abundance equal or greater than 2-fold change, while the levels of 17 proteins were reduced when compared to control cells. At the 48 h time point, these numbers were 33 and 71, respectively. The two most abundant secreted peptides at 24 h in the dsRNA-transfected group were phospholipid scramblase, an interferon-inducible protein that mediates antiviral activity, and forskolin-binding protein (FKBP), a member of the immunophilin family, which mediates the effect of immunosuppressive drugs. The transcription profile of most candidates did not follow the pattern of secreted protein abundance. View Full-Text

Keywords: Lutzomyia longipalpis; embryonic LL5 cells; antiviral response; interferon-like response; poly (I:C); exoproteome

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MDPI and ACS Style

Martins-da-Silva, A.; Telleria, E.L.; Batista, M.; Marchini, F.K.; Traub-Csekö, Y.M.; Tempone, A.J. Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response. Viruses 2018, 10, 43. https://doi.org/10.3390/v10010043

AMA Style

Martins-da-Silva A, Telleria EL, Batista M, Marchini FK, Traub-Csekö YM, Tempone AJ. Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response. Viruses. 2018; 10(1):43. https://doi.org/10.3390/v10010043

Chicago/Turabian Style

Martins-da-Silva, Andrea, Erich L. Telleria, Michel Batista, Fabricio K. Marchini, Yara M. Traub-Csekö, and Antonio J. Tempone. 2018. "Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response" Viruses 10, no. 1: 43. https://doi.org/10.3390/v10010043

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Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response

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