Next Article in Journal
Current and Novel Inhibitors of HIV Protease
Previous Article in Journal
Henipaviruses Employ a Multifaceted Approach to Evade the Antiviral Interferon Response
Previous Article in Special Issue
All Known Human Rhinovirus Species Are Present in Sputum Specimens of Military Recruits During Respiratory Infection
Open AccessArticle

A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain

Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children’s Medical Research Institute, Children’s Health Service District, Queensland, Australia
Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women’s Hospital Campus, Queensland, Australia
Author to whom correspondence should be addressed.
Viruses 2009, 1(3), 1204-1208;
Received: 13 October 2009 / Revised: 28 November 2009 / Accepted: 4 December 2009 / Published: 9 December 2009
(This article belongs to the Special Issue Newly Identified Respiratory Viruses)
Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples. View Full-Text
Keywords: influenza; H1N1; real-time; PCR; detection influenza; H1N1; real-time; PCR; detection
MDPI and ACS Style

Rockett, R.J.; Bialasiewicz, S.; Whiley, D.M.; Bletchly, C.; Faux, C.E.; Lambert, S.B.; Nimmo, G.R.; Nissen, M.D.; Sloots, T.P. A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain. Viruses 2009, 1, 1204-1208.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Search more from Scilit
Back to TopTop