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Open AccessArticle

Early Detection and Identification of the Main Fungal Pathogens for Resistance Evaluation of New Genotypes of Forest Trees

1
Forest Biotechnology Group, Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 6 Prospect Nauki, Pushchino, Moscow 142290, Russia
2
Laboratory for Genetics and Biotechnology, Forest Research Institute, National Academy of Sciences of Belarus, 71 Proletarskaya Str., Gomel 246001, Belarus
3
Department of Forest Genetics and Forest Tree Breeding, George-August University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany
4
Laboratory of Population Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina Str. 3, Moscow 119991, Russia
5
Laboratory of Forest Genomics, Genome Research and Education Center, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Akademgorodok, 50a/2, Krasnoyarsk 660036, Russia
6
Department of Ecosystem Sciences and Management, Texas A&M University, College Station, TX 77843-2138, USA
*
Author to whom correspondence should be addressed.
Forests 2018, 9(12), 732; https://doi.org/10.3390/f9120732
Received: 31 October 2018 / Revised: 19 November 2018 / Accepted: 19 November 2018 / Published: 23 November 2018
The growing importance of forest plantations increases the demand for phytopathogen resistant forest trees. This study describes an effective method for early detection and identification of the main fungal phytopathogens in planting material of silver birch (Betula pendula) and downy birch (B. pubescens), based on the estimation of the size of the internal transcribed spacers (ITS1 and ITS2) in the 18S-5.8S-28S rDNA gene cluster, which are species-specific for most micromycetes. The electrophoretic assay of the ITS1 and ITS2 loci has allowed us to identify predominant phytopathogenic fungal species in downy and silver birch in planta. This new molecular genetic method can be used to screen birch and other forest trees for different fungal pathogens to evaluate disease resistance. This information can be useful in breeding new genotypes of forest trees, including transgenic clones with modified wood composition. View Full-Text
Keywords: Betula; birch; fungal phytopathogens; ITS Betula; birch; fungal phytopathogens; ITS
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Shestibratov, K.A.; Baranov, O.Y.; Subbotina, N.M.; Lebedev, V.G.; Panteleev, S.V.; Krutovsky, K.V.; Padutov, V.E. Early Detection and Identification of the Main Fungal Pathogens for Resistance Evaluation of New Genotypes of Forest Trees. Forests 2018, 9, 732.

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