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Volume 14
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10.3390/f14020215
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Article

Function of Tryptophan 2,3-Dioxygenase in Monochamus alternatus Hope Revealed by RNA Interference

by
Liang-Jing Sheng
1,2,†,
Xiao-Qian Weng
1,2,†,
Ming-Qing Weng
1,2,†,
Ya-Jie Guo
1,3,
Rebeca Carballar-Lejarazú
4,
Fei-Ping Zhang
1,2,* and
Song-Qing Wu
1,2,*
1
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
Laboratory of Forest Symbiology, Asian Research Center for Bioresource and Environmental Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 188-0002, Japan
4
Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA 92697, USA
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Forests 2023, 14(2), 215; https://doi.org/10.3390/f14020215
Submission received: 25 November 2022 / Revised: 18 January 2023 / Accepted: 20 January 2023 / Published: 23 January 2023
(This article belongs to the Section Forest Ecophysiology and Biology)
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Abstract

Monochamus alternatus Hope (Coleoptera: Cerambycidae), an invasive beetle that has caused billions of dollars of economic losses, is a serious pest of Pinus massoniana in many Asian countries. An efficient RNAi system is helpful for functional genomics research on M. alternatus. In this study, a tryptophan 2,3-dioxygenase (TDO) related to the ommochrome synthesis in insects was identified. Using RNAi technology, the M. alternatus TDO gene was silenced by injecting dsRNA into pupae, and individuals were analyzed by phenotype and expression of the TDO gene by RT-qPCR. The results show that TDO is expressed in different developmental stages of M. alternatus, having its peak expression during the prepupal stage. White-eye phenotypes were observed in the pupal and adult stages after dsRNA injection, and a significant 81% decrease in TDO mRNA levels 48 h after injection was determined by RT-qPCR. This gene can be used as a genetic marker and is an important discovery for future genetic engineering tools to control M. alternatus populations.
Keywords: pine wilt disease; dsRNA; TDO gene; white gene; eye pigmentation pine wilt disease; dsRNA; TDO gene; white gene; eye pigmentation

Share and Cite

MDPI and ACS Style

Sheng, L.-J.; Weng, X.-Q.; Weng, M.-Q.; Guo, Y.-J.; Carballar-Lejarazú, R.; Zhang, F.-P.; Wu, S.-Q. Function of Tryptophan 2,3-Dioxygenase in Monochamus alternatus Hope Revealed by RNA Interference. Forests 2023, 14, 215. https://doi.org/10.3390/f14020215

AMA Style

Sheng L-J, Weng X-Q, Weng M-Q, Guo Y-J, Carballar-Lejarazú R, Zhang F-P, Wu S-Q. Function of Tryptophan 2,3-Dioxygenase in Monochamus alternatus Hope Revealed by RNA Interference. Forests. 2023; 14(2):215. https://doi.org/10.3390/f14020215

Chicago/Turabian Style

Sheng, Liang-Jing, Xiao-Qian Weng, Ming-Qing Weng, Ya-Jie Guo, Rebeca Carballar-Lejarazú, Fei-Ping Zhang, and Song-Qing Wu. 2023. "Function of Tryptophan 2,3-Dioxygenase in Monochamus alternatus Hope Revealed by RNA Interference" Forests 14, no. 2: 215. https://doi.org/10.3390/f14020215

APA Style

Sheng, L.-J., Weng, X.-Q., Weng, M.-Q., Guo, Y.-J., Carballar-Lejarazú, R., Zhang, F.-P., & Wu, S.-Q. (2023). Function of Tryptophan 2,3-Dioxygenase in Monochamus alternatus Hope Revealed by RNA Interference. Forests, 14(2), 215. https://doi.org/10.3390/f14020215

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