Next Article in Journal
Molecular Research on Stress Responses in Quercus spp.: From Classical Biochemistry to Systems Biology through Omics Analysis
Next Article in Special Issue
Experimental Examination of Vegetative Propagation Methods of Nothofagus antarctica (G. Forst.) Oerst. for Restoration of Fire-Damaged Forest in Torres del Paine National Park, Chile
Previous Article in Journal
Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
Previous Article in Special Issue
Hybridization Assays in Strawberry Tree toward the Identification of Plants Displaying Increased Drought Tolerance

Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds

Center for Functional Ecology, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal
Department of Forestry Science, NEIKER-BRTA, 01080 Arkaute, Spain
Department of Technology of Organic Substances and Polymer Materials, Tomsk Polytechnic University, 634003 Tomsk, Russia
Center for Nanosciences and Nanotechnology, National Autonomous University of Mexico, 82763 Ensenada, Mexico
Author to whom correspondence should be addressed.
Academic Editor: Gaetano Distefano
Forests 2021, 12(3), 363;
Received: 11 March 2021 / Accepted: 16 March 2021 / Published: 19 March 2021
(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)
Organogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still the main restriction in the clonal propagation of many woody species, especially in conifers. They undergo a “phase change” that leads to significant loss of vegetative propagation capacity, reducing the aptitude of tissues and organs to be regenerated in vitro beyond this point. In line with this, the in vitro regeneration of mature conifer trees has been a long-cherished goal in many laboratories worldwide. Based on previous works in Pinus species regeneration from adult trees, we now present data about the culture of apical shoot buds in an attempt to induce organogenesis and somatic embryogenesis to clone mature trees of Aleppo pine (Pinus halepensis). Reinvigorated axillary shoots were submitted to conditions usually applied to induce somatic embryogenesis through the manipulation of culture media, including the use of auxins such as 2,4-Dichlorophenoxyacetic acid and 1-Naphthaleneacetic acid, cytokinins (6-benzyladenine and kinetin), and phytosulfokine (50, 100, and 200 nM). Although somatic embryos could not be obtained, an embryogenic-like tissue was produced, followed by the emergence of actively proliferating non-embryogenic calli. Variations in the consistence, texture, and color of non-embryogenic calli were observed; especially those arising in the media containing phytosulfokine. Reinvigorated shoots, induced by 22 or 44 µM 6-benzyladenine, were obtained through organogenesis and acclimatized, and phenotypically normal plants were obtained. View Full-Text
Keywords: Aleppo pine; conifers; phytosulfokine; plant growth regulators; rooting Aleppo pine; conifers; phytosulfokine; plant growth regulators; rooting
Show Figures

Figure 1

MDPI and ACS Style

Pereira, C.; Montalbán, I.A.; Pedrosa, A.; Tavares, J.; Pestryakov, A.; Bogdanchikova, N.; Canhoto, J.; Moncaleán, P. Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds. Forests 2021, 12, 363.

AMA Style

Pereira C, Montalbán IA, Pedrosa A, Tavares J, Pestryakov A, Bogdanchikova N, Canhoto J, Moncaleán P. Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds. Forests. 2021; 12(3):363.

Chicago/Turabian Style

Pereira, Cátia, Itziar A. Montalbán, Ana Pedrosa, Jéssica Tavares, Alexey Pestryakov, Nina Bogdanchikova, Jorge Canhoto, and Paloma Moncaleán. 2021. "Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds" Forests 12, no. 3: 363.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop