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Open AccessArticle

Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings

1
Department of Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain
2
Forest Science and Technology Centre of Catalonia, Crta. Sant Llorenç de Morunys km 2, 25280 Solsona, Spain
3
Joint Research Unit CTFC-AGROTECNIO, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain
*
Author to whom correspondence should be addressed.
Forests 2020, 11(8), 792; https://doi.org/10.3390/f11080792
Received: 22 June 2020 / Revised: 16 July 2020 / Accepted: 20 July 2020 / Published: 22 July 2020
(This article belongs to the Special Issue Non-wood Forest Products)
Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process and the root development of Portuguese oak (Quercus faginea Lam.) seedlings destined for truffle plantations has not been determined. The main aim of this study was to shed light on the effect of bacterial inoculation on fungal root tip colonization and seedling root traits. Material and methods: We performed a co-inoculation trial using three bacteria naturally present in the T. melanosporum niche (i.e., Pseudomonas fluorescens, Pseudomonas putida, and Bacillus amyloliquefaciens) and two different bacterial inoculation times (one month and nine months after fungal inoculation) under glasshouse conditions. Results: Only P. fluorescens had a significant mycorrhizal promoter effect, increasing the truffle inoculation rates of root tips by more than 10% compared with seedlings that received non-bacterial inoculation treatments. Simultaneously, the co-inoculation of P. fluorescens with T. melanosporum improved seedling root growth parameters compared with those of seedlings that received non-bacterial inoculation treatments. The different bacterial inoculation times and applications of uninoculated bacterial growth media did not affect the root traits analyzed or the root mycorrhization rates. Conclusions: These results suggest that P. fluorescens bacteria have a potential commercial application as a treatment for truffle-inoculated seedlings to improve both seedling quality and mycorrhizal colonization under nursery conditions. View Full-Text
Keywords: black truffle; truffle colonization; edible fungi cultivation; truffle orchard; truffle trees; hypogeous ectomycorrhizal fungi black truffle; truffle colonization; edible fungi cultivation; truffle orchard; truffle trees; hypogeous ectomycorrhizal fungi
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MDPI and ACS Style

Piñuela, Y.; G. Alday, J.; Oliach, D.; Bolaño, F.; Colinas, C.; Bonet, J.A. Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings. Forests 2020, 11, 792. https://doi.org/10.3390/f11080792

AMA Style

Piñuela Y, G. Alday J, Oliach D, Bolaño F, Colinas C, Bonet JA. Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings. Forests. 2020; 11(8):792. https://doi.org/10.3390/f11080792

Chicago/Turabian Style

Piñuela, Yasmine; G. Alday, Josu; Oliach, Daniel; Bolaño, Francesc; Colinas, Carlos; Bonet, José A. 2020. "Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings" Forests 11, no. 8: 792. https://doi.org/10.3390/f11080792

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