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Open AccessArticle

Poly(ε-caprolactone) Scaffolds Fabricated by Melt Electrospinning for Bone Tissue Engineering

by Sascha Zaiss 1,2,†, Toby D. Brown 1,†, Johannes C. Reichert 1,3 and Arne Berner 1,2,*
Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland 4001, Australia
Department of Trauma Surgery, University Hospital Regensburg, Regensburg 93055, Germany
Department of Orthopedics and Trauma Surgery, Evangelisches Waldkrankenhaus Spandau, Berlin 13589, Germany
Author to whom correspondence should be addressed.
These authors contributed equally to this work. The first two authors should be regarded as joint first authors.
Academic Editor: Michael Nerlich
Materials 2016, 9(4), 232;
Received: 23 January 2016 / Revised: 6 March 2016 / Accepted: 17 March 2016 / Published: 25 March 2016
(This article belongs to the Special Issue Regenerative Materials)
Melt electrospinning is a promising approach to manufacture biocompatible scaffolds for tissue engineering. In this study, melt electrospinning of poly(ε-caprolactone) onto structured, metallic collectors resulted in scaffolds with an average pore size of 250–300 μm and an average fibre diameter of 15 μm. Scaffolds were seeded with ovine osteoblasts in vitro. Cell proliferation and deposition of mineralised extracellular matrix was assessed using PicoGreen® (Thermo Fisher Scientific, Scoresby, Australia) and WAKO® HR II (WAKO, Osaka, Japan) calcium assays. Biocompatibility, cell infiltration and the growth pattern of osteoblasts on scaffolds was investigated using confocal microscopy and scanning electron microscopy. Osteoblasts proliferated on the scaffolds over an entire 40-day culture period, with excellent survival rates and deposited mineralized extracellular matrix. In general, the 3D environment of the structured melt electrospun scaffold was favourable for osteoblast cultures. View Full-Text
Keywords: melt electrospinning; osteoblast; tissue engineering; bone; ovine melt electrospinning; osteoblast; tissue engineering; bone; ovine
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Zaiss, S.; Brown, T.D.; Reichert, J.C.; Berner, A. Poly(ε-caprolactone) Scaffolds Fabricated by Melt Electrospinning for Bone Tissue Engineering. Materials 2016, 9, 232.

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