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Open AccessArticle

Study on Electrochemical Insulin Sensing Utilizing a DNA Aptamer-Immobilized Gold Electrode

Graduate School of Engineering, Soka University, 1-236 Tangi, Hachioji, Tokyo, 192-8577, Japan
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Author to whom correspondence should be addressed.
Academic Editor: Jun-ichi Anzai
Materials 2015, 8(8), 4710-4719; https://doi.org/10.3390/ma8084710
Received: 30 June 2015 / Revised: 14 July 2015 / Accepted: 21 July 2015 / Published: 24 July 2015
We investigated an insulin-sensing method by utilizing an insulin-binding aptamer IGA3, which forms an anti-parallel G-quadruplex with folded single strands. Spectroscopic observation indicates that some anti-parallel G-quadruplex bind hemin and show peroxidase activity. In this study, the peroxidase activity of IGA3 with hemin was confirmed by spectrophotometric measurements, i.e., the activity was three-times higher than hemin itself. IGA3 was then immobilized onto a gold electrode to determine its electrochemical activity. The peroxidase activity of the immobilized IGA3-hemin complex was determined by cyclic voltammetry, and a cathodic peak current of the electrode showed a dependence on the concentration of H2O2. The cathodic peak current of the IGA3-hemin complex decreased by binding it to insulin, and this decrease depended on the concentration of insulin. View Full-Text
Keywords: DNA aptamer; hemin; insulin; glucagon; G-quadruplex; peroxidase activity; gold electrode DNA aptamer; hemin; insulin; glucagon; G-quadruplex; peroxidase activity; gold electrode
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Kubo, I.; Eguchi, T. Study on Electrochemical Insulin Sensing Utilizing a DNA Aptamer-Immobilized Gold Electrode. Materials 2015, 8, 4710-4719.

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