Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Engineering of Chitosan-Coated Collagen Membranes
2.3. Material Characterization
2.3.1. Fourier Transform Infrared (FTIR) Characterization
2.3.2. Scanning Electron Microscopy (SEM) Characterization
2.3.3. X-ray Photoelectron Spectroscopy (XPS) Characterization
2.3.4. Mechanical Properties and Tensile Strength Characterization
2.3.5. Evaluate Potential Degradation of the Membranes
2.4. Chondrocyte Culture on Chitosan-Coated Collagen Membranes
2.4.1. Cell Adhesion as Determined by Hoechst Staining
2.4.2. Investigation of Cell Proliferation by Mean of MTT Assay
2.4.3. Evaluation of the Number of Live Cells after Culture on Chitosan-Coated Collagen Membranes
2.4.4. Interleuk-6 Quantification Following Chondrocyte Culture on Chitosan-Coated Collagen Membranes
3. Statistical Analyses
4. Results and Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Mighri, N.; Mao, J.; Mighri, F.; Ajji, A.; Rouabhia, M. Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion. Materials 2015, 8, 7673-7689. https://doi.org/10.3390/ma8115413
Mighri N, Mao J, Mighri F, Ajji A, Rouabhia M. Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion. Materials. 2015; 8(11):7673-7689. https://doi.org/10.3390/ma8115413
Chicago/Turabian StyleMighri, Nabila, Jifu Mao, Frej Mighri, Abdallah Ajji, and Mahmoud Rouabhia. 2015. "Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion" Materials 8, no. 11: 7673-7689. https://doi.org/10.3390/ma8115413