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Open AccessArticle

Reduction of Adipose Tissue Formation by the Controlled Release of BMP-2 Using a Hydroxyapatite-Coated Collagen Carrier System for Sinus-Augmentation/Extraction-Socket Grafting

1
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul 03722, Korea
2
Department of Applied Life Science, BK21 PLUS Project, College of Dentistry, Yonsei University, Seoul 03722, Korea
3
School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea
4
Department of Orthopaedic Surgery, Ilsan Hospital, Dongguk University, Goyang 10326, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Wen-Hsiang Hsieh
Materials 2015, 8(11), 7634-7649; https://doi.org/10.3390/ma8115411
Received: 11 August 2015 / Revised: 29 October 2015 / Accepted: 4 November 2015 / Published: 11 November 2015
(This article belongs to the Special Issue Selected Papers from ICBEI2015)
The effects of hydroxyapatite (HA)-coating onto collagen carriers for application of recombinant human bone morphogenetic protein 2 (rhBMP-2) on cell differentiation in vitro, and on in vivo healing patterns after sinus-augmentation and alveolar socket-grafting were evaluated. In vitro induction of osteogenic/adipogenic differentiation was compared between the culture media with rhBMP-2 solution and with the released rhBMP-2 from the control collagen and from the HA-coated collagen. Demineralized bovine bone and collagen/HA-coated collagen were grafted with/without rhBMP-2 in sinus-augmentation and tooth-extraction-socket models. Adipogenic induction by rhBMP-2 released from HA-coated collagen was significantly reduced compared to collagen. In the sinus-augmentation model, sites that received rhBMP-2 exhibited large amounts of vascular tissue formation at two weeks and increased adipose tissue formation at eight weeks; this could be significantly reduced by using HA-coated collagen as a carrier for rhBMP-2. In extraction-socket grafting, dimensional reduction of alveolar ridge was significantly decreased at sites received rhBMP-2 compared to control sites, but adipose tissue was increased within the regenerated socket area. In conclusion, HA-coated collagen carrier for Escherichia coli-derived rhBMP-2 (ErhBMP-2) may reduce in vitro induction of adipogenic differentiation and in vivo adipose bone marrow tissue formation in bone tissue engineering by ErhBMP-2. View Full-Text
Keywords: bone morphogenetic protein; collagen; hydroxyapatite; drug delivery system; bone regeneration bone morphogenetic protein; collagen; hydroxyapatite; drug delivery system; bone regeneration
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MDPI and ACS Style

Lee, J.-S.; Kim, T.-W.; Park, S.; Kim, B.-S.; Im, G.-I.; Cho, K.-S.; Kim, C.-S. Reduction of Adipose Tissue Formation by the Controlled Release of BMP-2 Using a Hydroxyapatite-Coated Collagen Carrier System for Sinus-Augmentation/Extraction-Socket Grafting. Materials 2015, 8, 7634-7649.

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