Differences in the Structure and Antimicrobial Activity of Hydrazones Derived from Methyl 4-Phenylpicolinimidate

Abstract

Four novel methyl 4-phenylpicolinoimidate derivatives of hydrazone have been synthesized and evaluated for their antimicrobial activity, including tuberculostatic activity. The compounds obtained are condensates of hydrazonamide or hydrazide with 5-nitro-2-furaldehyde or 5-nitro-2-thiophenecarboxaldehyde. The antimicrobial activity of the tested compounds varied. Compound 3b exhibited significant activity against the tested Gram-positive bacteria (7.8–250 µg/mL). The results of structural tests revealed that the compound is the only one obtained in the form of a Z isomer. Tuberculostatic activity tests showed higher activity of derivatives 3a and 4a containing nitrofuran systems (MICs 3.1–12.5 µg/mL). This research allowed us to identify hydrazone 3b as a starting point for further optimization in the search for antimicrobial drugs. Likewise, compound 4a appears to be a good guiding structure for use in future research on new anti-tuberculosis drugs.

1. Introduction

An increase in the number of infections caused by resistant strains of pathogenic microorganisms has been observed for several decades. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis [1,2,3,4]. Its treatment requires the combined use of several chemotherapeutic agents and lasts up to 24 months [5,6,7]. Currently, in Europe, the total number of infections caused by tuberculosis bacilli remains unchanged, while the number of infections caused by multidrug-resistant strains (MDR-TB) is significantly rising. The treatment of tuberculosis is difficult because the most efficient drugs, such as isoniazid (INH) or rifampicin (RMP), lose their effectiveness against resistant strains. As a consequence, it becomes necessary to extend treatment and use second-line drugs with greater toxicity (e.g., ethionamide, cycloserine) [8,9]. According to the World Health Organization, 5.8 million people fell ill with tuberculosis in 2020. Half a million were cases resistant to RMP (one of the first-line drugs), 48% of which were also MDR-TB. These numbers may change dramatically as a result of increased migration across the continent Unfortunately, in 2021, only one in three patients entered treatment while 1.3 mln patients died [10].

The same is true of other infectious diseases caused by bacteria and fungi. Such infections remain a major health problem due to their resistance to currently used chemotherapeutic agents. Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant strains of Enterococcus (VRE) are particularly dangerous, causing the most lethal hospital infections. A disturbing fact is the growing resistance of Gram-negative bacteria to carbapenems and polymyxins, which are used in therapy as the last resort. The reasons for this phenomenon are attributed to the excessive exposure of the entire community to antibiotics and incorrect treatments of infectious diseases [11,12,13,14,15]. These phenomena reveal an urgent need for new antimicrobial drugs [10].

Many research studies have shown the biological potential of compounds with a pyridine or pyrazine rings, especially their anti-tuberculosis and anti-microbial activity [16,17,18,19,20,21]. Such systems include clinically used chemotherapeutic agents such as INH and pyrazinamide (PZA). Our previous studies showed that some hydrazinecarbodithioic acid esters and amides derived from pyridinamidrazone or pyrazinamidrazone and pyridincarbohydrazide or pyrazincarbohydrazide exhibit significant anti-tuberculosis activity [22,23,24,25]. The most active compounds from this group showed effectiveness against standard and resistant strains of M. tuberculosis with minimal inhibitory concentration (MIC) values in the range of 3.1–12.5 µg/mL (Figure 1). We also observed the same level of activity for benzimidazoles substituted at the C-2 position with a 4-phenylpicolin moiety [26,27,28]. We assumed that the flatness of these molecules may be a prerequisite for their activity [29,30].

In the present study, we have obtained a series of hydrazone derivatives of 4-phenylpicolinohydrazonamide and 4-phenylpicolinohydrazide. The positive influence of the nitrofuryl moiety on antimicrobial activity, known from the literature and clinical practice [31,32], prompted us to use this structural moiety in the designed compounds. The obtained compounds are condensates of 4-phenylpicolinohydrazonamide or 4-phenyl-picolinohydrazide with 5-nitro-2-furaldehyde or 5-nitro-2-thiophenecarboxaldehyde and are two pairs of analogs differing in only one atom (oxygen or sulfur) in a five-membered heterocyclic ring. Because the classical isoster of the N-H group is the oxygen atom [33], we wanted to investigate whether and how the replacement of the NH moiety in hydrazonamides with oxygen in hydrazides would affect biological activity (Figure 2). In view of the wide spectrum of biological activities of hydrazide derivatives, including antimicrobial activity against Gram-positive and -negative bacteria [34], as well as tuberculostatic activity [35], we decided to obtain not only hydrazonamide derivatives but also hydrazide analogs.

2. Materials and Methods

2.1. Chemistry

All materials and solvents were of analytical reagent grade (Sigma-Aldrich-Merck KgaA, Darmstadt, Germany). Thin-layer chromatography was performed on Merck silica gel 60F₂₅₄ plates and visualized with UV light. The results of elemental analyses (%C, H, N) for all of the obtained compounds were in agreement with the calculated values that were within the ±0.4 % range. The ¹H and ¹³C NMR spectra in CDCl₃ or DMSO-d₆ were recorded on Varian Unity Plus (500 MHz) and Varian Gemini (200 MHz) instruments (Varian Medical Systems, Palo Alto, CA, USA). IR Spectra (KBr) were determined as KBr pellets of the solids on a Satellite FT-IR spectrophotometer (Bruker, Madison, WI, USA). Melting points were determined using a Stuart SMP30 apparatus (Stone, Staffordshire, UK) and were retained without any corrections.

2.1.1. Methyl 4-Phenylpicolinimidate

To a solution of 4-phenylpicolinonitrile (1.8 g, 10 mmol) in methanol (30 mL) was added DBU (1,8-diazabicyclo[5.4.0]undec-7-ene, 1 mL, 7 mmol); the mixture was refluxed for 1 h. Then, the solvent was evaporated and the oily residue was treated with cyclohexane and SiO₂ (1 g) and stirred for 5 h. The silica gel was filtered off and the solvent evaporated. The purified oily product crystallized after cooling, giving straw crystals (1.8 g, 85%): m.p. 80–82 °C; IR (KBr): 3298 (υ N-H), 3053, 2947 (υ C-H), 1654 (υ C=N), 1591 (δ N-H), 1440, 1358 (υ C=C), 1081 (υ C-O), 964 (δ C-H), 871, 758, 704 (γ C-H) cm⁻¹; ¹H NMR (200 MHz, DMSO-d₆): δ 3.93 (s, 3H, CH₃), 6.95 (dd, 1H, pyridine, J¹ = 5 Hz, J² = 2 Hz), 6.89–7.25 (m, 5H, PhH), 8.05 (s, 1H, pyridine), 8.36 (dd, 1H, pyridine, J¹ = 5 Hz, J² = 1 Hz), 10.04 (s, 1H, NH) ppm; Anal. Calcd. for C₁₃H₁₂N₂O (212.09): C, 73.56; H, 5.70; N, 13.20; Found: C, 73.25; H, 5.37; N, 13.52. All data are consistent with a description in the literature [36].

2.1.2. 4-Phenylpicolinohydrazonamide (1)

Methyl 4-phenylpicolinimidate (2.1 g, 10 mmol) was dissolved in methanol (10 mL), 98% hydrazine hydrate (3 mL, 95 mmol) was added, and the solution was refluxed for 1 h. Then, the mixture was cooled and water (30 mL) was added. The precipitate was filtered off and recrystallized using benzene, giving the compound 1 as a yellow solid (1.8 g, 85%): m.p. 110–111 °C; IR (KBr): 3444, 3395, 3351, 3310 (υ N-H), 3058, 2923 (υ C-H), 1646, 1597 (υ C=N), 1541 (δ N-H), 1467, 1427 (υ C=C), 883, 752, 696 (γ C-H) cm⁻¹; ¹H NMR (500 MHz, CDCl₃): δ 4.65 (s, 2H, NH2 + D₂O exchangeable), 5.36 (s, 2H, NH₂ + D₂O exchangeable), 7.44–7.55 (m, 4H, Ph), 7.71–7.77 (m, 2H, 1H Ph and 1H pyridine), 8.30 (s, 1H, pyridine), 8.58 (d, 1H, pyridine, J = 5 Hz) ppm; ¹³C NMR (175 MHz, CDCl₃): δ 117.02, 120.06, 128.55 (2C), 129.46 (2C), 129.55, 138.00, 148.34, 148.90, 151.40, 157.85 ppm; Anal. Calcd. for C₁₂H₁₂N₄ (212.11): C, 67.90; H, 5.70; N, 26.40; Found: C, 68.16; H, 5.41; N, 26.12.

2.1.3. 4-Phenylpicolinohydrazide (2)

Methyl 4-phenylpicolinimidate (3.3 g, 17 mmol) was dissolved in methanol (15 mL) and 10% HCl (15 mL) was added while cooling. The solution turned clear at first and then became cloudy. After 0.5 h, the precipitate of methyl ester was filtered off and washed with saturated NaHCO₃ solution and then with water. Then, ethanol (5 mL) was added to the precipitate and 98% hydrazine hydrate (3 mL, 95 mmol). The solution was refluxed for 1 h. The mixture was cooled and water (30 mL) was added. The precipitate of hydrazide was filtered off and recrystallized using ethanol, giving the title compound 2 as yellow solid (2.3 g, 85%): m.p. 106–107 °C; IR (KBr): 3412, 3315, 3267 (υ N-H), 3062 (υ C-H), 1685 (υ C=O), 1598, 1542 (δ N-H), 1510, 1498 (υ C=C), 962 (δ C-H), 764, 705, 690 (γ C-H) cm⁻¹; ¹H NMR (200 MHz, DMSO-d₆): δ 4.62 (d, 2H, NH₂ + D₂O exchangeable, J = 4 Hz), 7.47–7.60 (m, 3H, Ph), 7.83–7.90 (m, 2H, 1H Ph and 1H pyridine), 8.24 (s, 1H, pyridine), 8.66 (d, 1H, pyridine, J = 5 Hz), 9.97 (br s, 1H, NH + D₂O exchangeable) ppm; Anal. Calcd. for C₁₂H₁₁N₃O (213.09): C, 67.59; H, 5.20; N, 19.71; Found: C, 67.70; H, 5.34; N, 19.40.

2.1.4. General Procedure for the Synthesis of Hydrazones (3a,b–4a,b)

Compound 1 (0.21 g, 1 mmol) or compound 2 (0.21 g, 1 mmol) was dissolved in methanol (10 mL) and an appropriate aldehyde was added (1 mmol). The mixture was refluxed for 0.5 h and then left to crystallize. The precipitate was filtered off and recrystallized.

(E)-N′-((5-Nitrofuran-2-yl)methylene)-4-phenylpicolinohydrazonamide (3a)

Starting from 5-nitrofuran-2-carbaldehyde (0.14 g), compound 3a was obtained as an orange solid (0.27 g, 80%): m.p. 207–208 °C (dioxane-water 1:1); IR (KBr): 3390, 3323 (υ N-H), 3163, 2923 (υ C-H), 1613 (υ C=N), 1578 (υ NO₂), 1513, 1461 (υ C=C), 1351 (υ NO₂), 1304, 1181 (υ C-O), 1008 (δ C-H), 810, 756 (γ C-H) cm⁻¹; ¹H NMR (200 MHz, DMSO-d₆): δ 7.41–7.63 (m, 6H, 2H Ph and 1H CH and 1H furan and 2H NH₂ + D₂O exchangeable), 7.81–7.93 (m, 4H, 3H Ph and 1H furan), 8.44 (s, 1H, pyridine), 8.50 (m, 1H, pyridine), 8.75 (d, 1H, pyridine, J = 5 Hz) ppm; ¹³C NMR (175 MHz, DMSO-d₆): δ 114.86, 115.64, 119.31, 123.87, 127.42 (2C), 129.85 (2C), 130.07, 137.31, 142.19, 148.60, 149.95, 150.87, 152.24, 154.14, 158.87 ppm; Anal. Calcd. for C₁₇H₁₃N₅O₃ (335.10): C, 60.89; H, 3.91; N, 20.89; Found: C, 60.93; H, 3.60; N, 20.73.

(Z)-N′-((5-Nitrothiophen-2-yl)methylene)-4-phenylpicolinohydrazonamide (3b)

Starting from 5-nitrothiophene-2-carbaldehyde (0.16 g), compound 3b was obtained as a yellow solid (0.30 g, 85%): m.p. 156–157 °C (dioxane-water 1:1); IR (KBr): 3448, 3303 (υ N-H), 1618 (υ C=N), 1569 (υ NO₂), 1524 (υ N-H), 1489, 1421 (υ C=C), 1328 (υ NO₂), 1012, 984 (δ C-H), 815, 753, 686 (γ C-H) cm⁻¹; ¹H NMR (200 MHz, DMSO-d₆): δ 7.56–7.67 (m, 4H, 3H Ph and 1H thiophene), 7.75 (s, 1H, NH + D₂O exchangeable), 7.83 (s, 1H, NH + D₂O exchangeable), 8.00–8.14 (m, 3H, 2H Ph and 1H CH), 8.17 (d, 1H, thiophene, J = 4 Hz), 8.36 (s, 1H, pyridine), 8.80 (d, 1H, pyridine, J = 5 Hz), 8.90–8.91 (m, 1H, pyridine) ppm; ¹³C NMR (175 MHz, DMSO-d₆): δ 114.89, 115.57, 119.31, 123.85, 127.40 (2C), 129.83 (2C), 130.05, 137.33, 142.23, 148.61, 149.93, 150.88, 152.25, 154.09, 158.83 ppm; Anal. Calcd. for C₁₇H₁₃N₅O₂S (351.08): C, 58.11; H, 3.73; N, 19.93; Found: C, 58.37; H, 3.94; N, 20.17.

(E)-N′-((5-Nitrofuran-2-yl)methylene)-4-phenylpicolinohydrazide (4a)

Starting from 5-nitrofuran-2-carbaldehyde (0.14 g), compound 4a was obtained as an orange solid (0.29 g, 88%): m.p. 285–287 °C (methanol); IR (KBr): 3277 (υ N-H), 3106 (υ C-H), 1689 (υ C=O), 1533 (υ NO₂), 1468 (υ C=C), 1354 (υ NO₂), 1248, 1137 (υ C-O), 966 (δ C-H), 810, 766 (γ C-H) cm⁻¹; ¹H NMR (500 MHz, DMSO-d₆): δ 7.28 (d, 1H, furan, J = 4 Hz), 7.51–7.58 (m, 3H Ph), 7.81 (d, 1H, furan, J = 4 Hz), 7.90 (d, 1H, Ph, J = 7 Hz), 8.03 (d, 1H, furan, J = 4 Hz), 8.38 (s, 1H, pyridine), 8.64 (s, 1H, CH), 8.79 (d, 1H, pyridine, J = 5 Hz, 12.78 (1H, NH with D₂O exchangeable) ppm; ¹³C NMR (175 MHz, DMSO-d₆): δ 115.07, 115.55 (2C), 120.54, 124.99, 127.53 (2C), 129.86 (2C), 130.27, 136.88, 137.43, 149.12, 149.83, 150.40, 152.36, 161.33 ppm; Anal. Calcd. for C₁₇H₁₂N₄O₄ (336.09): C, 60.71; H, 3.60; N, 16.66; Found: C, 60.68; H, 3.85; N, 16.99.

(E)-N′-((5-Nitrothiophen-2-yl)methylene)-4-phenylpicolinohydrazide (4b)

Starting from 5-nitrofuran-2-carbaldehyde (0.16 g), compound 4b was obtained as an orange solid (0.30 g, 85%): m.p. 220–222 °C (methanol); IR (KBr): 3249 (υ N-H), 3108, (υ C-H), 1686 (υ C=O), 1539 (υ NO₂), 1522, 1435 (υ C=C), 1335 (υ NO₂), 1047, 1029 (δ C-H), 761, 644 (γ C-H) cm⁻¹; ¹H NMR (500 MHz, DMSO-d₆): δ 7.54–7.56 (m, 3H Ph), 7.75–7.76 (m, 2H Ph), 8.87–8.90 (m, 1H, thiophene), 8.53 (s, 1H, CH), 8.62–8.65 (m, 2H, 1H pyridine and 1H thiophene), 8.69–8.71 (m, 1H pyridine), 8.94–8.96 (m, 1H, pyridine), 11.78 (1H, NH + D₂O exchangeable) ppm; ¹³C NMR (175 MHz, DMSO-d₆): δ 120.50, 125.00, 127.54 (2C), 129.89 (2C), 130.24, 130.31, 130.98, 136.84, 142.83, 147.14, 149.38, 149.84, 150.38, 151.43, 161.20 ppm; Anal. Calcd. for C₁₇H₁₂N₄O₃S (352.06): C, 57.95; H, 3.43; N, 15.90; Found: C, 57.56; H, 3.33; N, 16.12.

2.2. ADMET

The compounds were analyzed for their pharmacokinetic properties, drug-likeness and absorption. An ADME (Absorption, Distribution, Metabolism, and Excretion) analysis was performed using the SwissADME service (Swiss Institute of Bioinformatics 2021): a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules, and BOILED-Egg, to predict the gastrointestinal absorption and brain penetration of the molecules [37]. The bioavailability radar for each compound was determined. ProTOX II provides predictions of the toxicities of compounds [38].

2.3. Biological Activities

2.3.1. In Vitro Antimicrobial Activity Assay

The antibacterial and antifungal activities of the tested compounds were screened by microdilution broth method using Mueller-Hinton broth and Mueller-Hinton broth with 2% glucose for growth of bacteria and fungi, respectively. The minimal inhibitory concentrations (MICs) of the tested derivatives were evaluated for the panel of the reference microorganisms, including Gram-negative bacteria (Proteus mirabilis ATCC 12453, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 13883, Gram-positive bacteria (Micrococcus luteus ATCC 10240, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Bacillus subtilis ATCC 6633, and Bacillus cereus ATCC 10876) and fungi (Candida parapsilosis ATCC 22019) (LGC Standards, Teddington, Middlesex, UK). The antimicrobial assays were performed in the same manner as in our previous research [39]. Vancomycin, Ciprofloxacin, and fluconazole were used as standard drugs. Each experiment was performed in triplicate. Representative data are presented.

2.3.2. Tuberculostatic Activity Assay

The newly synthesized hydrazones were tested in vitro for their tuberculostatic activity toward the M. tuberculosis standard strain, H₃₇Rv (National Tuberculosis and Lung Diseases Research Institute, Warsaw, Poland) and two native strains isolated from tuberculosis patients (National Tuberculosis and Lung Diseases Research Institute, Warsaw, Poland): Spec. 210, resistant to p-aminosalicylic acid (PAS), INH, ethambutol (ETB); and RMP and Spec. 192, fully sensitive to the administrated tuberculostatic drugs. Investigations were performed by a classical test-tube method described in detail earlier [39]. Each experiment was performed in triplicate. Representative data are presented.

2.4. X-ray Study

Monocrystal X-ray diffraction measurements of all compounds were made using a Bruker SMART APEXII CCD Diffractometer (Bruker AXS Inc., Madison, WI, USA) with CuKα radiation at 100 K. The diffraction data were processed with SAINT ver. 8.34 A, SADABS ver. 2014/4 and XPREP ver. 2014/2 (Bruker AXS Inc., Madison, WI, USA). The structures were determined with the ShelXT 2018/2 solution program (Version 2018/2, 2018, Göttingen, Germany) [40] and refined with ShelXL 2018/3 [41]. For visualization, ShelXle [42] was used. The addition and refinement of hydrogen atoms has been described in the previous work [43].

CCDC 2154198, 2154201, 2154207 and 2154204 contain the supplementary crystallographic data for this paper. The data are provided free of charge by The Cambridge Crystallographic Data Center via www.ccdc.cam.ac.uk/structures (accessed on 23 February 2022).

2.5. DFT Calculations

Quantum calculations were performed with GAMESS-US software [44] using DFT/B3LYP [45,46,47] with the base functions 6–311 G (d,p) to optimize the geometry of the studied compounds, taking into account the solvent effect of water, using the polarizable continuum model.

3. Results and Discussion

3.1. Synthesis

The initial compound 4-phenyl-2-cyanopyridine, refluxed for 1 h with DBU in methanol, gave methyl 4-phenylpicolinimidate with 85% yield (Figure 3). Amidrazone 1 was obtained by refluxing methyliminoester with hydrazine hydrate in methanol for 1 h (yield 85%). Treatment of the iminoester with 10% HCl in methanol yielded a methyl ester which, upon reaction with hydrazine hydrate in ethanol, gave hydrazide 2 (71%). Hydrazonamide 1, when refluxed for 0.5 h in methanol with appropriate aldehydes (2-nitrofuryl, 2-nitrothiophene), reacted to condensates 3a,b (yields 80% and 85% respectively) as well as hydrazide 2 to hydrazones 4a,b (yields 88% and 85%, respectively). All the newly synthesized compounds were characterized by IR, ¹H NMR and ¹³C NMR spectra and elemental analysis. The results from the spectral analysis were in accordance with the assigned structures.

3.2. ADMET Analysis

Bioavailability radars were performed for all studied compounds (Figure 4). The ranges for the plot are: lipophility (LIPO) within the range −0.7 < XlogP3 < +5.0; molecular weight (SIZE) is 150 g/mol < MW < 500 g/mol; polarity (POLAR) is 20 Ų < TPSA < 130 Ų; insolubility (INSOLU) is 0 < logS < 6;—insaturation (INSATU) is 0.25 < fraction Csp3 < 1; and flexibility (FLEX) are 0 < num. rotatable bonds < 9. For drug-like properties, the compounds were found to have a good bioavailability score (0.55). All compounds complied with the rules of Lipinski [48], Ghose [49], Egan [50], Veberm [51] and Muegge [52]. This means they are good drug candidates. In the BOILED-Egg diagram (Figure 5), compounds 3a and 4a showed absorption via the gastrointestinal tract, which would make them effective drugs. They are not substrates of P-gp, which means they are good candidates against multidrug resistant cancer cells. The analysis showed that all tested compounds could be tested, while in terms of absorption in the gastrointestinal tract, two of them were very interesting (3a and 4a).

Service ProTox II classified 4a at toxicity class 4 (predicted LD₅₀: 1500 mg/kg, i.e., harmful if swallowed (300 < LD₅₀ ≤ 2000)), 4b at toxicity class 5 (predicted LD₅₀: 3506 mg/kg, i.e., may be harmful if swallowed (2000 < LD₅₀ ≤ 5000)), 3a at toxicity class 4 (predicted LD₅₀: 750 mg/kg) and 3b at toxicity class 3 (predicted LD₅₀: 200 mg/kg, i.e., toxic if swallowed (50 < LD₅₀ ≤ 300)) [38].

3.3. Antimicrobial Activity

All of the obtained compounds were evaluated for their in vitro antimicrobial activity. The first screening of antimicrobial activity was performed on representatives of Gram-positive bacteria, i.e., Staphylococcus epidermis, S. aureus, Micrococcus luteus, Bacillus subtilis, B. cereus; Gram-negative bacteria, i.e., Escherichia coli and Klebsiella pneumonia; and fungi, i.e., Candida parapsilosis. Ciprofloxacin, vancomycin, and fluconazole were used as reference drugs (

Table 1. In vitro antimicrobial activity of compounds ^1,2.

Compound	3a	3b	4a	4b	CIP 2	VAN 2	FCZ 2
Microorganism	MIC 1 [µg/mL]
	Gram-positive bacteria
S. aureus ATCC 25923	1000	7.8	1000	1000	0.49	0.98	-
S. epidermidis ATCC 12228	0.49	7.8	3.9	0.98	0.49	0.98	-
M. luteus ATCC 10240	500	250	1000	62.5	0.98	0.12	-
B. subtilis ATCC 6633	0.49	31.3	125	31.3	0.03	0.24	-
B. cereus ATCC 10876	250	125	125	1000	0.12	0.98	-
	Gram-negative bacteria
E. coli ATCC 25922	1000	1000	>1000	>1000	0.004	-	-
K. pneumoniae ATCC 13883	1000	>1000	>1000	>1000	0.12	-	-
P. mirabilis ATCC 12453	>1000	1000	>1000	>1000	0.03	-	-
	Yeast
C. parapsilosis ATCC 22019	250	250	250	250	-	-	1.95

¹ Each experiment was performed in triplicate. Representative data are presented. ² CIP ciprofloxacin, VAN vancomycin, FCZ fluconazole.

).

The results given in Table 1 indicate that the inhibitions by compounds were lower than those of standard drugs. The most promising compound turned out to be 3b, which demonstrated very strong anti-staphylococcal activity (MIC < 10 µg/mL), good activity against spore-forming bacilli (MIC in the range 31.3–125 µg/mL), and moderate anti-micrococci bioactivity. In general, compounds 3b and 4b with sulfur-containing moieties showed higher activity against the tested Gram-positive bacteria in comparison to compounds 3a and 4a with 5-nitro-furan-2-yl moiety (except with S. aureus, against which they showed mild activity). The possible reason for the antibacterial action of these compounds may be that they bind to the membranes of microorganisms through hydrogen bonding with sulfur, increasing the time to complete cell division; thus, the generation time of the bacteria was prolonged. Moreover, 3a and 3b presented very mild bioactivity against Gram-negative bacteria, whereas 4a and 4b did not demonstrate bioactivity according to accepted definitions [53]. Thus, the antibacterial efficiency of the tested derivatives decreased in the following order: 3b > 3a > 4b > 4a. Moderate bioactivity was found against the representative yeast strain, irrespective of the kind of derivatives. The differences in biological activity of compounds to Gram-positive and -negative bacteria could be explained by the differences in their cell wall structure, and thus, in permeability. Peptydoglycan is major component (90%) of the Gram-positive cell wall. The presence in Gram-negative bacteria of an outer lipid biliayer containing lipopolysaccharide, porins, adhesions creates an additional barrier which must be overcome.

3.4. Tuberculostatic Activity

The newly obtained hydrazones were also tested for tuberculostatic activity against three strains of M. tuberculosis, i.e., the standard strain H37Rv and two native strains from patients, namely, Spec 210, which is resistant to clinically used anti-tuberculosis drugs (PAS, INH, ETB, RMP) and Spec 192, which is completely sensitive. INH and PZA were used as reference drugs. The tested derivatives showed varied tuberculostatic activity with MIC values in the range of 3.1–25 µg/mL (

Table 2. Tuberculostatic activity of tested compounds ^1,2,3.

Compd.	MIC 1 [µg/mL]
	H37Rv 2	Spec. 192	Spec. 210
3a	6.2	6.2	6.2
3b	25	25	25
4a	3.1	12.5	3.1
4b	25	25	25
INH 3	12.5	12.5	25
PZA 3	25	25	>400

The values obtained for the most potent compounds are marked in bold. ¹ Minimal inhibiting concentrations were determined by a classical test-tube method of successive dilution. Each experiment was performed in triplicate. Representative data are presented. ² M. tuberculosis H₃₇Rv, Spec. 192, Spec. 210. ³ INH isoniazid; PZA pyrazinamide.

). However, some of them showed activity that was better than that of reference drug pyrazinamide, with activity against the standard H37Rv strain and susceptible strain Spec. 192 at a MIC level of 25 µg/mL, and against the resistance strain Spec. 210 at over 400 µg/mL, but also INH (MICs of 12.5 µg/mL and 25 µg/mL, respectively). Compound 3a and 4a with 5-nitro-furan-2-yl moieties showed good activity, with MIC values in a range of 3.1–12.5 µg/mL. Compound 3a, i.e., an amidrazone derivative with a 5-nitro-furan-2-yl moiety, showed good activity with an MIC value of 6.2 µg/mL. Its activity was, respectively, four times higher against the standard and resistant strains than its sulfur-containing analog 3b and two times higher against the sensitive strain compared to reference INH. This compound was four times more potent against resistant Spec. 210 than INH. Compound 4a, a hydrazide derivative, showed even better activity with an MIC value of 3.1 µg/mL against both sensitive and resistant strains; it was eight times more active than its sulfur analog 4b and four times more active than reference INH. It is noteworthy that for most compounds, the MIC values against the resistant strain were at the same level as those against the standard strain. One consequence of acquiring resistance is the necessity to use increased doses of chemotherapeutic agents. In our study, the resistant mycobacteria showed exactly the same sensitivity to the compounds tested by us as the non-resistant mycobacteria. This may mean that the tested compounds bypassed the molecular targets which are characteristic of classic anti-tuberculosis chemotherapeutic agents against which resistance has been demonstrated with strain Spec. 210 [54,55].

3.5. X-ray Study

The significant differences in the activities of the studied compounds prompted us to look closely at their spatial structure by X-ray diffraction (Figure 6). Appropriate crystals were obtained by slow evaporation of a DMF-methanol mixture. The basic crystallographic data are presented in

Table 3. Crystal data, data collection and refinement details.

	3a	3b	4a	4b
Chemical formula	C17H13N5O3	C17H13N5O2S	C17H12N4O4	2(C17H12N4O3S)·H2O
Mr	335.32	351.38	336.31	722.75
Crystal system, space group	Monoclinic, P21	Triclinic, P-1	Monoclinic, P21/c	Monoclinic, C2/c
a, b, c (Å)	7.9576 (1), 6.8200 (1), 14.7329 (3)	9.3981 (2), 16.2901 (4), 21.5857 (6)	9.8950 (4), 16.6918 (7), 10.2708 (5)	27.8383 (15), 6.6147 (4), 18.6149 (10)
α, β, γ (°)	90, 101.1060 (4), 90	78.623 (1), 81.641 (1), 75.018 (1)	90, 114.976 (1), 90	90, 112.197 (1), 90
V (Å3)	784.59 (2)	3114.04 (13)	1537.74 (12)	3173.8 (3)
Z	2	8	4	4
Crystal size (mm)	0.34 × 0.18 × 0.17	0.57 × 0.24 × 0.10	0.23 × 0.18 × 0.16	0.45 × 0.42 × 0.32
No. of measured, independent and observed [I > 2σ(I)] reflections	8763, 2625, 2622	60,640, 12,299, 11,734	16,684, 3038, 2922	16,666, 3125, 3114
Rint	0.016	0.025	0.021	0.025
(sin θ/λ)max (Å−1)	0.618	0.619	0.618	0.618
R[F2 > 2σ(F2)], wR(F2), S	0.023, 0.063, 1.06	0.052, 0.128, 1.10	0.032, 0.084, 1.04	0.031, 0.083, 1.07
No. of parameters	233	925	229	234
No. of restraints	1	0	0	0
Δmax, Δmin (e Å−3)	0.19, −0.12	2.56, −0.85	0.29, −0.24	0.42, −0.28
Absolute structure	Flack (1983)	–	–	–
Absolute structure parameter	0.14 (3)	–	–	–

, while Figure 7, Figure 8, Figure 9 and Figure 10 show details of their crystal structures and packing. Powder diffraction diagrams confirmed that the crystals chosen for the structure analysis were representative of the entire powder sample (Figure S1). Crystals of compound 3b revealed some twinning, which was difficult to resolve during data processing, and consequently, the data were reduced, as for a single crystal. Probably because of this unresolved twinning, the residual electron density was much higher for this structure (Table 3). Compounds 3a and 3b are hydrazonamide derivatives. In the case of compound 3a, there was only one molecule in the asymmetric unit, as opposed to compound 3b, in which there were four molecules. Moreover, compound 3b was the only one with the Z conformation. In the case of hydrazide derivatives 4, there was one molecule in an asymmetric unit. Additionally, a water molecule was built into the structure of compound 4b.

In 3a, the molecules form chains by means of N-H · N intermolecular hydrogen bonds (C2,2 (8) type, Figure 7,

Table 4. Hydrogen-bond geometry (Å, °) for 3a.

D—H⋯A	D—H	H⋯A	D⋯A	D—H⋯A
N4—H4A⋯N2 i	0.86 (3)	2.57 (2)	3.2842 (19)	141 (2)
N4—H4B⋯N42 ii	0.93 (3)	2.24 (2)	3.0384 (18)	143 (2)

Symmetry codes: (ⁱ) −x, y − 1/2, −z; (ⁱⁱ) −x, y + 1/2, −z.

), resulting in a layered arrangement of the molecules in the crystals (Figure 7).

In structure 3b, three of the four independent molecules are stabilized by intermolecular hydrogen N-H⋯N bonds (R2,2 (10)) and N-H⋯O ones (Figure 8,

Table 5. Hydrogen bond geometry (Å, °) in 3b.

D—H⋯A	D—H	H⋯A	D⋯A	D—H⋯A
N4B—H4BA⋯N2A	0.86 (3)	2.25 (3)	3.041 (3)	153 (3)
N4D—H4DA⋯O33B	0.87 (4)	2.49 (4)	3.125 (3)	130 (3)
N4A—H4AB⋯N2B	0.86 (3)	2.33 (3)	3.107 (3)	150 (3)

). The fourth molecule (denoted as C) serves as a space filler, as seen from higher displacement parameters of its atoms and standard uncertainties of respective bonds lengths and angles (Tables S1 and S2).

In structure 4a, chains of C1,1 (9) hydrogen bonds are formed. In addition, the flat build of the molecules is stabilized by an intramolecular hydrogen bond of type N3-H⋯N42 (Figure 9,

Table 6. Hydrogen bond geometry (Å, °) in 4a.

D—H⋯A	D—H	H⋯A	D⋯A	D—H⋯A
N3—H3⋯O33 i	0.862 (17)	2.278 (17)	3.1072 (13)	161.6 (17)

Symmetry codes: (ⁱ) x − 1, −y + 3/2, z − 1/2.

). The packing of the molecules resembles that most often found in aromatic (planar) systems.

In structure 4b, rings of hydrogen bound molecules are formed [R3,4 (12)] and stabilized by water molecules, which, in turn, form chains running in direction [010] (

Table 7. Hydrogen-bond geometry (Å, °) in 4b.

D—H⋯A	D—H	H⋯A	D⋯A	D—H⋯A
O1W—H1W⋯O4 i	0.81 (2)	2.14 (2)	2.9372 (13)	169 (2)
N3—H3⋯O1W	0.88	2.39	3.1280 (16)	142

Symmetry codes: (ⁱ) x, y − 1, z.

, Figure 10). In addition, the flatness of the molecules is stabilized by N3-H⋯N42 hydrogen bonds. As a result, a layered structure stabilized by hydrogen bonds with water molecules is formed. In general, the packing resembles, to some degree, that observed in 4a (Figure 10), despite the presence of water in 4b and the different symmetries (space groups) in the two structures 4 (Table 3).

The molecular parameters of the described structures (Tables S1 and S2) are in good agreement with the values found for similar compounds (Scheme S1) in the crystal state (Cambridge Structural Database [56]). They confirm the conjugation system comprising C1-N2-N3-C4-C41(2-pyridine) chains, resulting in the shortening of formally singular N-N bonds to 1.37–1.40 Å (the longest values of about 1.50 Å were observed for singular N-N bonds—see Scheme S1) and the lengthening of formally double bonds (C1=N2 in 3 to 1.28–1.29 Å and N3=C4 in 4 to 1.31 Å). The coplanarity of the central chain fragment with the pyridine ring is secured both by conjugations, as evidenced by the shortening of C4-C41 bonds to about 1.50 Å (from 1.53 for pure aliphatic C4 substituents) and by intramolecular hydrogen bonds (N4-H⋯N42 in 3a,b and N3-H⋯N42 in 4a,b).

The superposition of all the molecules studied here shows general similarities in the conformations of their main chains (C1-N2-N3-C4-C41) (Figure 11). The different positions of the phenyl rings in structures 3 and 4 results from different orientations of the pyridine rings, which, in turn, is the result of different possible intramolecular hydrogen bonds, being N3-H⋯N(pyridine) in 3a,b or N4-H⋯N(pyridine) in 4a,b. Meanwhile, a wide range of phenyl ring rotations (Figure 9) reflects the significant freedom of their movements. On the other hand, the unique position of the nitrothiophene substituent in 3b (Figure 8) results from its Z configuration in the C1=N2 bond, as compared to all other studied structures, which comprised E isomers (Figure 6). The consequence of the Z configuration in 3b is the coiled shape of the molecules (best seen in Figure 8) instead of the elongated form observed for E isomers (Figure 6). We believe that flat molecules are easy to pack and therefore, in most cases, have one independent molecule in an asymmetric unit, while irregularly shaped (or even flat) molecules (like 3b, in contrast to 3a and 4a,b) often need to form clusters of several molecules to pack efficiently. In the case of 3b, there are four independent molecules in the crystal structure (Figure 8).

The most important difference between the studied compounds is the substituent at the C4 atom, being either an amine group (in 3a,b) or an oxygen atom (in 4a,b). The two substituents play the opposite role in the formation of strong hydrogen bonds (as a donor in 3 and an acceptor in 4). This means that the two types of compounds should not interact with the same receptor (or at least not in the same way), as suggested by their similar tuberculostatic activity (Table 2). Still, at a glance, it was surprising that despite this difference, the central parts of both hydrazonamides (3a,b) and hydrazides (4a,b) stretched from C1 to the pyridine ring were approximately planar and had very similar shapes (Figure 11). The planarity is due to the conjugations (Table S1) aided by intramolecular hydrogen bonds N4-H⋯N(pyridine) in 3a,b or N3-H⋯N(pyridine) in 4a,b.

A detailed analysis of the crystal structures showed the conservative flat build of the central parts of all four studied compounds, despite the presence of different substituents at the C4 atom having the opposite functionality in hydrogen bonding (amine in 3a,b and oxygen atom in 4a,b). This difference concerns both the different hydrogen bonds of the two groups and the reversed positions of the pyridine ring, correlated with phenyl occupying another space. Still, due to conjugations and intramolecular hydrogen bonds, i.e., N4-H⋯N(pyridine) in 3a,b and N3-H⋯N(pyridine) in 4a,b, a rigid geometry of the central part of studied molecules was observed. One effect of the intramolecular hydrogen bonds in the case of 4 is the lack of another acidic H atom in the molecule which would be capable of interacting with a hypothetical receptor through a hydrogen bond. In addition, the hydrogen bond shields the carbonyl O atom from possible interactions as a hydrogen bond acceptor. However, in the case of 3a,b, one acidic H atom remains free for intermolecular interactions as a hydrogen bond donor, which should be kept in mind when comparing the differences in the activity profiles of hydrazonamides (3) and analogous hydrazides (4).

Another interesting observation was that structures 4a and its S-analog 4b (with thiophene instead of the furan ring) were not isostructural. However, a search of the Cambridge Structural Database [56] showed that among 170 crystal structures comprising one thiophene ring in a molecule (see Supplementary Materials for the fragments tested and details of the procedure), 35 examples (i.e., about 20%) of exact furan analogs were detected, of which almost half (16) were isostructural, i.e., they had the same space group, very similar unit cells dimensions, molecular conformation and packing. Therefore, the dissimilarity of the crystal structures of 4a and 4b did not seem unusual, especially given that both the S (in thiophene ring) and O (in furan ring) did not show any important interactions or close contact in the studied structures. Of course, in the case of compounds 3a and 3b, they were only formally S, O analogs. In fact, they are geometrical isomers and cannot form similar crystal structures.

3.6. Ab-Initio

Interestingly, 3a and 3b (differing only in the heteroatom in the five-membered ring) assumed completely different configurations (Figure 11) in the crystalline state. This observation prompted us to perform energy calculations for both compounds in unfolded (E isomer) and bent (Z isomer) forms. We optimized the geometry of the isolated molecules, taking into account the solvent (water) effect using the polarizable continuum model (PCM). The geometries of the optimized 3a (E) and 3b (Z) molecules differed very little from the original crystallographic conformations. It turned out that in both cases, the isomers found in the crystal states represented lower energy. For 3a, the energy of the unfolded (E) conformation was about 1.4 kcal/mol lower than that of the bent (Z) conformation. For 3b, the relationship was the opposite: the bent conformation had a lower energy, i.e., about 4.6 kcal/mol. The energy differences were not large, especially in the first case, but they suggest that the final step of reaction III (Figure 3) is under thermodynamic control.

4. Conclusions

In conclusion, four novel hydrazone derivatives of 4-phenylpyridine were successfully synthesized from methyl 4-phenylpicolinoimidate. Tests of antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and yeast showed that compound 3b, containing a nitrothiophene system in its structure and adopting a Z configuration at the N1=N2 bond, had the best activity against Gram-positive bacteria. Further studies are needed to evaluate the assumption that the promising profile of activity of 3b is related to its Z configuration at the N1=C2 bond. Additionally, significant potential activity of hydrazone derivative 4a with a nitrofuran system in its structure, assessed in vitro against M. tuberculosis, was detected.

The most important finding is that due to their significant potential action against the tested microorganisms, compounds 3b and 4a may be good starting structures for further research on the development of new antimicrobial drugs.