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Article

Stimulation of Metabolic Activity and Cell Differentiation in Osteoblastic and Human Mesenchymal Stem Cells by a Nanohydroxyapatite Paste Bone Graft Substitute

1
Unit of Biophysics and Bioengineering, School of Medicine and Health Sciences, University of Barcelona, Casanova 143, 08014 Barcelona, Spain
2
School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK
3
Ceramysis Ltd., 914 Herries Road, Sheffield S6 1QW, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Karla A. Batista and Kátia F Fernandes
Materials 2022, 15(4), 1570; https://doi.org/10.3390/ma15041570
Received: 23 December 2021 / Revised: 7 February 2022 / Accepted: 11 February 2022 / Published: 19 February 2022
(This article belongs to the Special Issue Bioactive and Functional Materials)
Advances in nanotechnology have been exploited to develop new biomaterials including nanocrystalline hydroxyapatite (nHA) with physical properties close to those of natural bone mineral. While clinical data are encouraging, relatively little is understood regarding bone cells’ interactions with synthetic graft substitutes based on this technology. The aim of this research was therefore to investigate the in vitro response of both osteoblast cell lines and primary osteoblasts to an nHA paste. Cellular metabolic activity was assessed using the cell viability reagent PrestoBlue and quantitative, real-time PCR was used to determine gene expression related to osteogenic differentiation. A potential role of calcium-sensing receptor (CaSR) in the response of osteoblastic cells to nHA was also investigated. Indirect contact of the nHA paste with human osteoblastic cells (Saos-2, MG63, primary osteoblasts) and human bone marrow-derived mesenchymal stem cells enhanced the cell metabolic activity. The nHA paste also stimulated gene expression of runt-related transcription factor 2, collagen 1, alkaline phosphatase, and osteocalcin, thereby indicating an osteogenic response. CaSR was not involved in nHA paste-induced increases in cellular metabolic activity. This investigation demonstrated that the nHA paste has osteogenic properties that contribute to clinical efficacy when employed as an injectable bone graft substitute. View Full-Text
Keywords: bone graft substitute; nanohydroxyapatite; osteoblastic cells; osteoblasts; osteogenic differentiation; calcium-sensing receptor; MSCs; cell activity bone graft substitute; nanohydroxyapatite; osteoblastic cells; osteoblasts; osteogenic differentiation; calcium-sensing receptor; MSCs; cell activity
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MDPI and ACS Style

Herranz-Diez, C.; Crawford, A.; Goodchild, R.L.; Hatton, P.V.; Miller, C.A. Stimulation of Metabolic Activity and Cell Differentiation in Osteoblastic and Human Mesenchymal Stem Cells by a Nanohydroxyapatite Paste Bone Graft Substitute. Materials 2022, 15, 1570. https://doi.org/10.3390/ma15041570

AMA Style

Herranz-Diez C, Crawford A, Goodchild RL, Hatton PV, Miller CA. Stimulation of Metabolic Activity and Cell Differentiation in Osteoblastic and Human Mesenchymal Stem Cells by a Nanohydroxyapatite Paste Bone Graft Substitute. Materials. 2022; 15(4):1570. https://doi.org/10.3390/ma15041570

Chicago/Turabian Style

Herranz-Diez, Carolina, Aileen Crawford, Rebecca L. Goodchild, Paul V. Hatton, and Cheryl A. Miller. 2022. "Stimulation of Metabolic Activity and Cell Differentiation in Osteoblastic and Human Mesenchymal Stem Cells by a Nanohydroxyapatite Paste Bone Graft Substitute" Materials 15, no. 4: 1570. https://doi.org/10.3390/ma15041570

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