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10.3390/ma15030993
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Article

Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

by
Mariko Yamamoto Kawai
1,2,*,
Ryosuke Ozasa
3,
Takuya Ishimoto
3,4,
Takayoshi Nakano
3,
Hiromitsu Yamamoto
2,
Marina Kashiwagi
2,
Shigeki Yamanaka
2,
Kazumasa Nakao
2,
Hiroki Maruyama
5,
Kazuhisa Bessho
2 and
Kiyoshi Ohura
6
1
Department of Welfare, Kansai Women’s College, Osaka 582-0026, Japan
2
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan
3
Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan
4
Center for Aluminum and Advanced Materials Research and International Collaboration, School of Sustainable Design, University of Toyama, Toyama 930-8555, Japan
5
Department of Clinical Nephroscience, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8501, Japan
6
Department of Nursing, Taisei Gakuin University, Osaka 587-8555, Japan
*
Author to whom correspondence should be addressed.
Materials 2022, 15(3), 993; https://doi.org/10.3390/ma15030993
Submission received: 26 November 2021 / Revised: 15 January 2022 / Accepted: 20 January 2022 / Published: 27 January 2022
(This article belongs to the Special Issue Materials for Hard Tissue Repair and Regeneration)
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Abstract

The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy.
Keywords: periodontal tissue; BMP; gene therapy; collagen micro-arrangement; bone quality periodontal tissue; BMP; gene therapy; collagen micro-arrangement; bone quality

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MDPI and ACS Style

Kawai, M.Y.; Ozasa, R.; Ishimoto, T.; Nakano, T.; Yamamoto, H.; Kashiwagi, M.; Yamanaka, S.; Nakao, K.; Maruyama, H.; Bessho, K.; et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials 2022, 15, 993. https://doi.org/10.3390/ma15030993

AMA Style

Kawai MY, Ozasa R, Ishimoto T, Nakano T, Yamamoto H, Kashiwagi M, Yamanaka S, Nakao K, Maruyama H, Bessho K, et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials. 2022; 15(3):993. https://doi.org/10.3390/ma15030993

Chicago/Turabian Style

Kawai, Mariko Yamamoto, Ryosuke Ozasa, Takuya Ishimoto, Takayoshi Nakano, Hiromitsu Yamamoto, Marina Kashiwagi, Shigeki Yamanaka, Kazumasa Nakao, Hiroki Maruyama, Kazuhisa Bessho, and et al. 2022. "Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer" Materials 15, no. 3: 993. https://doi.org/10.3390/ma15030993

APA Style

Kawai, M. Y., Ozasa, R., Ishimoto, T., Nakano, T., Yamamoto, H., Kashiwagi, M., Yamanaka, S., Nakao, K., Maruyama, H., Bessho, K., & Ohura, K. (2022). Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials, 15(3), 993. https://doi.org/10.3390/ma15030993

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