Next Article in Journal
Prediction of Kerf Width and Surface Roughness of Al6351 Based Composite in Wire-Cut Electric Discharge Machining Using Mathematical Modelling
Next Article in Special Issue
Numerical Simulation of Failure Behavior of Brittle Heterogeneous Rock under Uniaxial Compression Test
Previous Article in Journal
Evolution of Intrinsic and Extrinsic Electron Traps at Grain Boundary during Sintering ZnO Based Varistor Ceramics
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Materials
Volume 15
Issue 3
10.3390/ma15031101
materials-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Biodegradable Thermoplastic Starch/Polycaprolactone Blends with Co-Continuous Morphology Suitable for Local Release of Antibiotics

by
Veronika Gajdosova
1,
Beata Strachota
1,
Adam Strachota
1,
Danuse Michalkova
1,
Sabina Krejcikova
1,
Petr Fulin
2,
Otakar Nyc
3,
Adam Brinek
4,
Marek Zemek
4 and
Miroslav Slouf
1,*
1
Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Heyrovsky Sq. 2, Prague 6, 162 06 Prague, Czech Republic
2
1st Orthopedic Clinic, 1st Faculty of Medicine, Charles University, Motol University Hospital, V Uvalu 84, Prague 5, 150 06 Prague, Czech Republic
3
Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University, Motol University Hospital, V Uvalu 84, Prague 5, 150 06 Prague, Czech Republic
4
CEITEC—Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, 612 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Materials 2022, 15(3), 1101; https://doi.org/10.3390/ma15031101
Submission received: 25 December 2021 / Revised: 21 January 2022 / Accepted: 28 January 2022 / Published: 30 January 2022
(This article belongs to the Special Issue Relations between Structure, Micro- and Nanomechanical Properties of Materials)
Browse Figures
Review Reports Versions Notes

Abstract

:
We report a reproducible preparation and characterization of highly homogeneous thermoplastic starch/pol(ε-caprolactone) blends (TPS/PCL) with a minimal thermomechanical degradation and co-continuous morphology. These materials would be suitable for biomedical applications, specifically for the local release of antibiotics (ATB) from the TPS phase. The TPS/PCL blends were prepared in the whole concentration range. In agreement with theoretical predictions based on component viscosities, the co-continuous morphology was found for TPS/PCL blends with a composition of 70/30 wt.%. The minimal thermomechanical degradation of the blends was achieved by an optimization of the processing conditions and by keeping processing temperatures as low as possible, because higher temperatures might damage ATB in the final application. The blends’ homogeneity was verified by scanning electron microscopy. The co-continuous morphology was confirmed by submicron-computed tomography. The mechanical performance of the blends was characterized in both microscale (by an instrumented microindentation hardness testing; MHI) and macroscale (by dynamic thermomechanical analysis; DMTA). The elastic moduli of TPS increased ca four times in the TPS/PCL (70/30) blend. The correlations between elastic moduli measured by MHI and DMTA were very strong, which implied that, in the future studies, it would be possible to use just micromechanical testing that does not require large specimens.

1. Introduction

Due to environmental and sustainability issues, biodegradable polymers have attracted more and more attention [1,2]. The most widely used biodegradable polymers are starch and poly (lactic acid) [3]. Starch is popular due to its natural origin, abundance connected with a low cost, excellent biocompatibility, and complete biodegradability [4]. Natural starch is a white, non-plastic powder which decomposes before melting [4,5]. This natural powder must be plasticized so that it can be treated by classical polymer processing techniques such as melt-mixing or compression molding [6,7,8,9]. The starch plasticization (or gelatinization) consists of mixing starch powder with low molecular weight compounds such as water, glycerol, citric acid, etc. [10,11,12], and results in thermoplastic starch (TPS). Two main ways of starch plasticization are solution casting (SC; [13,14] and melt-mixing (MM; [6,10,15]). A series of our studies [16,17,18] proved that highly homogenous TPS (i.e., material without microscale inhomogeneities, suitable not only for technical, but also for biomedical applications) is best obtained by a two-step preparation procedure, which comprises SC followed by MM.
In most applications, TPS is used in the form of blends with other polymers [19]. The reason is that neat TPS is rather soft, very sensitive to moisture, and its biodegradability rate is too high [4,20]. Therefore, most authors blended TPS with other biodegradable polymers such as polylactic acid (PLA) [21,22,23], poly(ε-caprolactone) (PCL) [10,23,24,25], and some others, such as poly (butylene adipate-co-terephthalate (PBAT) [7] or polyvinyl alcohol (PVA) [26]. By far the most popular are TPS/PCL blends, which are even produced commercially by at least two big manufacturers: the Novamont company produces TPS/PCL blends named Mater-Bi [27,28] and the Bioplast company produces TPS/PCL blends with trademark Biotec® [29]. Binary TPS/PCL blends aim for technical applications, namely in the field of packaging [30,31,32]. As for biomedical applications, the TPS/PCL particles in solution have been considered as possible microcapsules for drug delivery [33,34], but bulk solid TPS/PCL blends have been mentioned in just two studies: Bou-Francis et al. [24] investigated TPS/PCL together with other biodegradable polymer blends as potential fracture fixation devices (which is suspicious due to the very low modulus of PCL/TPS blends) and Mano et al. [25] studied the thermal properties of TPS, which was blended with various synthetic biodegradable polymers including PCL, and concluded that all starch-based blends might be suitable for some medical applications.
This work deals with a reproducible preparation of highly homogeneous TPS/PCL blends for a local release of antibiotics (ATB). The fully biodegradable TPS/PCL/ATB systems are being developed in collaboration with a local hospital for the treatment of strong local infects, such as osteomyelitis [35]. The basic idea has been described in our patent [36]: starch is mixed with ATB easily during our optimized plasticization procedure [16], the resulting TPS/ATB system is melt-mixed with PCL, and the final TPS/PCL/ATB system releases ATB at a rate that is controlled by the blend composition and morphology. More details concerning the biomedical applications of TPS/PCL/ATB systems are summarized in Appendix A, while this work is focused on the optimization of the TPS/PCL preparation procedure, which should yield highly homogeneous TPS/PCL blends with co-continuous morphology and with the minimal thermomechanical degradation of the system. The perfectly homogeneous material without local fluctuations is required due to the target application, which requires well-defined ATB release. The co-continuous morphology of TPS/PCL blends is necessary for fast ATB release from the TPS phase, as documented in our patent [36]. The minimal thermomechanical degradation (i.e., low processing temperatures and low torque forces during starch plasticization and melt-mixing) is extremely important in order to keep the bacteriostatic and bacteriocidic activity of the incorporated antibiotics, as indicated by our parallel experiments (preliminary results for neat TPS/ATB systems have already been published [18]).

2. Materials and Methods

2.1. Materials

Wheat starch powder (type A) was supplied by Škrobárny Pelhřimov, a.s. (Pelhřimov, Czech Republic). The granular poly(ε-caprolactone) Capa 6800 (PCL; M = 80,000 g·mol−1, Tm = 58 °C) was supplied by Perstorp Group (Malmö, Sweden). Anhydrous glycerol (>99%), hydrochloric acid (HCl; 35%), and sodium bromide (NaBr; >99%) were purchased from Lach-Ner, s.r.o. (Neratovice, Czech Republic).

2.2. TPS/PCL Blend Preparation

The TPS/PCL blends were prepared in two ways. In either case, the first step of our preparation was the plasticization of starch (Section 2.2.1). The second step was either melt-mixing of TPS with PCL (the two-step preparation procedure; Section 2.2.2) or additional melt-mixing and homogenization of TPS followed by the final melt-mixing of TPS and PCL (the three-step preparation procedure; Section 2.2.3). The overall schemes of both preparation procedures are given in the Supplementary Materials Section. All prepared TPS/PCL samples are listed in Table 1. All prepared samples were stored in a wet desiccator with a saturated solution of sodium bromide (Rh = 57%) between the experiments.

2.2.1. Preparation of TPS by Solution Casting

The solution casted (SC) TPS was prepared according to Ostafińska et al. [16]. The wheat starch (70 wt.%) with glycerol (30 wt.%) and distilled water (6 parts of water per 1 part of starch) were premixed with a magnetic stirrer in a beaker for 30 min at room temperature. The premixed material was transferred to a mechanical stirrer, where it was mixed at elevated temperature (at least 10 min at temperature 65–75 °C until the viscosity significantly increased). The gelatinized starch was spread on a PE foil (in the form of ~1–2 mm thick film) and dried at room conditions (temperature 25 °C, relative humidity ~40%) for 3 days.

2.2.2. Two-Step Preparation of TPS/PCL Blends

In the two-step preparation procedure, the solution casted TPS (step 1) was homogenized with PCL by melt-mixing (MM; step 2) at elevated temperature in the W50EH chamber of the twin-screw laboratory mixer (Brabender Plasti-Corder; Duisburg, Germany). The samples were mixed in the chamber preheated to 120 °C, using rotation speed 60 rpm for 8 min. The blend was compression molded to 2 mm thick plaques in a laboratory hot press (Fontijne Grotnes; Vlaardingen, Netherlands) at 130 °C for 2 min under 50 kN to deaerate, plus another 1 min under 100 kN followed by water cooling for ca 15 min under 100 kN to obtain the final plaques.

2.2.3. Three-Step Preparation of TPS/PCL Blends

In the three-step preparation procedure, the solution casted TPS (step 1) was further homogenized by melt-mixing (step 2) and was then blended with PCL (step 3). The conditions of melt-mixing during steps 2 and 3 (120 °C and 60 rpm for 8 min) were the same as in the two-step procedure described above (Section 2.2.2). The final compression molding conditions were identical to the two-step procedure as well.

2.3. TPS/PCL Blend Characterization

2.3.1. Rheology

Rheological properties were measured on a strain-controlled ARES-G2 rheometer (TA Instruments, New Castle, DE, USA) using a parallel plate fixture with a diameter of 30 mm (plates with cross-hatched surface to prevent slipping). Frequency sweep experiments were performed in a frequency range from 0.1 to 100 rad/s at a strain amplitude of 0.05%, and at a constant temperature of 120 °C. The linear viscoelasticity region was determined in view of the dependence of the storage modulus on the strain amplitude, which was measured at 120 °C at a frequency of 1 Hz. The thermal stability of the materials at 120 °C was evaluated in time sweep experiments. To ensure a uniform temperature, all samples were equilibrated for 2 min prior to the start of each type of experiment.

2.3.2. Morphology

The morphology of the TPS/PCL blends was visualized using the scanning electron microscopy (SEM) using a microscope MAIA3 (Tescan, Brno, Czech Republic). All samples were fixed on a metallic support with a conductive silver paste (Leitsilber G302; Christine Groepl, Tulln, Austria), sputter-coated with a thin platinum layer (vacuum sputter coater SCD 050; Leica, Austria; thickness of the Pt layer: approx. 4 nm), and observed by means of secondary electron imaging at accelerating voltage 3 kV.
For each blend composition, we observed two types of specimens: (i) smoothed and etched surfaces revealed the phase morphology of the samples and (ii) fracture surfaces documented interfacial adhesion between the components. The smooth surfaces were prepared as described elsewhere [35], etched with 6N HCl, washed out with distilled water and dried at room temperature. The etching conditions were adjusted to TPS/PCL blend composition: the blends with the PCL matrix or co-continuous phase (i.e., from TPS/PCL (10/90) to TPS/PCL (70/30) were etched for 10 min in the 6N HCl and the remaining two blends with TPS matrix (i.e., 80/20 and 90/10) were etched in the 6N HCl for only 30 s. The fracture surfaces were prepared through the breaking of the samples submerged in liquid nitrogen [37]. Before the SEM analyses, the samples were placed for 24 h in a dry desiccator (with silica gel), which improved their stability during the vacuum sputter-coating process (minimization of artefact cracks on the surface).

2.3.3. Phase Co-Continuity

Co-continuity of the TPS/PCL (70/30) blend was investigated through laboratory submicron-computed tomography (subμ-CT) using a nano3DX X-ray microscope (Rigaku Corporation, Tokyo, Japan). Part of the sample was cut down to fit in a field of view (FoV) of roughly 0.9 × 0.9 × 0.7 mm3. The cut piece was inserted into a Kapton tube and affixed to the walls of the tube using superglue; the tube was then placed on a sample holder and sealed using hot glue. Next, 800 projections of the sample were obtained over a 180° range, using an X-ray generator equipped with a Cu target, with a tube voltage of 40 kV and a current of 30 mA. Exposure per projection was 22 s. A phase retrieval algorithm [38] was applied for contrast enhancement (with a δ/β parameter of 300) and finally an in-house reconstruction algorithm was used to obtain a ~3.4 gigapixel CT volume with a voxel size of 0.528 µm. The volume was processed in VG Studio Max 3.5 (Volume Graphics, Heidelberg, Germany). The hydrogel sample was segmented from its background using ISO50 [39], followed by the manual removal of residual segmented debris and CT artifacts near the edges of the FoV. A region of interest (RoI) was formed around the etched region of the segmented sample and porosity analysis was conducted in this RoI using the “Only threshold” tool of VG Studio.

2.3.4. Micromechanical Properties

Micromechanical properties were obtained from instrumented microindentation hardness testing (MHI) performed with a microindentation combi tester (MCT tester; CSM Instruments, Corcelles, Switzerland). We performed quasi-static measurements with Vickers indenter: a diamond square pyramid with an angle between nonadjacent faces 136° was forced against a smooth sample surface with the following parameters: loading force 981 mN (=100 gf), dwell time (time of maximal load) 60 s, and linear loading and unloading rates 12,000 mN/min (total loading and unloading time ≈ 5 s). The smooth surfaces for MHI testing were prepared with a rotary microtome RM 2155 (Leica, Vienna, Austria). For each sample, at least three independent cut surfaces were prepared and at least 10 indentations were carried out per surface (i.e., each sample was measured at least 30 times) and the final results were averaged. Five micromechanical properties were obtained from F–h curves (where F is the loading force and h is the indenter penetration depth) according to ISO 14577-1 standard: indentation modulus (EIT), indentation hardness (HIT), Martens hardness (HM), indentation creep (CIT), and elastic part of indentation work (ηIT). Figure 1 shows the principle of MHI measurement and evaluation in the case of our TPS/PCL blends; further details can be found in our recent papers [40,41].

2.3.5. Macromechanical Properties

Temperature-dependent dynamic–mechanical properties of the prepared blends were tested on rectangular specimens (40 × 10 × 2 mm) using the same rheometer usedfor the rheological characterization. An oscillatory shear deformation at a constant frequency of 1 Hz and a heating rate of 3 °C/min were applied. The shear deformation was kept in the range from 0.01 to 4% during the temperature sweep, using the AutoStrain function, in order to keep the torque value in the proper range. The evaluated temperature range was from −90 °C to 170 °C.

3. Results and Discussion

3.1. Relations between Preparation, Rheology and Morphology of TPS/PCL Blends

The main objective of this work was to find a reproducible preparation procedure for highly homogeneous TPS/PCL blends with a co-continuous morphology and minimal thermomechanical degradation of the system during preparation. Blends with these features are required for the release of antibiotics from TPS phase, as explained in the Introduction. To obtain the desired systems, we compared two preparation procedures (Section 3.1.1), considered rheological properties (Section 3.1.2), and characterized the final TPS/PCL morphology by both 2D imaging using a scanning electron microscopy (Section 3.1.3) and 3D imaging using a submicron-computed tomography (Section 3.1.4).

3.1.1. Two-Step vs. Three-Step Preparation Procedure

The advantage of the two-step preparation procedure (Section 2.2.2) is the shorter thermomechanical treatment of TPS (which should contain ATB in the final application), while the three-step preparation procedure (Section 2.2.3) is expected to yield more homogeneous morphology. The first and the last step of both procedures (starch plasticization and the final melt-mixing) were identical. The difference consisted in the intermediate starch homogenization in the three-step procedure. Unfortunately, the melt-mixing of neat TPS exhibited very high torque moments connected with an increase in temperature inside the mixing chamber (from the preset of 120 °C up to 135 °C), while the addition of PCL mitigated these negative effects significantly (as illustrated in Figure 2). This unwanted temperature increase may be decisive, because the two most common antibiotics employed in the treatment of osteomyelitis—vancomycin and gentamicin [35]—are claimed to be stable, just slightly above 120 °C [42,43,44,45]. The decrease in torque moments and in-chamber temperatures during the melt-mixing of TPS/PCL systems as a function of PCL concentration were connected with the much lower viscosity of PCL in comparison with TPS (as discussed in the following Section 3.2.1). In any case, we concluded that the benefit of the three-step preparation (a slightly finer morphology, as illustrated in Figure A1 in Appendix A) did not outweigh the main disadvantage (the higher thermomechanical load of TPS connected with the increased risk of ATB degradation in the final application). Consequently, all following experiments in this study were based on TPS/PCL blends prepared by a two-step preparation procedure.

3.1.2. Rheology of TPS/PCL Blends

Figure 3 summarizes the main output of the oscillatory shear measurements: the frequency dependence and the viscosity (more precisely: of the absolute value of the complex viscosity, |η*|) of all TPS/PCL blends at 120 °C. The results documented two general trends: (i) the viscosity of PCL was much lower than the viscosity of TPS, although the difference somewhat decreased with the increasing frequency and (ii) the addition of PCL to TPS decreased the viscosity of the system, although the situation was more complex at low concentrations of PCL, as discussed below.
For the blend compositions close to the neat components, i.e., for the blends TPS/PCL (100/0 and 90/10) and TPS/PCL (0/100, 10/90, and 20/80), the complex viscosity curves in Figure 3 stayed close to those characteristic of the neat constituent phases. The slight increase in the blend viscosity with respect to the neat constituent is typical of the particulate blends with good interfacial adhesion, as summarized elsewhere [46]. The highest viscosity was observed for the TPS/PCL (80/20) blend with high-viscosity matrix and PCL minority phase. The blends with an even higher content of PCL, i.e., the blends from TPS/PCL (70/30) to TPS/PCL (30/70), exhibited a gradual decrease in viscosity, which indicated the formation of the continuous structure of PCL. This was in perfect agreement with the results of SEM (Section 3.1.3.) and subμ-CT (3.1.4), which confirmed that the blends with TPS content ≥30 wt.% contained a continuous PCL phase. The steepest viscosity decrease was observed for the blends with 30 and 40 wt.% of TPS. The comparison between the values of storage and the loss moduli (Appendix B, Figure A2) suggested that the blends with TPS content above 30 wt.% behaved like physical gels (G′ > G″), while the blends with TPS content below 30 wt.% exhibited liquid-like behavior (G′ < G″) at a given temperature and experimental conditions [47].
The good interfacial adhesion between TPS and PCL, suggested by the rheological experiments, was confirmed by SEM micrographs that showed the fracture surfaces of TPS/PCL blends (Appendix B, Figure A3). The good adhesion could be attributed to the affinity of both phases to each other (hydrogen bridging between hydroxyl groups of starch and carboxyl groups of PCL). The absolute value of complex viscosity (η*), in oscillatory shear experiments at a given frequency (ω), is proportional to the absolute value of complex modulus (|G*| = ω ∙ |η*|; [47]), which represents the overall resistance of the investigated system to deformation. The high resistance to the deformation of the TPS/PCL blends with 20–40 wt.% of PCL (manifested by high |η*|) could only be observed in oscillatory experiments, where the applied deformations were small (within the linear viscoelastic region). During the real melt-mixing of the blends, the shear forces were higher, the chemical bridges between both phases were broken, and the lower-viscosity PCL component acted as a lubricant, which decreased the torque moments and processing temperatures in the whole concentration range, in agreement with the experimental observations described above (Section 3.1.1).

3.1.3. Morphology of TPS/PCL Blends

Morphology of TPS/PCL blends was visualized by SEM microscopy (Figure 4). The blends with higher concentrations of PCL (Figure 4a–e) contained TPS particles in the PCL matrix. The size of the TPS particles decreased with the increasing concentration of TPS component, although most blends exhibited the opposite trend—bigger particles at higher concentrations of the minority components [48,49,50]. This nontypical feature of TPS/PCL systems resulted from the much higher viscosity of TPS in comparison with PCL at the processing temperature of 120 °C (as documented in Figure 3).
At low TPS concentrations, the highly viscous and compact TPS particles could not be fragmented in the low-viscosity PCL matrix. At higher TPS concentration, the overall viscosity of the system increased (Figure 3), which resulted in higher shear forces during melt-mixing that caused a more frequent particle breakup and the structure became finer. The blends with higher concentration of TPS showed a co-continuous morphology (Figure 4f,g) and, finally, the reversed morphology of the TPS matrix containing PCL particles (Figure 4h,i). The reason why the co-continuous morphology region of the TPS/PCL blends was shifted from 50/50 concentration and the proof of the TPS phase co-continuity of TPS/PCL (70/30) blend are given in the Section 3.1.4.

3.1.4. Phase Co-Continuity of TPS/PCL Blends

The theoretical prediction and experimental verification of the phase co-continuity in TPS/PCL blends are shown in Figure 5. The prediction of phase co-continuity was based on the classical analysis of Paul and Barlow [51], which we summarize in the form of a simple scheme (Figure 5a). Briefly, point (1) in Figure 5a shows that the co-continuous morphology of a binary polymer blend occurs at 50/50 composition (volume fraction ratio = 1) on the condition that the viscosities of the individual components are equal (viscosity ratio = 1). Nevertheless, in our case, the TPS/PCL viscosity ratio was significantly higher than 1 (point (2) in Figure 5a), and, consequently, the phase co-continuity was observed at higher TPS/PCL volume ratios of 60/40 and 70/30 (Figure 4 and point (3) in Figure 5a). The proof of phase co-continuity was obtained by submicron-computed tomography (subμ-CT; Figure 5b). Analysis of subμ-CT data showed that the etching of the TPS/PCL (70/30) blend, for which the two-dimensional SEM micrographs (Figure 4) indicated the highest degree of co-continuity, removed the TPS phase up to a depth of about 100 µm. Porosity analysis revealed that the etched volume forms a single interconnected network spanning the entire width and depth of the etched region. This network makes up about 95.34% of the total porosity identified in the RoI, confirming the hypothesized high degree of co-continuity in the sample. The true degree of co-continuity may be even higher when accounting for pores at the edges of the RoI and pores connected by channels which could not be resolved at the spatial resolution of the CT measurement.

3.2. Micro- and Macromechanical Properties of TPS/PCL Blends

The second objective of this work was the characterization of the mechanical behavior of TPS/PCL blends in order to verify whether the soft TPS phase was stiffened by the addition of PCL. Moreover, we wanted to describe the mechanical performance of all blends in both microscale (using MHI; Section 3.2.1) and macroscale (using DMTA; Section 3.2.2). The microscale characterization would be advantageous for future analyses of TPS/PCL/ATB systems, which are usually prepared in low amounts due to costly antibiotics. Therefore, we quantified the correlations between the corresponding properties from MHI and DMTA measurements to check if the microscale characterization yields relevant results (Section 3.2.3).

3.2.1. Micromechanical Properties

Instrumented microindentation hardness testing (MHI) yielded five micromechanical properties of TPS/PCL blends: indentation modulus (EIT; proportional to macroscopic moduli), indentation hardness (HIT; proportional to macroscopic yield stress), indentation creep (CIT; related to macroscopic creep), elastic part of the indentation work (ηIT; connected with overall elasticity of the sample), and Martens hardness (HM; analogous to HIT, but calculated independently on the Oliver and Pharr theory [52]). Figure 6 shows the first four micromechanical properties (EIT, HIT, CIT, and ηIT) plotted as a function of the TPS/PCL composition and compared with two predictive models (linear model, LIN, and equivalent box model, EBM). The LIN model, which is also referred as rule-of-mixing or additivity law, assumes that the arbitrary property P of a multicomponent system is a linear combination of its component properties:
P = ∑ i v i P i
where Pi and vi represent a given property and volume fraction of i-th component, respectively. The simple LIN model holds surprisingly well in some cases, such as the elastic moduli of polymer composites with infinite fibers [50,53] or microhardness of semicrystalline polymers [54,55]. Nevertheless, for polymer blends, the LIN model is just a rough approximation, representing the upper achievable limit of the real blend properties [55,56]. The more advanced EBM model, which describes the selected properties of isotropic binary blends, also takes into account the degree of phase co-continuity and interfacial adhesion [57]. In terms of EBM, the indentation modulus (EIT) and hardness (HIT) of a binary polymer blend are defined by Equations (2) and (3), respectively:
E I T = E 1 v 1 p + E 2 v 2 p + v s 2 / v 1 s / E 1 + v 2 s / E 2
H I T = H 1 v 1 p + H 2 v 2 p + A H 1 v s
where Ei and Hi are the component properties and vij represents volume fractions (subscript (i) identifies the component and subscript (j) determines the volume of the component in the parallel (p) and serial (s) branch of the EBM model, which correspond to volume fractions with continuous and particulate morphology, respectively). The parameter A represents interfacial adhesion (the values A = 0 and 1 mean negligible and perfect adhesion, respectively). The details of the EBM model were described in the original work of Kolarik [57] and have also been summarized in our recent studies [50,58]. It is worth noting that the EBM model was derived for macroscopic elastic modulus (E) and yield stress (Y), but it has been demonstrated that EBM also predicted micromechanical properties EIT ≈ E [52] and HIT ≈ 3 Y [59]. The volume fractions (vij) in the EBM model can be either estimated from additional experiments (for example, from SEM micrographs [48]) or calculated theoretically (from percolation theory [57]), while the adhesion parameter (A) is obtained from the fitting of experimental data (hardness or yield stress as a function of composition; Equation (3), ref. [48,60]).
In this work, the EBM predictions for EIT and HIT (Figure 6A,B) were based on theoretically calculated vij values (which were shown to be a good first approximation in many binary polymer blends [48,57,58,60]) and maximal interfacial adhesion (A = 1). The fact that both EIT and HIT exceeded the theoretical EBM predictions evidenced strong interfacial adhesion and very good compatibility between TPS and PCL, which was is in agreement with the rheological and morphological results (Section 3.1.2 and Section 3.1.3). The two supplementary properties, CIT and ηIT (Figure 6C,D), were in reasonable agreement with LIN predictions, which reconfirmed the good compatibility of TPS and PCL polymers, because LIN model represents the general upper achievable limit of blend properties, as discussed above.

3.2.2. Macromechanical Properties

The macroscale thermomechanical properties of the TPS/PCL blends were analyzed by oscillatory torsion experiments (DMTA). The DMTA results are shown in Figure 7 in the form of storage moduli (G′) as a function of temperature (G′ = f (T) curves). The complete set of DMTA properties (storage, loss, and complex moduli, together with damping factors) in a temperature range from −80 °C to 160 °C is shown in Appendix C (Figure A6).
In the high temperature region (from ~60 °C to 160 °C), the blends with the TPS content ≥30 wt.% behaved like elastic gels (approximately constant values of G′ above 60 °C), while the blends with the TPS content ≤20 wt.% behaved like polymer melts (a steep decrease of G′ above 60 °C). This agreed very well with the rheological measurements performed at 120 °C (Section 3.1.2), which proved that the TPS/PCL blends with TPS ≥ 30 wt.% exhibited a mostly solid-like character (G′ > G″ in Figure A3), whereas TPS/PCL blends with lower TPS content showed a liquid-like character (G″ > G′ in Figure A3). As the temperature of 120 °C was approximately equal to the processing temperature (Figure 2), the above-discussed DMTA results were also connected with the TPS/PCL behavior during melt-mixing. The strong decrease of G′ around 60 °C corresponded to the melting temperature of PCL.
In the medium temperature region (from ca 5 °C to 60 °C), the storage moduli of TPS/PCL blends increased monotonously with the PCL content. This was connected with the fact that TPS started to soften above −10 °C, while PCL softened by melting above 60 °C (and so it was stiffer than TPS at medium temperatures). The changes in shape of the DMTA curves corresponded approximately to the weighted sum of the blend components, which is typical for immiscible blends. The inset in Figure 7 shows the G′ values around the room temperature of 25 °C, where the storage modulus decreased by 1 order of magnitude if going from neat PCL (ductile semicrystalline polymer above Tm with G′ ~150 MPa) to neat TPS (soft rubber with G′ ~16 MPa). The medium temperature region and especially the temperatures around 25 °C were important for the correlations between the results of macroscale DMTA testing and microscale MHI testing (which are discussed in the Section 3.2.3) because the MHI measurements were performed at room temperature.
In the low temperature region (temperatures below 5 °C), both blend components exhibited glass transition temperatures (Tg) and the behavior of the TPS/PCL systems changed accordingly. Neat PCL showed Tg at −55 °C (a step of the G′ = f(T) curve; an even larger step was observed at the PCL melting temperature around 60 °C). Neat TPS showed two Tg temperatures, corresponding to two steps of the G′ = f(T) curve: the first step was around −57 °C (overlapping with the Tg of PCL) and the second was a very broad transition at around 0 °C (the onset of the step in Figure 7 at −10 °C and the end of the step at 40 °C). In accordance with the previous studies [61,62], the lower Tg of TPS was assigned to the glycerol-rich phase, while the higher Tg was assigned to the starch-rich phase. As expected, the glass transition temperatures could also be observed in the form of peaks at G″ = f(T) and tan(δ) = f(T) curves (Appendix C, Figure A6). As for TPS/PCL blends, the rising TPS content led to higher moduli in the fully vitrified state below −80 °C, to higher moduli in the rubbery plateau above 60 °C, to a diminishing melting step of PCL at 60 °C, and to a more prominent glass transition of TPS observed near 2 °C (as documented in both Figure 7 and Figure A6 in Appendix C).

3.2.3. Correlations between Micro- and Micromechanical Properties

The mechanical properties measured in this work can be divided into two groups: stiffness-related properties (G′, EIT, HIT, and HM) and viscosity-related properties (G″, tan(δ), CIT, and ηIT). The same division of mechanical properties was employed and justified in our previous work [40]. The stiffness-related properties were more important here because the soft TPS matrix was expected to harden thanks to the PCL addition. As noted in the previous paragraph, all correlations between micro- and macromechanical properties were evaluated for the room temperature of 25 °C.
The correlations between all stiffness-related properties were very strong, as evidenced in Figure 8. The fact that the macromechanical storage modulus (G′) was strongly correlated with all stiffness-related micromechanical properties (EIT, HIT and HM) proved that the micromechanical characterization of the TPS/PCL blends was reliable and suitable for ongoing parallel studies with lower volumes of the blends containing antibiotics. Moreover, the strong correlation between the first two micromechanical properties (EIT and HIT) evaluated in terms of the Oliver and Pharr theory (O & P theory; [52]) and the last property (Martens hardness, HM), which is evaluated directly from the experimental data [63], evidenced that the O & P theory is a good approximation in the case of TPS/PCL blends. The same trend of HIT and HM dependencies on the blend composition is also evident in Figure A5 from Appendix C.
The correlations between viscosity-related properties were somewhat weaker, but identifiable. Microscale properties (CIT and ηIT) showed quite strong and logical trends, as illustrated in Figure 6: the increasing concentration of the soft and viscous TPS component resulted in lower creep resistance (increasing CIT; Figure 6C) and lower elasticity (decreasing ηIT; Figure 6D). Macroscale properties related to viscosity (G″ and tan(δ)) changed with the increasing concentration of TPS in two different ways, as documented in Figure A5: the values of G″ at 25 °C were similar for all TPS/PCL blends (Figure A5B) without a strong trend, but the values of tan(δ) at 25 °C increased clearly and monotonously with the increasing TPS concentration (Figure A5D). Considering that the shear moduli G′, G″, and |G*| represent the deformation energy stored due to elasticity, deformation energy lost due to viscous flow, and total material resistance to deformation, respectively, while the damping factor tan(δ) = G″/G′ represents ratio of the viscous and elastic portion of the deformation behavior [47], the final interpretation is as follows: For increasing the concentration of TPS in the TPS/PCL blends at 25 °C, the overall elasticity of the system decreased (Figure A5A), the overall viscosity was roughly constant (Figure A5B), the total resistance to deformation decreased as it was dominated by the elastic contribution G′ (similar trends in Figure A5A,C), and the viscous behavior played an increasingly important role (Figure A5D), which was quite reasonable as the TPS represented the softer and less elastic component of the blend. All correlations are summarized in Appendix C in the form of a heatmap (Figure A7).

4. Conclusions

We have described a reproducible preparation of highly homogeneous TPS/PCL blends and characterized their properties in both macro- and microscale. The biodegradable TPS/PCL systems, containing antibiotics in TPS phase, are being developed for the treatment of strong infects within parallel collaboration with a local hospital. This work was focused on the optimization of the TPS/PCL preparation procedure, which should yield perfectly homogeneous TPS/PCL blends with a co-continuous morphology and minimal thermomechanical degradation of the system. The perfectly homogeneous material is necessary for reproducible and well-defined ATB release. The co-continuous morphology of TPS/PCL blends is required for fast ATB release from the fast-degrading TPS phase. The minimal thermomechanical degradation during the preparation procedure is essential in order to keep the high activity of the antibiotics in the final application. The main conclusions of the current study can be summarized as follows:
1.
The two-step preparation procedure, comprising the preparation of TPS by solution casting and melt-mixing of TPS with PCL, yielded highly homogeneous TPS/PCL blends reliably and reproducibly;
2.
The TPS/PCL (70/30) blends exhibited co-continuous morphology and the processing temperature during the preparation should be kept below 130 °C due to the addition of the less viscous PCL phase;
3.
The TPS/PCL blends showed high interfacial adhesion and their stiffness-related properties (elastic moduli and hardness) were even slightly above the theoretical predictions based on the EBM model. In other words, the very soft TPS matrix could be stiffened quite efficiently by means of the compatible PCL component;
4.
The mechanical properties of the TPS/PCL blends were characterized in both macroscale (by DMTA) and microscale (by MHI). The correlations between corresponding macro- and micromechanical properties were strong. This confirmed that micromechanical measurements were quite sufficient for the characterization of the mechanical performance of the TPS/PCL blends. Consequently, the future mechanical characterizations of the TPS/PCL/ATB systems, which are usually available in lower volumes due to costly antibiotics, could be performed simply by microindentation hardness testing, which does not require large specimens.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/ma15031101/s1: (i) The table containing the values and standard deviations of all micromechanical and macromechanical properties of the TPS/PCL blends (the blends prepared by the two-step preparation procedure; measurements performed at 25 °C). (ii) The overall scheme of TPS/PCL blend preparation by both two-step and three-step preparation procedure.

Author Contributions

Conceptualization, M.S. and P.F.; validation, M.S. and V.G.; formal analysis, M.S., V.G., B.S., A.S., S.K., A.B. and M.Z.; investigation, V.G., B.S., D.M., S.K, A.B.; resources, M.S., A.S., D.M. and A.B.; data curation M.S., V.G., B.S., A.B. and M.Z.; writing—original draft preparation, M.S., V.G., A.S. and M.Z.; writing—review and editing, M.S.; visualization, M.S. and V.G.; supervision, M.S., P.F. and O.N.; project administration, M.S., P.F. and O.N.; funding acquisition, M.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Technology Agency of the Czech Republic (TA CR), grant number TN01000008, Czech Health Research Council (AZV CR), grant number NU21-06-00084, Czech Science Foundation (GA CR), grant number 19-04925S, and Ministry of Youth, Health and Sports of the Czech Republic (MEYS CR), grant number LM2018110.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are available at request to the corresponding author.

Acknowledgments

The authors thank to Tomas Zikmund from CEITEC for useful discussions and help with interpretation of 3D data and to Roman Sticha from Motol University Hospital for his help with supplementary microbiological experiments.

Conflicts of Interest

The authors declare no conflict of interest.

Appendix A. Biomedical Applications of PCL/TPS Blends

This work focuses on reproducible preparation and characterization of highly homogeneous TPS/PCL blends with co-continuous morphology. Nevertheless, as mentioned in the Introduction and in our patent [36], the TPS/PCL blends are suitable for the local release of antibiotics. Although the biomedical applications of the blends are not the subject of this study, selected issues concerning the local release of antibiotics are clarified below:
Both TPS and PCL are biocompatible and biodegradable polymers. The biocompatibility of both polymers is well documented [1,64]. The biodegradability rate of TPS/PCL blends in vivo is given by PCL, because the TPS component degrades much faster (within a few days) than PCL (ca 2–4 years) [65,66,67].
The TPS/PCL/ATB systems (where ATB = suitable antibiotic dispersed in TPS phase) can be employed in the treatment of strong local infections such as osteomyelitis, which is an infection of bone tissue. The two major antibiotics for osteomyelitis treatment are vancomycin and gentamicin [35]. Both substances exhibit reasonable resistance to thermal degradation [42,43,44,45] and, as a result, they can survive the TPS/PCL preparation procedure and keep their activity, as we have already documented by standard microbiological tube-dilution tests [18]. The tests were performed in our initial study about simplified TPS/ATB systems [18] and also in our parallel experiments with TPS/PCL/ATB systems (unpublished results, illustrated in Figure A1).
Materials 15 01101 g0a1 550
Figure A1. The results of standard microbiological tube dilution tests, in which a defined piece of TPS/PCL/ATB sample (ATB dispersed in TPS phase) was put into a tube containing 5 mL of Mueller-Hinton broth (Oxoid, Brno, Czech Republic); each tube is then inoculated with a standardized bacterial suspension of the CCM 4223 Staphylococcus aureus and observed after 24 h. The turbid solution observed in positive control (A) and tubes containing TPS/PCL/ATB systems with lower concentrations of ATB (C–E) indicated the growth of bacteria, while the clear solution observed in negative control (B) and tubes containing TPS/PCL/ATB systems releasing higher concentrations of ATB (F–L) proved the bacteriostatic effect, i.e., inhibition of the bacterial growth.
Figure A1. The results of standard microbiological tube dilution tests, in which a defined piece of TPS/PCL/ATB sample (ATB dispersed in TPS phase) was put into a tube containing 5 mL of Mueller-Hinton broth (Oxoid, Brno, Czech Republic); each tube is then inoculated with a standardized bacterial suspension of the CCM 4223 Staphylococcus aureus and observed after 24 h. The turbid solution observed in positive control (A) and tubes containing TPS/PCL/ATB systems with lower concentrations of ATB (C–E) indicated the growth of bacteria, while the clear solution observed in negative control (B) and tubes containing TPS/PCL/ATB systems releasing higher concentrations of ATB (F–L) proved the bacteriostatic effect, i.e., inhibition of the bacterial growth.
Materials 15 01101 g0a1
In the case of above-discussed bone infections, the commercial carrier systems for the local release of antibiotics comprise: (i) bone cement (basically poly(methyl methacrylate)), (ii) capsules of highly porous plaster (CaSO4 2H2O), or (iii) fibrin-based foams [36]. All three systems can contain and release ATB locally, but their mechanical properties are not optimal: bone cement is stiff polymer deep below its glass transition temperature, highly porous plaster is hard and brittle inorganic material, and fibrin foams are too soft. Moreover, the ATB release rate for each of the above-listed materials is constant. In contrast, our TPS/PCL system is a ductile plastic material with tunable mechanical properties, cuttable with scissors at room temperature, shapeable upon heating at 80 °C, and ATB release can be controlled by its composition and morphology. Consequently, TPS/PLC blends are suitable not only as the ATB releasing spacers in bone infections and defects (which was our originally intended application), but also in other biomedical fields, such as sticking plasters, adhesive bandages, and patches for wound healing.

Appendix B. Additional Morphology and Rheology Results

Additional morphology and rheology results supplemented, documented, or verified the conclusions in the main text.
  • Figure A1: The in-situ measurement during the melt-mixing of the TPS/PCL blends confirmed that the addition of PCL to TPS decreased torque moments and processing temperatures even at low PCL concentrations;
  • Figure A2: The comparison of storage (G′) and loss moduli (G″) in oscillatory shear rheometry at 120 °C illustrated that the increasing concentration of TPS gradually increased both stiffness of the system (higher values of G′ and G″) and solid-like nature of the system (G′/G″ ratio). We note that systems with G′ > G″ are regarded as the materials with the predominating solid-like character, while systems with G′ < G″ are regarded as materials with the predominating liquid-like character [48];
  • Figure A3: SEM micrographs of fracture surfaces (prepared in liquid nitrogen) documented a very good compatibility and high interfacial adhesion between TPS and PCL components in the whole concentration range, as the fracture propagated through the particles and not along the interface (see ref. [38] for detailed discussion and examples of fracture surfaces for good and bad interface adhesion).
Materials 15 01101 g0a2 550
Figure A2. SEM micrographs showing smoothed and etched surfaces of selected TPS/PCL blends prepared by two-step preparation procedure (upper row) and three-step preparation procedure (lower row).
Figure A2. SEM micrographs showing smoothed and etched surfaces of selected TPS/PCL blends prepared by two-step preparation procedure (upper row) and three-step preparation procedure (lower row).
Materials 15 01101 g0a2
Materials 15 01101 g0a3 550
Figure A3. Selected examples of the frequency-dependence of storage shear modulus G′ and of the loss shear modulus G″, as observed in oscillatory rheological investigations carried out at 120 °C with TPS/PCL blends; the examples demonstrate the different degree of liquid-like and solid-like character for the blends: (A) TPS/PCL (20/80); (B) TPS/PCL (30/70); (C) TPS/PCL (40/60); and (D) TPS/PCL (80/20).
Figure A3. Selected examples of the frequency-dependence of storage shear modulus G′ and of the loss shear modulus G″, as observed in oscillatory rheological investigations carried out at 120 °C with TPS/PCL blends; the examples demonstrate the different degree of liquid-like and solid-like character for the blends: (A) TPS/PCL (20/80); (B) TPS/PCL (30/70); (C) TPS/PCL (40/60); and (D) TPS/PCL (80/20).
Materials 15 01101 g0a3

Appendix C. Additional Results of Micro- and Macromechanical Measurements

This appendix contains additional plots that further illustrate the results of micromechanical and macromechanical measurements in the main text.
  • Figure A4: Bar plots of micromechanical properties as a function of composition showed that the standard deviations of the measured micromechanical properties were quite low (with the exception of CIT of very soft samples with TPS matrix). Furthermore, they confirmed the strong correlations between all stiffness-related properties (HIT, HM, and EIT);
  • Figure A5: Complete results from DMTA analysis, i.e., the temperature dependence of storage modulus (G′), loss modulus (G″), absolute value of complex modulus (|G*|), and damping factor (tan(δ)) supplemented the data from micromechanical and rheological experiments;
  • Figure A6: Correlations between all macro- and micromechanical properties summarized in the form of heatmap. The heatmap shows Pearson’s correlation coefficients r for all pairs of stiffness-related properties (G′, EIT, HIT and HM) and viscosity-related properties (G″, tan(δ), CIT and ηIT). The strongest linear positive correlations (r close to +1) are marked with a black color, the strongest linear negative correlations (r close to −1) are marked with a red color. The correlation matrix is symmetric with respect to the main diagonal, which contains values equal to 1 (autocorrelations).
Materials 15 01101 g0a4 550
Figure A4. SEM micrographs of fracture surfaces of TPS/PCL blends. The materials were fractured while submerged in liquid nitrogen. The micrographs demonstrate that the fracture propagated mostly through both phases (and not along the interface), which indicates good interfacial adhesion between TPS and PCL components.
Figure A4. SEM micrographs of fracture surfaces of TPS/PCL blends. The materials were fractured while submerged in liquid nitrogen. The micrographs demonstrate that the fracture propagated mostly through both phases (and not along the interface), which indicates good interfacial adhesion between TPS and PCL components.
Materials 15 01101 g0a4
Materials 15 01101 g0a5 550
Figure A5. Selected micromechanical properties of the TPS and PCL and TPS/PCL blends in the form of bar charts with standard deviations: (A) indentation hardness, (B) Martens hardness, (C) indentation modulus and (D) indentation creep.
Figure A5. Selected micromechanical properties of the TPS and PCL and TPS/PCL blends in the form of bar charts with standard deviations: (A) indentation hardness, (B) Martens hardness, (C) indentation modulus and (D) indentation creep.
Materials 15 01101 g0a5
Materials 15 01101 g0a6 550
Figure A6. Complete results of DMTA measurements for all TPS/PCL blends in temperature range −80–160 °C: (A) storage modulus, (B) loss modulus, (C) absolute value of complex modulus, and (D) damping factor.
Figure A6. Complete results of DMTA measurements for all TPS/PCL blends in temperature range −80–160 °C: (A) storage modulus, (B) loss modulus, (C) absolute value of complex modulus, and (D) damping factor.
Materials 15 01101 g0a6
Materials 15 01101 g0a7 550
Figure A7. Correlation matrix table showing Pearson’s coefficients r for all pairs of experimentally determined properties in macro- and microscale. The table is presented as a heatmap (darker color means stronger correlation). The properties in the table are: storage modulus, loss modulus and damping factor from dynamic mechanical thermal analysis (DMTA/G′, DMTA/G″ and DMA/tan(δ)) and elastic modulus, indentation hardness, Martens hardness, indentation creep, and elastic part of indentation work from MHI measurements (MHI/EIT, MHI/HIT, MHI/HM, MHI/CIT and MHI/ηIT). We note that the correlation matrix is symmetric with respect to the main diagonal, which contains values equal to 1 (autocorrelations).
Figure A7. Correlation matrix table showing Pearson’s coefficients r for all pairs of experimentally determined properties in macro- and microscale. The table is presented as a heatmap (darker color means stronger correlation). The properties in the table are: storage modulus, loss modulus and damping factor from dynamic mechanical thermal analysis (DMTA/G′, DMTA/G″ and DMA/tan(δ)) and elastic modulus, indentation hardness, Martens hardness, indentation creep, and elastic part of indentation work from MHI measurements (MHI/EIT, MHI/HIT, MHI/HM, MHI/CIT and MHI/ηIT). We note that the correlation matrix is symmetric with respect to the main diagonal, which contains values equal to 1 (autocorrelations).
Materials 15 01101 g0a7

References

  1. Luckachan, G.E.; Pillai, C.K.S. Biodegradable Polymers—A Review on Recent Trends and Emerging Perspectives. J. Polym. Environ. 2011, 19, 637–676. [Google Scholar] [CrossRef]
  2. Colnik, M.; Knez-Hrncic, M.; Skerget, M.; Knez, Z. Biodegradable Polymers, Current Trends of Research and Their Applications, a Review. Chem. Ind. Chem. Eng. Q. 2020, 26, 401–418. [Google Scholar] [CrossRef]
  3. European Bioplastics. Available online: https://www.european-bioplastics.org/bioplastics/materials/ (accessed on 20 December 2021).
  4. Mohammadi Nafchi, A.; Moradpour, M.; Saeidi, M.; Alias, A.K. Thermoplastic Starches: Properties, Challenges, and Prospects. Starch/Stärke 2013, 65, 61–72. [Google Scholar] [CrossRef]
  5. Liu, H.; Xie, F.; Yu, L.; Chen, L.; Li, L. Thermal Processing of Starch-Based Polymers. Prog. Polym. Sci. 2009, 34, 1348–1368. [Google Scholar] [CrossRef]
  6. Huneault, M.A.; Li, H. Preparation and Properties of Extruded Thermoplastic Starch/Polymer Blends. J. Appl. Polym. Sci. 2012, 126, E96–E108. [Google Scholar] [CrossRef]
  7. Ren, J.; Fu, H.; Ren, T.; Yuan, W. Preparation, Characterization and Properties of Binary and Ternary Blends with Thermoplastic Starch, Poly(Lactic Acid) and Poly(Butylene Adipate-Co-Terephthalate). Carbohydr. Polym. 2009, 77, 576–582. [Google Scholar] [CrossRef]
  8. Rodriguez-Gonzalez, F.J.; Ramsay, B.A.; Favis, B.D. Rheological and Thermal Properties of Thermoplastic Starch with High Glycerol Content. Carbohydr. Polym. 2004, 58, 139–147. [Google Scholar] [CrossRef]
  9. Bootklad, M.; Kaewtatip, K. Biodegradation of Thermoplastic Starch/Eggshell Powder Composites. Carbohydr. Polym. 2013, 97, 315–320. [Google Scholar] [CrossRef]
  10. Matzinos, P.; Tserki, V.; Kontoyiannis, A.; Panayiotou, C. Processing and Characterization of Starch/Polycaprolactone Products. Polym. Degrad. Stab. 2002, 77, 17–24. [Google Scholar] [CrossRef]
  11. Van Der Burgt, M.C.; Van Der Woude, M.E.; Janssen, L.P.B.M. The Influence of Plasticizer on Extruded Thermoplastic Starch. J. Vinyl Addit. Technol. 1996, 2, 170–174. [Google Scholar] [CrossRef] [Green Version]
  12. Ivanič, F.; Jochec-Mošková, D.; Janigová, I.; Chodák, I. Physical Properties of Starch Plasticized by a Mixture of Plasticizers. Eur. Polym. J. 2017, 93, 843–849. [Google Scholar] [CrossRef]
  13. Babaee, M.; Jonoobi, M.; Hamzeh, Y.; Ashori, A. Biodegradability and Mechanical Properties of Reinforced Starch Nanocomposites Using Cellulose Nanofibers. Carbohydr. Polym. 2015, 132, 1–8. [Google Scholar] [CrossRef]
  14. Wang, J.; Cheng, F.; Zhu, P. Structure and Properties of Urea-Plasticized Starch Films with Different Urea Contents. Carbohydr. Polym. 2014, 101, 1109–1115. [Google Scholar] [CrossRef]
  15. Shin, B.-Y.; Lee, S.-I.; Shin, Y.-S.; Balakrishnan, S.; Narayan, R. Rheological, Mechanical and Biodegradation Studies on Blends of Thermoplastic Starch and Polycaprolactone. Polym. Eng. Sci. 2004, 44, 1429–1438. [Google Scholar] [CrossRef]
  16. Ostafińska, A.; Mikešová, J.; Krejčíková, S.; Nevoralová, M.; Šturcová, A.; Zhigunov, A.; Michálková, D.; Šlouf, M. Thermoplastic Starch Composites with TiO2 Particles: Preparation, Morphology, Rheology and Mechanical Properties. Int. J. Biol. Macromol. 2017, 101, 273–282. [Google Scholar] [CrossRef]
  17. Ujcic, A.; Nevoralova, M.; Dybal, J.; Zhigunov, A.; Kredatusova, J.; Krejcikova, S.; Fortelny, I.; Slouf, M. Thermoplastic Starch Composites Filled With Isometric and Elongated TiO2-Based Nanoparticles. Front. Mater. 2019, 6, 284. [Google Scholar] [CrossRef]
  18. Ujcic, A.; Krejcikova, S.; Nevoralova, M.; Zhigunov, A.; Dybal, J.; Krulis, Z.; Fulin, P.; Nyc, O.; Slouf, M. Thermoplastic Starch Composites With Titanium Dioxide and Vancomycin Antibiotic: Preparation, Morphology, Thermomechanical Properties, and Antimicrobial Susceptibility Testing. Front. Mater. 2020, 7, 9. [Google Scholar] [CrossRef] [Green Version]
  19. Kaseem, M.; Hamad, K.; Deri, F. Thermoplastic Starch Blends: A Review of Recent Works. Polym. Sci. Ser. A 2012, 54, 165–176. [Google Scholar] [CrossRef]
  20. Mina Hernandez, J.H. Effect of the Incorporation of Polycaprolactone (PCL) on the Retrogradation of Binary Blends with Cassava Thermoplastic Starch (TPS). Polymers 2020, 13, 38. [Google Scholar] [CrossRef]
  21. Labus, K.; Trusek-Holownia, A.; Semba, D.; Ostrowska, J.; Tynski, P.; Bogusz, J. Biodegradable Polylactide and Thermoplastic Starch Blends as Drug Release Device—Mass Transfer Study. Pol. J. Chem. Technol. 2018, 20, 75–80. [Google Scholar] [CrossRef] [Green Version]
  22. Martin, O.; Avérous, L. Poly(Lactic Acid): Plasticization and Properties of Biodegradable Multiphase Systems. Polymer 2001, 42, 6209–6219. [Google Scholar] [CrossRef]
  23. Sarazin, P.; Li, G.; Orts, W.J.; Favis, B.D. Binary and Ternary Blends of Polylactide, Polycaprolactone and Thermoplastic Starch. Polymer 2008, 49, 599–609. [Google Scholar] [CrossRef]
  24. Bou-Francis, A.; Piercey, M.; Al-Qatami, O.; Mazzanti, G.; Khattab, R.; Ghanem, A. Polycaprolactone Blends for Fracture Fixation in Low Load-bearing Applications. J. Appl. Polym. Sci. 2020, 137, 48940. [Google Scholar] [CrossRef]
  25. Mano, J.F.; Niarova, D.K. Thermal Properties of Thermoplastic Starch/Synthetic Polymer Blends with Potential Biomedical Applicability. J. Mater. Sci. Mater. Med. 2003, 14, 127–135. [Google Scholar] [CrossRef]
  26. Russo, M.A.L.; O’Sullivan, C.; Rounsefell, B.; Halley, P.J.; Truss, R.; Clarke, W.P. The Anaerobic Degradability of Thermoplastic Starch: Polyvinyl Alcohol Blends: Potential Biodegradable Food Packaging Materials. Bioresour. Technol. 2009, 100, 1705–1710. [Google Scholar] [CrossRef]
  27. Bastioli, C.; Bellotti, V.; Giudice, L.; Gilli, G. Mater-Bi: Properties and Biodegradability. J. Environ. Polym. Degrad. 1993, 1, 181–191. [Google Scholar] [CrossRef]
  28. Bastioli, C. Properties and Applications of Mater-Bi Starch-Based Materials. Polym. Degrad. Stab. 1998, 59, 263–272. [Google Scholar] [CrossRef]
  29. Lörcks, J. Properties and Applications of Compostable Starch-Based Plastic Material. Polym. Degrad. Stab. 1998, 59, 245–249. [Google Scholar] [CrossRef]
  30. Quiles-Carrillo, L.; Montanes, N.; Pineiro, F.; Jorda-Vilaplana, A.; Torres-Giner, S. Ductility and Toughness Improvement of Injection-Molded Compostable Pieces of Polylactide by Melt Blending with Poly(ε-Caprolactone) and Thermoplastic Starch. Materials 2018, 11, 2138. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  31. Guarás, M.P.; Alvarez, V.A.; Ludueña, L.N. Processing and Characterization of Thermoplastic Starch/Polycaprolactone/Compatibilizer Ternary Blends for Packaging Applications. J. Polym. Res. 2015, 22, 165. [Google Scholar] [CrossRef]
  32. Diaz, C.A.; Shah, R.K.; Evans, T.; Trabold, T.A.; Draper, K. Thermoformed Containers Based on Starch and Starch/Coffee Waste Biochar Composites. Energies 2020, 13, 6034. [Google Scholar] [CrossRef]
  33. Gheorghita, R.; Anchidin-Norocel, L.; Filip, R.; Dimian, M.; Covasa, M. Applications of Biopolymers for Drugs and Probiotics Delivery. Polymers 2021, 13, 2729. [Google Scholar] [CrossRef] [PubMed]
  34. Balmayor, E.R.; Tuzlakoglu, K.; Azevedo, H.S.; Reis, R.L. Preparation and Characterization of Starch-Poly-ε-Caprolactone Microparticles Incorporating Bioactive Agents for Drug Delivery and Tissue Engineering Applications. Acta Biomater. 2009, 5, 1035–1045. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Masters, E.A.; Trombetta, R.P.; de Mesy Bentley, K.L.; Boyce, B.F.; Gill, A.L.; Gill, S.R.; Nishitani, K.; Ishikawa, M.; Morita, Y.; Ito, H.; et al. Evolving Concepts in Bone Infection: Redefining “Biofilm”, “Acute vs. Chronic Osteomyelitis”, “the Immune Proteome” and “Local Antibiotic Therapy”. Bone Res. 2019, 7, 20. [Google Scholar] [CrossRef] [Green Version]
  36. Slouf, M.; Krulis, Z.; Ostafinska, A.; Nevoralova, M.; Krejcikova, S. Polymerní Termoplastická Biodegradovatelná Kompozice Pro Výrobu Vložek k Léčení a Prevenci Lokálních Infektů a Způsob Její Přípravy. Czech Patent CZ 307056, 8 November 2017. [Google Scholar]
  37. Fortelný, I.; Šlouf, M.; Sikora, A.; Hlavatá, D.; Hašová, V.; Mikešová, J.; Jacob, C. The Effect of the Architecture and Concentration of Styrene–Butadiene Compatibilizers on the Morphology of Polystyrene/Low-Density Polyethylene Blends. J. Appl. Polym. Sci. 2006, 100, 2803–2816. [Google Scholar] [CrossRef]
  38. Kalasova, D.; Zikmund, T.; Pina, L.; Takeda, Y.; Horvath, M.; Omote, K.; Kaiser, J. Characterization of a Laboratory-Based X-Ray Computed Nanotomography System for Propagation-Based Method of Phase Contrast Imaging. IEEE Trans. Instrum. Meas. 2020, 69, 1170–1178. [Google Scholar] [CrossRef]
  39. Lifton, J.J.; Liu, T. Evaluation of the Standard Measurement Uncertainty Due to the ISO50 Surface Determination Method for Dimensional Computed Tomography. Precis. Eng. 2020, 61, 82–92. [Google Scholar] [CrossRef]
  40. Slouf, M.; Strachota, B.; Strachota, A.; Gajdosova, V.; Bertschova, V.; Nohava, J. Macro-, Micro- and Nanomechanical Characterization of Crosslinked Polymers with Very Broad Range of Mechanical Properties. Polymers 2020, 12, 2951. [Google Scholar] [CrossRef]
  41. Slouf, M.; Krajenta, J.; Gajdosova, V.; Pawlak, A. Macromechanical and Micromechanical Properties of Polymers with Reduced Density of Entanglements. Polym. Eng. Sci. 2021, 61, 1773–1790. [Google Scholar] [CrossRef]
  42. Samara, E.; Moriarty, T.F.; Decosterd, L.A.; Richards, R.G.; Gautier, E.; Wahl, P. Antibiotic Stability over Six Weeks in Aqueous Solution at Body Temperature with and without Heat Treatment That Mimics the Curing of Bone Cement. Bone Jt. Res. 2017, 6, 296–306. [Google Scholar] [CrossRef]
  43. Carli, A.V.; Sethuraman, A.S.; Bhimani, S.J.; Ross, F.P.; Bostrom, M.P.G. Selected Heat-Sensitive Antibiotics Are Not Inactivated During Polymethylmethacrylate Curing and Can Be Used in Cement Spacers for Periprosthetic Joint Infection. J. Arthroplasty 2018, 33, 1930–1935. [Google Scholar] [CrossRef]
  44. Traub, W.H.; Leonhard, B. Heat Stability of the Antimicrobial Activity of Sixty-Two Antibacterial Agents. J. Antimicrob. Chemother. 1995, 35, 149–154. [Google Scholar] [CrossRef]
  45. Dwivedi, C.; Pandey, H.; Pandey, A.; Ramteke, P. Fabrication and Assessment of Gentamicin Loaded Electrospun Nanofibrous Scaffolds as a Quick Wound Healing Dressing Material. Curr. Nanosci. 2015, 11, 222–228. [Google Scholar] [CrossRef]
  46. Isaev, A.I. Encyclopedia of Polymer Blends; Wiley-VCH Verlag GmbH & Co. KGaA: Weinheim, Germany, 2011; ISBN 978-3-527-31930-5. [Google Scholar]
  47. Mezger, T.G. The Rheology Handbook, 4th ed.; Vincentz Network: Hanover, Germany, 2014; ISBN 978-3-7486-0036-7. [Google Scholar]
  48. Šlouf, M.; Kolařík, J.; Kotek, J. Rubber-Toughened Polypropylene/Acrylonitrile-Co-Butadiene-Co-Styrene Blends: Morphology and Mechanical Properties. Polym. Eng. Sci. 2007, 47, 582–592. [Google Scholar] [CrossRef]
  49. Vacková, T.; Slouf, M.; Nevoralová, M.; Kaprálková, L. HDPE/COC Blends with Fibrous Morphology and Their Properties. Eur. Polym. J. 2012, 48, 2031–2039. [Google Scholar] [CrossRef]
  50. Ostafinska, A.; Vackova, T.; Slouf, M. Strong Synergistic Improvement of Mechanical Properties in HDPE/COC Blends with Fibrillar Morphology. Polym. Eng. Sci. 2018, 58, 1955–1964. [Google Scholar] [CrossRef]
  51. Paul, D.R.; Barlow, J.W. Polymer Blends. J. Macromol. Sci. Part C 1980, 18, 109–168. [Google Scholar] [CrossRef]
  52. Oliver, W.C.; Pharr, G.M. Nanoindentation in Materials Research: Past, Present, and Future. MRS Bull. 2010, 35, 897–907. [Google Scholar] [CrossRef]
  53. Ward, I.M.; Sweeney, J. An Introduction to the Mechanical Properties of Solid Polymers, 2nd ed.; Wiley: Chichester, UK, 2004; ISBN 978-0-471-49625-0. [Google Scholar]
  54. Baltá Calleja, F.J.; Fakirov, S. Microhardness of Polymers, 1st ed.; Cambridge University Press: Cambridge, UK, 2000; ISBN 978-0-521-64218-7. [Google Scholar]
  55. Slouf, M.; Pavlova, E.; Krejcikova, S.; Ostafinska, A.; Zhigunov, A.; Krzyzanek, V.; Sowinski, P.; Piorkowska, E. Relations between Morphology and Micromechanical Properties of Alpha, Beta and Gamma Phases of IPP. Polym. Test. 2018, 67, 522–532. [Google Scholar] [CrossRef]
  56. Nielsen, L.E.; Landel, R.F. Mechanical Properties of Polymers and Composites, 2nd ed.; M. Dekker: New York, NY, USA, 1994; ISBN 978-0-8247-8964-0. [Google Scholar]
  57. Kolarik, J. Simultaneous Prediction of the Modulus and Yield Strength of Binary Polymer Blends. Polym. Eng. Sci. 1996, 36, 2518–2524. [Google Scholar] [CrossRef]
  58. Slouf, M.; Ujcic, A.; Nevoralova, M.; Vackova, T.; Fambri, L.; Kelnar, I. Monitoring of Morphology and Properties During Preparation of PCL/PLA Microfibrillar Composites With Organophilic Montmorillonite. Front. Mater. 2020, 7, 188. [Google Scholar] [CrossRef]
  59. Tabor, D. The Hardness of Metals; Oxford Classic Texts in the Physical Sciences; Oxford University Press: Oxford, UK, 1951; ISBN 978-0-598-84515-3. [Google Scholar]
  60. Pegoretti, A.; Kolarík, J.; Fambri, L.; Penati, A. Polypropylene/Cycloolefin Copolymer Blends: Effects of Fibrous Phase Structure on Tensile Mechanical Properties. Polymer 2003, 44, 3381–3387. [Google Scholar] [CrossRef]
  61. Averous, L. Properties of Thermoplastic Blends: Starch–Polycaprolactone. Polymer 2000, 41, 4157–4167. [Google Scholar] [CrossRef]
  62. Li, G.; Favis, B.D. Morphology Development and Interfacial Interactions in Polycaprolactone/Thermoplastic-Starch Blends: Morphology Development and Interfacial Interactions. Macromol. Chem. Phys. 2010, 211, 321–333. [Google Scholar] [CrossRef]
  63. Herrmann, K. (Ed.) Hardness Testing: Principles and Applications; ASM International: Russell, OH, USA, 2011; ISBN 978-1-61503-832-9. [Google Scholar]
  64. Mahieu, A.; Terrié, C.; Agoulon, A.; Leblanc, N.; Youssef, B. Thermoplastic Starch and Poly(ε-Caprolactone) Blends: Morphology and Mechanical Properties as a Function of Relative Humidity. J. Polym. Res. 2013, 20, 229. [Google Scholar] [CrossRef]
  65. Ali Akbari Ghavimi, S.; Ebrahimzadeh, M.H.; Solati-Hashjin, M.; Abu Osman, N.A. Polycaprolactone/Starch Composite: Fabrication, Structure, Properties, and Applications. J. Biomed. Mater. Res. 2015, 103, 2482–2498. [Google Scholar] [CrossRef]
  66. Labet, M.; Thielemans, W. Synthesis of Polycaprolactone: A Review. Chem. Soc. Rev. 2009, 38, 3484. [Google Scholar] [CrossRef]
  67. Leja, K.; Lewandowicz, G. Polymer Biodegradation and Biodegradable Polymers—A Review. Pol. J. Environ. Stud. 2010, 19, 255–266. [Google Scholar]
Materials 15 01101 g001 550
Figure 1. Principle of MHI measurements, which were employed in this work: (a) Micromechanical properties were deduced from the experimental F–h curves (where F is the indenter loading force and h is the indenter penetration depth) by means of (b) formulas and (c) relations containing experimental parameters, such as maximum loading force (Fmax), slope at the beginning of the unloading curve (S), penetration depths at the beginning and end of the maximal load (h1 and h2), and areas under loading and unloading curve (Welast and Wtotal). Additional parameter, contact depth (hc), was calculated in terms of the Oliver and Pharr theory and employed in the calculation of EIT and HIT, as described in our previous work [41,42]. The figure shows real, representative F–h curves of TPS/PCL systems, which the illustrate substantial changes of all studied properties as a function of composition.
Figure 1. Principle of MHI measurements, which were employed in this work: (a) Micromechanical properties were deduced from the experimental F–h curves (where F is the indenter loading force and h is the indenter penetration depth) by means of (b) formulas and (c) relations containing experimental parameters, such as maximum loading force (Fmax), slope at the beginning of the unloading curve (S), penetration depths at the beginning and end of the maximal load (h1 and h2), and areas under loading and unloading curve (Welast and Wtotal). Additional parameter, contact depth (hc), was calculated in terms of the Oliver and Pharr theory and employed in the calculation of EIT and HIT, as described in our previous work [41,42]. The figure shows real, representative F–h curves of TPS/PCL systems, which the illustrate substantial changes of all studied properties as a function of composition.
Materials 15 01101 g001
Materials 15 01101 g002 550
Figure 2. Torque moments as a function of melt-mixing time and maximum temperatures during melt-mixing of neat TPS (black line) and TPS/PCL (70/30) blend (blue line). The initial increase of the torque moments corresponds to the filling of the mixing chamber, which was heated to 120 °C.
Figure 2. Torque moments as a function of melt-mixing time and maximum temperatures during melt-mixing of neat TPS (black line) and TPS/PCL (70/30) blend (blue line). The initial increase of the torque moments corresponds to the filling of the mixing chamber, which was heated to 120 °C.
Materials 15 01101 g002
Materials 15 01101 g003 550
Figure 3. Absolute value of the complex viscosity (|η*|) as a function of oscillatory shear angular frequency (ω) during the oscillatory shear measurements for all TPS/PCL blends.
Figure 3. Absolute value of the complex viscosity (|η*|) as a function of oscillatory shear angular frequency (ω) during the oscillatory shear measurements for all TPS/PCL blends.
Materials 15 01101 g003
Materials 15 01101 g004 550
Figure 4. SEM micrographs showing smoothed surfaces of the TPS/PCL blends; the TPS phase was etched off by hydrochloric acid.
Figure 4. SEM micrographs showing smoothed surfaces of the TPS/PCL blends; the TPS phase was etched off by hydrochloric acid.
Materials 15 01101 g004
Materials 15 01101 g005 550
Figure 5. Phase co-continuity in TPS/PCL blends: (a) a theoretical prediction of the TPS/PCL composition exhibiting co-continuous morphology and (b) an experimental proof of co-continuity in the TPS/PCL (70/30) blend by means of subμ-CT measurement. The theoretical prediction (a) suggested that co-continuous morphology should appear at higher concentrations of TPS (as explained in Section 3.1.4). The experimental verification of the co-continuity of the TPS/PCL (70/30) blend (b) is displayed in the form of a 3D reconstruction of 100 × 100 × 100 μm3 volume of the sample measured by subμ-CT. The model illustrates co-continuity in both phases. The etched-off phase (TPS; shown in blue) forms interconnected pores. The connections among the pores can be observed in the thin slices on the right.
Figure 5. Phase co-continuity in TPS/PCL blends: (a) a theoretical prediction of the TPS/PCL composition exhibiting co-continuous morphology and (b) an experimental proof of co-continuity in the TPS/PCL (70/30) blend by means of subμ-CT measurement. The theoretical prediction (a) suggested that co-continuous morphology should appear at higher concentrations of TPS (as explained in Section 3.1.4). The experimental verification of the co-continuity of the TPS/PCL (70/30) blend (b) is displayed in the form of a 3D reconstruction of 100 × 100 × 100 μm3 volume of the sample measured by subμ-CT. The model illustrates co-continuity in both phases. The etched-off phase (TPS; shown in blue) forms interconnected pores. The connections among the pores can be observed in the thin slices on the right.
Materials 15 01101 g005
Materials 15 01101 g006 550
Figure 6. Micromechanical properties of TPS, PCL, and of the TPS/PCL blends (red points) vs. theoretical predictions (black lines). The plots show (A) indentation hardness, (B) indentation modulus, (C) indentation creep and (D) elastic work of indentation. The theoretical predictions comprise linear model (LIN; the final blend property is a linear combination of the blend component properties) and equivalent box model (EBM; a simple predictive scheme for isotropic polymer blends, which considers phase continuity and interfacial adhesion). The models are described in Section 3.2.1.
Figure 6. Micromechanical properties of TPS, PCL, and of the TPS/PCL blends (red points) vs. theoretical predictions (black lines). The plots show (A) indentation hardness, (B) indentation modulus, (C) indentation creep and (D) elastic work of indentation. The theoretical predictions comprise linear model (LIN; the final blend property is a linear combination of the blend component properties) and equivalent box model (EBM; a simple predictive scheme for isotropic polymer blends, which considers phase continuity and interfacial adhesion). The models are described in Section 3.2.1.
Materials 15 01101 g006
Materials 15 01101 g007 550
Figure 7. Results of DMTA: storage modulus (G′) is a function of temperature (T) for all TPS/PCL blends. The inset shows detail of the region around room temperature.
Figure 7. Results of DMTA: storage modulus (G′) is a function of temperature (T) for all TPS/PCL blends. The inset shows detail of the region around room temperature.
Materials 15 01101 g007
Materials 15 01101 g008 550
Figure 8. Scatterplot matrix graph showing correlation between selected micromechanical (G′) and micromechanical properties (EIT, HIT and HM). Diagonal elements of the scatterplot matrix graph show distribution of the measured quantities, whereas off-diagonal elements show correlations between each pair of quantities. The translucent bands around the regression lines represent 95% confidence interval of the regression estimate. Moreover, all off-diagonal plots show the values of Pearson’s correlation coefficient r in the upper right corner.
Figure 8. Scatterplot matrix graph showing correlation between selected micromechanical (G′) and micromechanical properties (EIT, HIT and HM). Diagonal elements of the scatterplot matrix graph show distribution of the measured quantities, whereas off-diagonal elements show correlations between each pair of quantities. The translucent bands around the regression lines represent 95% confidence interval of the regression estimate. Moreover, all off-diagonal plots show the values of Pearson’s correlation coefficient r in the upper right corner.
Materials 15 01101 g008
Table
Table 1. List of the prepared TPS/PCL blends; each blend was prepared by both two-step procedure (Section 2.2.2) and three-step procedure (Section 2.2.3).
Table 1. List of the prepared TPS/PCL blends; each blend was prepared by both two-step procedure (Section 2.2.2) and three-step procedure (Section 2.2.3).
Sample IDWt.% (TPS)Wt.% (PCL)
TPS/PCL (0/100)0100
TPS/PCL (10/90)1090
TPS/PCL (20/80)2080
TPS/PCL (30/70)3070
TPS/PCL (40/60)4060
TPS/PCL (50/50)5050
TPS/PCL (60/40)6040
TPS/PCL (70/30)7030
TPS/PCL (80/20)8020
TPS/PCL (90/10)9010
TPS/PCL (100/0)1000
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Gajdosova, V.; Strachota, B.; Strachota, A.; Michalkova, D.; Krejcikova, S.; Fulin, P.; Nyc, O.; Brinek, A.; Zemek, M.; Slouf, M. Biodegradable Thermoplastic Starch/Polycaprolactone Blends with Co-Continuous Morphology Suitable for Local Release of Antibiotics. Materials 2022, 15, 1101. https://doi.org/10.3390/ma15031101

AMA Style

Gajdosova V, Strachota B, Strachota A, Michalkova D, Krejcikova S, Fulin P, Nyc O, Brinek A, Zemek M, Slouf M. Biodegradable Thermoplastic Starch/Polycaprolactone Blends with Co-Continuous Morphology Suitable for Local Release of Antibiotics. Materials. 2022; 15(3):1101. https://doi.org/10.3390/ma15031101

Chicago/Turabian Style

Gajdosova, Veronika, Beata Strachota, Adam Strachota, Danuse Michalkova, Sabina Krejcikova, Petr Fulin, Otakar Nyc, Adam Brinek, Marek Zemek, and Miroslav Slouf. 2022. "Biodegradable Thermoplastic Starch/Polycaprolactone Blends with Co-Continuous Morphology Suitable for Local Release of Antibiotics" Materials 15, no. 3: 1101. https://doi.org/10.3390/ma15031101

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop