How Surface Properties of Silica Nanoparticles Influence Structural, Microstructural and Biological Properties of Polymer Nanocomposites
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions
- the particles of the high specific surface silica (hss-SiO2) in polymer matrix (PLDLA) significantly enhanced the formation of apatite-like structures at the surface during a bioactivity test (in vitro conditions); this feature can be monitored by zeta potential in SBF;
- the nanofillers such as hss-SiO2 significantly influence physicochemical properties such as wetting contact angle and surface free energy of polymer matrix, and slightly influence the crystallinity of nanocomposite materials (PLDLA/hss-SiO2). These parameters may be relevant to the biological response from both the mesenchymal and somatic cells;
- the results of biological investigations, realised with the use of mesenchymal stem cells, show that the bioactive nanocomposite where hss-SiO2 was used as a filler may stimulate differentiation of mesenchymal cells into osteoblasts—resulting in higher proliferation stage of cells in in vitro conditions and higher alkaline phosphatase activity. Somatic cells: fibroblast and osteoblast contacted with nanocomposite with hss-SiO2 confirm that this material is much more suitable for promotion of cells’ proliferation than PLDLA/lss-SiO2.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fluid | Concentration of Ion (mM) | |||||||
---|---|---|---|---|---|---|---|---|
Cl− | HCO3− | HPO42− | SO42− | Na+ | K+ | Mg2+ | Ca2+ | |
Body Plasma | 103.0 | 27.0 | 1.0 | 0.5 | 142.0 | 5.0 | 1.5 | 2.5 |
Simulated Body Fluid, SBF | 148.8 | 4.2 | 1.0 | 0.5 | 142.0 | 5.0 | 1.5 | 2.5 |
Nanoparticles Characteristics | hss-SiO2 | lss-SiO2 |
---|---|---|
Modal particle diameter, DLS (nm) | 220 | 142 |
Mean particle size, TEM (nm) | 60 | 50 |
Specific surface area, BET (m2/g) | 582.8 | 65.8 |
Material | Tg, °C | Tm, °C | Tdeg, °C | λ, % |
---|---|---|---|---|
PL/DLA | 57.3 | 162.5 | 356.7 | 36.5 |
PL/DLA hss-SiO2 | 59.8 | 168.2 | 350.3 | 40.2 |
PL/DLA lss-SiO2 | 57.2 | 161.8 | 348.2 | 37.6 |
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Zych, Ł.; Osyczka, A.M.; Łacz, A.; Różycka, A.; Niemiec, W.; Rapacz-Kmita, A.; Dzierzkowska, E.; Stodolak-Zych, E. How Surface Properties of Silica Nanoparticles Influence Structural, Microstructural and Biological Properties of Polymer Nanocomposites. Materials 2021, 14, 843. https://doi.org/10.3390/ma14040843
Zych Ł, Osyczka AM, Łacz A, Różycka A, Niemiec W, Rapacz-Kmita A, Dzierzkowska E, Stodolak-Zych E. How Surface Properties of Silica Nanoparticles Influence Structural, Microstructural and Biological Properties of Polymer Nanocomposites. Materials. 2021; 14(4):843. https://doi.org/10.3390/ma14040843
Chicago/Turabian StyleZych, Łukasz, Anna Maria Osyczka, Agnieszka Łacz, Agnieszka Różycka, Wiktor Niemiec, Alicja Rapacz-Kmita, Ewa Dzierzkowska, and Ewa Stodolak-Zych. 2021. "How Surface Properties of Silica Nanoparticles Influence Structural, Microstructural and Biological Properties of Polymer Nanocomposites" Materials 14, no. 4: 843. https://doi.org/10.3390/ma14040843