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Open AccessArticle

Release of the Non-Steroidal Anti-Inflammatory Drug Flufenamic Acid by Multiparticulate Delivery Systems Promotes Adipogenic Differentiation of Adipose-Derived Stem Cells

1
Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, Romania
2
Research Institute of the University of Bucharest, 050663 Bucharest, Romania
3
Department of Collagen Research, Division of Leather and Footwear Research Institute, The National Research & Development Institute for Textiles and Leather, 031215 Bucharest, Romania
4
Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania
*
Author to whom correspondence should be addressed.
Materials 2020, 13(7), 1550; https://doi.org/10.3390/ma13071550
Received: 22 January 2020 / Revised: 21 March 2020 / Accepted: 25 March 2020 / Published: 27 March 2020
(This article belongs to the Special Issue Advanced Nanosciences Applied in Tissue Engineering and Drug Delivery)
Engineered tissue-like structures often instigate an inflammatory response in the host that can inhibit wound healing and ultimately lead to the rejection of the implant. In our previous study, we have characterized the properties and biocompatibility of novel multiparticulate drug delivery systems (MDDS), based on collagen matrix with gradual release of anti-inflammatory drug flufenamic acid, we evaluated their anti-inflammatory potential and demonstrated their efficiency against burns and soft tissue lesions. In addition to these results, FA was previously described as a stimulant for adipogenesis, therefore we hypothesized that MDDS might also be appropriate for adipose tissue engineering. After the cell-scaffold constructs were obtained, cell morphology, adhesion and spreading on the systems were highlighted by scanning electron microscopy, immunostaining and confocal microscopy. The effect of FA-enriched materials on adipogenesis was evaluated at gene and protein level, by RT-qPCR, confocal microscopy and immunohistochemistry. Our current work indicates that flufenamic acid plays a beneficial role in adipocyte differentiation, with a direct effect upon the gene and protein expression of important early and late markers of adipogenesis, such as PPARγ2 and perilipin. View Full-Text
Keywords: adipose tissue engineering; multiparticulate drug delivery systems; flufenamic acid; adipose-derived stem cells; adipogenic differentiation; PPARγ2; perilipin adipose tissue engineering; multiparticulate drug delivery systems; flufenamic acid; adipose-derived stem cells; adipogenic differentiation; PPARγ2; perilipin
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Lazăr, A.D.; Dinescu, S.; Albu-Kaya, M.G.; Gharbia, S.; Hermenean, A.; Costache, M. Release of the Non-Steroidal Anti-Inflammatory Drug Flufenamic Acid by Multiparticulate Delivery Systems Promotes Adipogenic Differentiation of Adipose-Derived Stem Cells. Materials 2020, 13, 1550.

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