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Article

Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles

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Biosystems and Biomaterials Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA
2
Chemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA
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Protein and Cell Analysis, Thermo Fisher Scientific, Eugene, OR 97402, USA
*
Author to whom correspondence should be addressed.
Materials 2020, 13(18), 4111; https://doi.org/10.3390/ma13184111
Received: 4 August 2020 / Revised: 9 September 2020 / Accepted: 10 September 2020 / Published: 16 September 2020
(This article belongs to the Special Issue Fluorescent Probes for Imaging and Detection)
The National Institute of Standards and Technology (NIST), the National Institutes of Health (NIH) and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative equivalent reference fluorophore (ERF) values with high accuracy and precision. The ultimate goal of this effort is to accurately quantify the number of antibodies bound to individual living cells. The expansion of this effort to assign ERF values to more than 50 fluorescence channels and particles with diameters ranging from 10 μm down to 80 nm is reported here. View Full-Text
Keywords: beads; calibration; cytometer; flow cytometry; fluorescence channels; fluorophores; microspheres; nanospheres; NIST; reference materials; standards beads; calibration; cytometer; flow cytometry; fluorescence channels; fluorophores; microspheres; nanospheres; NIST; reference materials; standards
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MDPI and ACS Style

DeRose, P.; Tian, L.; Elsheikh, E.; Urbas, A.; Zhang, Y.-Z.; Wang, L. Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles. Materials 2020, 13, 4111. https://doi.org/10.3390/ma13184111

AMA Style

DeRose P, Tian L, Elsheikh E, Urbas A, Zhang Y-Z, Wang L. Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles. Materials. 2020; 13(18):4111. https://doi.org/10.3390/ma13184111

Chicago/Turabian Style

DeRose, Paul, Linhua Tian, Elzafir Elsheikh, Aaron Urbas, Yu-Zhong Zhang, and Lili Wang. 2020. "Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles" Materials 13, no. 18: 4111. https://doi.org/10.3390/ma13184111

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