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Article

Enzyme-Catalyzed Production of Potato Galactan-Oligosaccharides and Its Optimization by Response Surface Methodology

1
Centro de Investigación en Alimentación y Desarrollo A. C, Culiacán 80129, Sinaloa, Mexico
2
Centro de Graduados e Investigación en Química, Tecnológico Nacional de México/Instituto Tecnológico de Tijuana, Apartado Postal 1166, Tijuana, Baja California 22510, Mexico
3
Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán 80013, Sinaloa, Mexico
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Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo Coahuila 25000, Mexico
5
Plant Sciences Department, University of California, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Materials 2019, 12(9), 1465; https://doi.org/10.3390/ma12091465
Received: 26 March 2019 / Revised: 25 April 2019 / Accepted: 26 April 2019 / Published: 7 May 2019
This work shows an optimized enzymatic hydrolysis of high molecular weight potato galactan yielding pectic galactan-oligosaccharides (PGOs), where endo-β-1,4-galactanase (galactanase) from Cellvibrio japonicus and Clostridium thermocellum was used. For this, response surface methodology (RSM) by central composite design (CCD) was applied. The parameters varied were temperature (°C), pH, incubation time (min), and enzyme/substrate ratio (U/mg). The optimized conditions for the production of low degree of polymerization (DP) PGOs were obtained for each enzyme by spectrophotometric assay and confirmed by chromatography. The optimal conditions predicted for the use of C. japonicus galactanase to obtain PGOs of DP = 2 were T = 51.8 °C, pH 5, E/S = 0.508 U/mg, and t = 77.5 min. For DP = 3, they were T = 21 °C, pH 9, E/S = 0.484 U/mg, and t = 12.5 min; and for DP = 4, they were T = 21 °C, pH 5, E/S = 0.462 U/mg, and t = 12.5 min. The efficiency results were 51.3% for substrate hydrolysis. C. thermocellum galactanase had a lower yield (35.7%) and optimized conditions predicted for PGOs of DP = 2 were T = 60 °C, pH 5, E/S = 0.525 U/mg, and time = 148 min; DP = 3 were T = 59.7 °C, pH 5, E/S = 0.506 U/mg, and time = 12.5 min; and DP = 4, were T = 34.5 °C, pH 11, E/S = 0.525 U/mg, and time = 222.5 min. Fourier transformed infrared (FT-IR) and nuclear magnetic resonance (NMR) characterizations of PGOs are presented. View Full-Text
Keywords: pectic galactan; enzymatic hydrolysis; endo-β-1,4-galactanase pectic galactan; enzymatic hydrolysis; endo-β-1,4-galactanase
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MDPI and ACS Style

González-Ayón, M.A.; Licea-Claveríe, Á.; Valdez-Torres, J.B.; Picos-Corrales, L.A.; Vélez-de la Rocha, R.; Contreras-Esquivel, J.C.; Labavitch, J.M.; Sañudo-Barajas, J.A. Enzyme-Catalyzed Production of Potato Galactan-Oligosaccharides and Its Optimization by Response Surface Methodology. Materials 2019, 12, 1465. https://doi.org/10.3390/ma12091465

AMA Style

González-Ayón MA, Licea-Claveríe Á, Valdez-Torres JB, Picos-Corrales LA, Vélez-de la Rocha R, Contreras-Esquivel JC, Labavitch JM, Sañudo-Barajas JA. Enzyme-Catalyzed Production of Potato Galactan-Oligosaccharides and Its Optimization by Response Surface Methodology. Materials. 2019; 12(9):1465. https://doi.org/10.3390/ma12091465

Chicago/Turabian Style

González-Ayón, Mirian A., Ángel Licea-Claveríe, José B. Valdez-Torres, Lorenzo A. Picos-Corrales, Rosabel Vélez-de la Rocha, Juan C. Contreras-Esquivel, John M. Labavitch, and Josefa A. Sañudo-Barajas. 2019. "Enzyme-Catalyzed Production of Potato Galactan-Oligosaccharides and Its Optimization by Response Surface Methodology" Materials 12, no. 9: 1465. https://doi.org/10.3390/ma12091465

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