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Materials 2019, 12(7), 1180; https://doi.org/10.3390/ma12071180

A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors

1
Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Louis Pasteur St., 400349 Cluj-Napoca, Romania
2
Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne St., 5000 Monastir, Tunisia
3
Department of Oral Rehabilitation, Oral Health and Dental Office Management, Faculty of Dentistry, Iuliu Haţieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Authors with equal contributions.
Received: 24 March 2019 / Revised: 8 April 2019 / Accepted: 9 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Graphene Oxide: Synthesis, Reduction, and Frontier Applications)
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Abstract

Pyoverdine is a fluorescent siderophore produced by Pseudomonas aeruginosa that can be considered as a detectable marker in nosocomial infections. The presence of pyoverdine in water can be directly linked to the presence of the P. aeruginosa, thus being a nontoxic and low-cost marker for the detection of biological contamination. A novel platform was developed and applied for the electrochemical selective and sensitive detection of pyoverdine, based on a graphene/graphite-modified screen-printed electrode (SPE) that was electrochemically reduced and decorated with gold nanoparticles (NPs). The optimized sensor presenting higher sensitivity towards pyoverdine was successfully applied for its detection in real samples (serum, saliva, and tap water), in the presence of various interfering species. The excellent analytical performances underline the premises for an early diagnosis kit of bacterial infections based on electrochemical sensors. View Full-Text
Keywords: Pseudomonas aeruginosa; pyoverdine; graphene; electrochemical sensor; virulence; factors Pseudomonas aeruginosa; pyoverdine; graphene; electrochemical sensor; virulence; factors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Gandouzi, I.; Tertis, M.; Cernat, A.; Saidane-Mosbahi, D.; Ilea, A.; Cristea, C. A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors. Materials 2019, 12, 1180.

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